DOI: 10.1139/w03-078. Putative virulence factors are released in association with membrane vesicles from<i>Burkholderia cepacia</i>

Putative virulence factors are released in association with membrane vesicles fromBurkholderia cepacia

10.1139/w03-078

Crossref journal-article

Canadian Science Publishing

Canadian Journal of Microbiology (155)

Abstract

Like many other Gram-negative bacteria, Burkholderia cepacia naturally releases membrane vesicles (n-MVs) during normal growth. Through filtration and differential centrifugation, n-MVs from clinical isolates of the IIIa and V genomovars were isolated and their characteristics compared. Electron microscopy revealed that they were spherical, 30220 nm in diameter, and bilayered. Virulence factors thought to play a role in pathogenicity (e.g., lipase, phospholipase-N, and protease, including a metalloprotease) were found associated with n-MVs, while peptidoglycan zymogram analysis also revealed 26, 28, 36, and 66 kDa peptidoglycan-degrading enzymes. n-MVs were often contaminated with flagella and pili when isolated by traditional methods, and a new strategy using a linear isopycnic sucrose gradient was utilized. For better characterization, this was applied to a representative genomovar IIIa strain (C5424) and showed that n-MVs consisted of a subset of specific outer membrane and periplasmic proteins as well as lipopoly saccharide possessing only a putative minor O-side chain polymer. This finding suggests that certain components are selected by B. cepacia during n-MV formation, and since some are putative virulence factors, this property could help deliver the factors to tissue, thereby aiding infection.Key words: membrane vesicles, virulence factors, Burkholderia cepacia, genomovar.

Bibliography

Allan, N. D., Kooi, C., Sokol, P. A., & Beveridge, T. J. (2003). Putative virulence factors are released in association with membrane vesicles fromBurkholderia cepacia. Canadian Journal of Microbiology, 49(10), 613â624.

Authors 4

Nick D Allan (first)
Cora Kooi (additional)
Pamela A Sokol (additional)
Terry J Beveridge (additional)

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Dates

Type	When
Created	21 years, 8 months ago (Dec. 8, 2003, 9:34 a.m.)
Deposited	4 years, 2 months ago (June 13, 2021, 7:37 a.m.)
Indexed	2 months ago (July 2, 2025, 3:27 p.m.)
Issued	21 years, 11 months ago (Oct. 1, 2003)
Published	21 years, 11 months ago (Oct. 1, 2003)
Published Print	21 years, 11 months ago (Oct. 1, 2003)

Funders 0

None

BibTeX

@article{Allan_2003, title={Putative virulence factors are released in association with membrane vesicles fromBurkholderia cepacia}, volume={49}, ISSN={1480-3275}, url={http://dx.doi.org/10.1139/w03-078}, DOI={10.1139/w03-078}, number={10}, journal={Canadian Journal of Microbiology}, publisher={Canadian Science Publishing}, author={Allan, Nick D and Kooi, Cora and Sokol, Pamela A and Beveridge, Terry J}, year={2003}, month=oct, pages={613–624} }

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