DOI: 10.1139/o85-096. Poly(ADP-ribosyl)ation of chromatin: kinetics of relaxation and its effect on chromatin solubility

Poly(ADP-ribosyl)ation of chromatin: kinetics of relaxation and its effect on chromatin solubility

10.1139/o85-096

Crossref journal-article

Canadian Science Publishing

Canadian Journal of Biochemistry and Cell Biology (155)

Abstract

We have studied the kinetics of relaxation of poly(ADP-ribosyl)ated polynucleosomes produced by endogenous enzyme activity by comparing the generation of hyper(ADP-ribosyl)ated histone H1 and its effect on the chromatin structure as revealed by electron microscopy. A correlation can be established between the appearance of histone H1 modified forms and the localized relaxation of the chromatin. We have also noticed, in parallel, that poly(ADP-ribosyl)ated chromatin showed increased solubility in the presence of Mg2+ and 0.2 M NaCl. Electron microscopic examination of the solubilized chromatin produced by poly(ADP-ribosyl)ation shows polynucleosomes exhibiting more relaxed conformation, whereas an increasing amount of hyper(ADP-ribosyl)ated histone H1 is found in the pellet, as shown by acid–urea–polyacrylamide electrophoretic separation of histone extracts.

Bibliography

Frechette, A., Huletsky, A., Aubin, R. J., Murcia, G. de, Mandel, P., Lord, A., Grondin, G., & Poirier, G. G. (1985). Poly(ADP-ribosyl)ation of chromatin: kinetics of relaxation and its effect on chromatin solubility. Canadian Journal of Biochemistry and Cell Biology, 63(7), 764â773.

Authors 8

André Frechette (first)
Ann Huletsky (additional)
Rémy J. Aubin (additional)
Gilbert de Murcia (additional)
Paul Mandel (additional)
André Lord (additional)
Gilles Grondin (additional)
Guy G. Poirier (additional)

References 0 Referenced 13

None

Dates

Type	When
Created	15 years, 8 months ago (Dec. 23, 2009, 9:02 a.m.)
Deposited	5 years, 7 months ago (Jan. 5, 2020, 10:38 a.m.)
Indexed	1 year, 7 months ago (Jan. 10, 2024, 4:14 a.m.)
Issued	40 years, 1 month ago (July 1, 1985)
Published	40 years, 1 month ago (July 1, 1985)
Published Print	40 years, 1 month ago (July 1, 1985)

Funders 0

None

BibTeX

@article{Frechette_1985, title={Poly(ADP-ribosyl)ation of chromatin: kinetics of relaxation and its effect on chromatin solubility}, volume={63}, ISSN={0714-7511}, url={http://dx.doi.org/10.1139/o85-096}, DOI={10.1139/o85-096}, number={7}, journal={Canadian Journal of Biochemistry and Cell Biology}, publisher={Canadian Science Publishing}, author={Frechette, André and Huletsky, Ann and Aubin, Rémy J. and Murcia, Gilbert de and Mandel, Paul and Lord, André and Grondin, Gilles and Poirier, Guy G.}, year={1985}, month=jul, pages={764–773} }

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