The rickettsial agent Coxiella burneti was studied in cultured mouse L cells by use of the electron microscope. Rickettsiae gain entry to the host cell in an apparently passive manner through phagocytic activity by L cells. The L cells show a lysosomal response to the presence of rickettsiae, as determined by cytochemical tests for acid phosphatase and 5′-nucleotidase. Further, examination of C. burneti within lysosomes suggests that rickettsiae can be degraded by the host cell. Autoradiographic analyses using tritiated thymidine show that rickettsial DNA is largely restricted to the dense nucleoid region, and when such labeled rickettsiae are used to inoculate L cells, most of the label becomes localized in the host cell nucleus. The above information is discussed in terms of dynamic interactions between C. burneti and infected L cells.

Burton, P. R., Kordová, N., & Paretsky, D. (1971). Electron microscopic studies of the rickettsia Coxiella burnetii: entry, lysosomal response, and fate of rickettsial DNA in L-cells. Canadian Journal of Microbiology, 17(2), 143–150.