DOI: 10.1139/b95-181. Particle regularity on thylakoid fracture faces is influenced by storage conditions

Particle regularity on thylakoid fracture faces is influenced by storage conditions

10.1139/b95-181

Crossref journal-article

Canadian Science Publishing

Canadian Journal of Botany (155)

Abstract

Specific temperature, storage times, and medium composition enable initiation of regular arrays of intramembranous particles on the exoplasmic fracture face during prolonged storage of isolated chloroplasts at 4 °C, producing about 2 – 10 regular arrays with 2 – 30 particles in each array, with a period of about 36 nm, oriented in 1 – 4 directions. The particle sizes do not change throughout the time of storage (1 – 4 weeks). The second type of particle regularity arises during prolonged storage of chloroplasts in greater than 1 M sucrose at −18 °C. Rounded areas of small particles tightly packed into paracrystalline arrays are found among less densely packed particles. The density of small particles is 4700 particles/μm2, and the mean size is 11 nm, whereas the particle density of the background is 1600 particles/μm2with a mean particle size of 13 nm compared with 1200 particles/μm2and mean size 16 nm in fresh chloroplasts. Based on the reduction of particle sizes and manner of packing on the fracture face, it is proposed that the small particles are a light-harvesting complex, separate from photosystem II and aggregated into paracrystalline arrays. The thylakoid lipids may participate in formation of particle regularity. Key words: thylakoid membrane, freeze fracture, particle regularity, low temperatures.

Bibliography

Semenova, G. A. (1995). Particle regularity on thylakoid fracture faces is influenced by storage conditions. Canadian Journal of Botany, 73(10), 1676â1682.

Authors 1

Galina A. Semenova (first)

References 13 Referenced 16

10.1016/0005-2736(80)90547-7 / Biochim. Biophys. Acta by Armond P.A. (1980)
10.1104/pp.57.5.681 / Plant Physiol. by Garber M.P. (1976)
10.1083/jcb.96.5.1414 / J. Cell Biol. by Kuhlbrandt W. (1983)
10.1083/jcb.84.1.40 / J. Cell Biol. by McDonnel A (1980)
10.1006/jmbi.1993.1591 / J. Mol. Biol. by Nussberger S. (1993)
10.1242/jcs.5.1.299 / J. Cell Sci. by Park R.B. (1969)
{'key': 'p_7/p_7_1', 'first-page': '67', 'volume': '37', 'author': 'Roussel Ch.', 'year': '1980', 'journal-title': 'Biol. Cell.'} / Biol. Cell. by Roussel Ch. (1980)
{'key': 'p_8/p_8_1', 'first-page': '50', 'volume': '18', 'author': 'Semenova G.A.', 'year': '1984', 'journal-title': 'Photosynthetica'} / Photosynthetica by Semenova G.A. (1984)
10.1083/jcb.91.1.113 / J. Cell Biol. by Siegel C.O. (1981)
10.1007/BF02906109 / Carlsberg Res. Commun. by Simpson D.J. (1978)
10.1007/BF02906493 / Carlsberg Res. Commun. by Simpson D.J. (1979)
10.1016/0005-2728(75)90153-X / Biochim. Biophys. Acta by Staehelin L.A. (1975)
{'key': 'p_13/p_13_1', 'first-page': '1', 'author': 'Staehelin L.A.', 'year': '1986', 'journal-title': 'Photosynthesis 111: photosynthetic membranes and light-harvesting systems. Edited by L.A. Staehelin and C.J. Arntzen. Springer-Verlag, Berlin.'} / Photosynthesis 111: photosynthetic membranes and light-harvesting systems. Edited by L.A. Staehelin and C.J. Arntzen. Springer-Verlag, Berlin. by Staehelin L.A. (1986)

Dates

Type	When
Created	17 years, 6 months ago (Feb. 25, 2008, 10:56 a.m.)
Deposited	4 years ago (Sept. 4, 2021, 1:51 p.m.)
Indexed	4 weeks, 2 days ago (Aug. 6, 2025, 9:35 a.m.)
Issued	29 years, 11 months ago (Oct. 1, 1995)
Published	29 years, 11 months ago (Oct. 1, 1995)
Published Print	29 years, 11 months ago (Oct. 1, 1995)

Funders 0

None

BibTeX

@article{Semenova_1995, title={Particle regularity on thylakoid fracture faces is influenced by storage conditions}, volume={73}, ISSN={0008-4026}, url={http://dx.doi.org/10.1139/b95-181}, DOI={10.1139/b95-181}, number={10}, journal={Canadian Journal of Botany}, publisher={Canadian Science Publishing}, author={Semenova, Galina A.}, year={1995}, month=oct, pages={1676–1682} }

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  "abstract": "<jats:p>Specific temperature, storage times, and medium composition enable initiation of regular arrays of intramembranous particles on the exoplasmic fracture face during prolonged storage of isolated chloroplasts at 4 \u00b0C, producing about 2 \u2013 10 regular arrays with 2 \u2013 30 particles in each array, with a period of about 36\u2002nm, oriented in 1 \u2013 4 directions. The particle sizes do not change throughout the time of storage (1 \u2013 4 weeks). The second type of particle regularity arises during prolonged storage of chloroplasts in greater than 1\u2002M sucrose at \u221218 \u00b0C. Rounded areas of small particles tightly packed into paracrystalline arrays are found among less densely packed particles. The density of small particles is 4700 particles/\u03bcm<jats:sup>2</jats:sup>, and the mean size is 11\u2002nm, whereas the particle density of the background is 1600 particles/\u03bcm<jats:sup>2</jats:sup>with a mean particle size of 13\u2002nm compared with 1200 particles/\u03bcm<jats:sup>2</jats:sup>and mean size 16\u2002nm in fresh chloroplasts. Based on the reduction of particle sizes and manner of packing on the fracture face, it is proposed that the small particles are a light-harvesting complex, separate from photosystem II and aggregated into paracrystalline arrays. The thylakoid lipids may participate in formation of particle regularity. Key words: thylakoid membrane, freeze fracture, particle regularity, low temperatures.</jats:p>",
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