DOI: 10.1136/jcp.44.7.610. Detection of Borrelia burgdorferi in patients with Lyme disease by the polymerase chain reaction.

Detection of Borrelia burgdorferi in patients with Lyme disease by the polymerase chain reaction.

10.1136/jcp.44.7.610

Crossref journal-article

BMJ

Journal of Clinical Pathology (239)

Abstract

Borrelia burgdorferi, the causative agent of Lyme disease, was detected in patients' serum by DNA amplification using the polymerase chain reaction (PCR). B burgdorferi was pelleted from serum samples by centrifugation (10,000 x g for 10 minutes) and lysed by treatment with ammonium hydroxide (100 degrees C for 15 minutes). Two pairs of "nested" PCR primers complementary to the gene encoding a major outer surface protein (OSP A) of B burgdorferi were used in DNA amplification under standard PCR conditions (Perkin-Elmer Cetus). Two out of five patients with erythema migrans, the characteristic primary skin lesion associated with early Lyme disease, were positive by the PCR. This method could form the basis of a useful routine laboratory test in those cases of early Lyme disease where conventional serological testing commonly yields equivocal or false negative results.

Bibliography

Guy, E. C., & Stanek, G. (1991). Detection of Borrelia burgdorferi in patients with Lyme disease by the polymerase chain reaction. Journal of Clinical Pathology, 44(7), 610â611.

Authors 2

E C Guy (first)
G Stanek (additional)

References 6 Referenced 195

Steere AC, Hutchinson GJ, Rahn DW, et al. Treatment of the early manifestations of Lyme disease. Ann Intern Med 1983;99:22-6. (10.7326/0003-4819-99-1-22) / Ann Intern Med / Treatment of the early manifestations of Lyme disease by Steere, A.C.; Hutchinson, G.J.; Rahn, D.W. (1983)
Bergstrom S, Bundoc VG, Barbour AG. Molecular analysis ofthe linear plasmid-encoded major outer surface proteins OspA and OspB of the Lyme disease spirochaete Borrelia burgdorferi. Mol Microbiol 1989;3:479-86. (10.1111/j.1365-2958.1989.tb00194.x) / Mol Microbiol / Molecular analysis ofthe linear plasmid-encoded major outer surface proteins OspA and OspB of the Lyme disease spirochaete Borrelia burgdorferi by Bergstrom, S.; Bundoc, V.G.; Barbour, A.G. (1989)
Benach JL, Bosler EM, Hanrahan JP, et al. Spirochetes isolated from the blood of two patients with Lyme disease. N Engl J Med 1983;308:740-2. (10.1056/NEJM198303313081302) / N Engl J Med / Spirochetes isolated from the blood of two patients with Lyme disease by Benach, J.L.; Bosler, E.M.; Hanrahan, J.P. (1983)
Garcia-Monco JC, Fernandez-Villar B, Benach JL. Adherence of the Lyme disease spirochete to glial cells and cells of glial origin. J Infect Dis 1989;160:497-506. (10.1093/infdis/160.3.497) / J Infect Dis / Adherence of the Lyme disease spirochete to glial cells and cells of glial origin by Garcia-Monco, J.C.; Fernandez-Villar, B.; Benach, J.L. (1989)
Thomas DD, Comstock LE. Interaction of Lyme disease spirochetes with cultured eucaryotic cells. Infect Immun 1989;57: 1324-6. (10.1128/IAI.57.4.1324-1326.1989) / Infect Immun / Interaction of Lyme disease spirochetes with cultured eucaryotic cells by Thomas, D.D.; Comstock, L.E. (1989)
Szczepanski A, Furie MB, Benach JL, et al. Interaction between Borrelia burgdorferi and endothelium in vitro. J Clin Invest 1990;85:1637-47. (10.1172/JCI114615) / J Clin Invest / Interaction between Borrelia burgdorferi and endothelium in vitro by Szczepanski, A.; Furie, M.B.; Benach, J.L. (1990)

Dates

Type	When
Created	18 years, 2 months ago (June 9, 2007, 12:50 a.m.)
Deposited	1 year, 3 months ago (May 31, 2024, 6:36 p.m.)
Indexed	6 days, 21 hours ago (Aug. 30, 2025, 12:26 p.m.)
Issued	34 years, 2 months ago (July 1, 1991)
Published	34 years, 2 months ago (July 1, 1991)
Published Online	34 years, 2 months ago (July 1, 1991)
Published Print	34 years, 2 months ago (July 1, 1991)

Funders 0

None

BibTeX

@article{Guy_1991, title={Detection of Borrelia burgdorferi in patients with Lyme disease by the polymerase chain reaction.}, volume={44}, ISSN={1472-4146}, url={http://dx.doi.org/10.1136/jcp.44.7.610}, DOI={10.1136/jcp.44.7.610}, number={7}, journal={Journal of Clinical Pathology}, publisher={BMJ}, author={Guy, E C and Stanek, G}, year={1991}, month=jul, pages={610–611} }

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