Ber-EP4: new monoclonal antibody which distinguishes epithelia from mesothelial.
10.1136/jcp.43.3.213
Crossref journal-article
BMJ
Journal of Clinical Pathology (239)
Abstract

A new monoclonal antibody, Ber-EP4, directed against a partially formol resistant epitope on the protein moiety of two 34 kilodalton and 39 kilodalton glycopolypeptides on human epithelial cells is described. Immunostaining of a wide range of normal and neoplastic human tissues and cell lines showed that all carcinomas and all non-neoplastic epithelial cells, except hepatocytes, parietal cells, and apical cell layers in squamous epithelia, homogeneously expressed Ber-EP4 antigen. As Ber-EP4 does not detect any normal or neoplastic non-epithelial cells, this antibody might prove valuable for the differentiation of the following (i) non-epithelial tumours from undifferentiated carcinomas; (ii) hepatocytes from bile duct cells in certain liver diseases; (iii) mesothelial cells from carcinoma cells in lung biopsy specimens; and (iv) reactive mesothelial cells from carcinoma cells in smears of serous effusions.

Bibliography

Latza, U., Niedobitek, G., Schwarting, R., Nekarda, H., & Stein, H. (1990). Ber-EP4: new monoclonal antibody which distinguishes epithelia from mesothelial. Journal of Clinical Pathology, 43(3), 213–219.

Authors 5
  1. U Latza (first)
  2. G Niedobitek (additional)
  3. R Schwarting (additional)
  4. H Nekarda (additional)
  5. H Stein (additional)
References 41 Referenced 311
  1. Gatter KC, Alcock C, Heryet A, Mason DY. Clinical importance of analysing malignant tumours of uncertain origin with immunohistological techniques. Lancet 1985;i: 1302-5. / Lancet / Clinical importance of analysing malignant tumours of uncertain origin with immunohistological techniques by Gatter, K.C.; Alcock, C.; Heryet, A.; Mason, D.Y. (1985)
  2. Damianow I, Knowles BB. Biology of disease: monoclonal antibodies and tumor-associated antigens. Lab Invest 1983;48:510-25. / Lab Invest / Biology of disease: monoclonal antibodies and tumor-associated antigens by Damianow, I.; Knowles, B.B. (1983)
  3. Gatter KC, Abdulaziz Z, Beverly P, et al. Use of monoclonal antibodies for the histopathological diagnosis of human malignancy. J Clin Pathol 1982;35:1253-67. (10.1136/jcp.35.11.1253) / J Clin Pathol / Use of monoclonal antibodies for the histopathological diagnosis of human malignancy by Gatter, K.C.; Abdulaziz, Z.; Beverly, P. (1982)
  4. Lauder I, Holland D, Mason DY, Gowland G, Cunliffe WJ. Identification of large cell undifferentiated tumors in lymph nodes using leukocyte common and keratin antibodies. Histopathology 1984;8:259-72. (10.1111/j.1365-2559.1984.tb02340.x) / Histopathology / Identification of large cell undifferentiated tumors in lymph nodes using leukocyte common and keratin antibodies by Lauder, I.; Holland, D.; Mason, D.Y.; Gowland, G.; Cunliffe, W.J. (1984)
  5. Cooper D, Schermer A, Sun T-T. Biology of disease: classification of human epithelia and their neoplasms using monoclonal antibodies to keratins: strategies, applications, and limitations. Lab Invest 1985;52:243-56. / Lab Invest / Biology of disease: classification of human epithelia and their neoplasms using monoclonal antibodies to keratins: strategies, applications, and limitations by Cooper, D.; Schermer, A.; Sun, T.-T. (1985)
  6. Osbom M, Weber K. Tumor diagnosis by intermediate filament typing: a novel tool for surgical pathology. Lab Invest 1983;48:372-94. / Lab Invest / Tumor diagnosis by intermediate filament typing: a novel tool for surgical pathology by Osbom, M.; Weber, K. (1983)
  7. Kasper M, Karsten U. Coexpression of cytokeratin and vimentin in Rathke's cysts of human pituitary gland. Cell Tissue Res 1988;253:419-24. / Cell Tissue Res / Coexpression of cytokeratin and vimentin in Rathke's cysts of human pituitary gland by Kasper, M.; Karsten, U. (1988)
  8. Grone H-J, Weber K, Grone E, Helmchen U, Osborn M. Coexpression of keratin and vimentin in damaged and regenerating tubular epithelia of the kidney. Am J Pathol 1987;129: 1-8. / Am J Pathol / Coexpression of keratin and vimentin in damaged and regenerating tubular epithelia of the kidney by Grone, H.-J.; Weber, K.; Grone, E.; Helmchen, U.; Osborn, M. (1987)
  9. Henzen-Logmans SC, Mullink H, Ramackers FCS, Tadema T, Meijer CJLM. Expression of cytokeratins and vimentin in epithelial cells of normal and pathological thyroid tissue. Virchows Arch (Cell Pathol) 1987;410: 347-54. / Virchows Arch (Cell Pathol) / Expression of cytokeratins and vimentin in epithelial cells of normal and pathological thyroid tissue by Henzen-Logmans, S.C.; Mullink, H.; Ramackers, F.C.S.; Tadema, T.; Meijer, C.J.L.M. (1987)
  10. Domagala W, Weber K, Osborn M. Diagnostic significance of coexpression of intermediate filaments in fine needle aspirates of human tumors. Acta Cytol 1988;32:49-59. / Acta Cytol / Diagnostic significance of coexpression of intermediate filaments in fine needle aspirates of human tumors by Domagala, W.; Weber, K.; Osborn, M. (1988)
  11. Milani S, Herbst H, Schuppan D, et al. Vimentin expression in proliferating bile duct epithelia. Virchow Arch (Pathol Anat) 1989;415:237-42. (10.1007/BF00724910) / Virchow Arch (Pathol Anat) / Vimentin expression in proliferating bile duct epithelia by Milani, S.; Herbst, H.; Schuppan, D. (1989)
  12. Wagner C, Breuer H. Biochemical and immunochemical aspects of carcinoembryonic antigen (CEA) and CEA related antigens: current status and future perspectives. J Clin Chem Clin Biochem 1982;20:705-12. / J Clin Chem Clin Biochem / Biochemical and immunochemical aspects of carcinoembryonic antigen (CEA) and CEA related antigens: current status and future perspectives by Wagner, C.; Breuer, H. (1982)
  13. Cordell J, Richardson TC, Pulford KAF et al. Production of monoclonal antibodies against human epithelial membrane antigen for use in diagnostic immunocytochemistry. Br J Cancer 1985;52:347-54. (10.1038/bjc.1985.200) / Br J Cancer / Production of monoclonal antibodies against human epithelial membrane antigen for use in diagnostic immunocytochemistry by Cordell, J.; Richardson, T.C.; Pulford, K.A.F. (1985)
  14. Edwards PAW. Heterogenous expression of cell-surface antigens in normal epithelia and their tumours, revealed by monoclonal antibodies. Br J Cancer 1985;51:149-60. (10.1038/bjc.1985.24) / Br J Cancer / Heterogenous expression of cell-surface antigens in normal epithelia and their tumours, revealed by monoclonal antibodies by Edwards, P.A.W. (1985)
  15. Delsol G, Stein H, Pulford KAF, Gatter KC, Erber WN, Zinne K. Human lymphoid cells express epithelial membrane antigen. Lancet 1984;ii:1 124-8. / Lancet / Human lymphoid cells express epithelial membrane antigen by Delsol, G.; Stein, H.; Pulford, K.A.F.; Gatter, K.C.; Erber, W.N.; Zinne, K. (1984)
  16. Gosh AK, Gatter KC, Dunnhill MS, Mason DY. Immunohistological staining of reactive mesothelium, mesothelioma, and lung carcinoma with a panel of monoclonal antibodies. J Clin Pathol 1987;40:19-25. (10.1136/jcp.40.1.19) / J Clin Pathol / Immunohistological staining of reactive mesothelium, mesothelioma, and lung carcinoma with a panel of monoclonal antibodies by Gosh, A.K.; Gatter, K.C.; Dunnhill, M.S.; Mason, D.Y. (1987)
  17. Momburg F, Moldenhauer G, Hammerling GJ, Moller P. Immunohistochemical study of the expression of a M 34,000 human epithelium-specific surface glycoprotein in normal and malignant tissues. Cancer Res 1987;47: 2883-91. / Cancer Res / Immunohistochemical study of the expression of a M 34,000 human epithelium-specific surface glycoprotein in normal and malignant tissues by Momburg, F.; Moldenhauer, G.; Hammerling, G.J.; Moller, P. (1987)
  18. Moldenhauer G, Momburg F, Moller P, Schwartz R, Hammerling GJ. Epithelium-specific surface glycoprotein of M, 34,000 is a widely distributed human carcinoma marker. Br J Cancer 1987;56:714-21. (10.1038/bjc.1987.276) / Br J Cancer / Epithelium-specific surface glycoprotein of M, 34,000 is a widely distributed human carcinoma marker by Moldenhauer, G.; Momburg, F.; Moller, P.; Schwartz, R.; Hammerling, G.J. (1987)
  19. Dippold WG, Klingel R, Bernhard H, Dienes H-P, Knuth A, Meyer zum Buschenfelde K-H. Epithelial cell marker on gastrointestinal tumors and in human secretions defined by a monoclonal antibody. Cancer Res 1987;47:2092-7. / Cancer Res / Epithelial cell marker on gastrointestinal tumors and in human secretions defined by a monoclonal antibody by Dippold, W.G.; Klingel, R.; Bernhard, H.; Dienes, H.-P.; Knuth, A.; Meyer zum Buschenfelde, K.-H. (1987)
  20. Jones DB, Scott CS, Wright DH et al. Phenotypic analysis of an established cell line derived from a patient with Hodgkin's disease (HD). Haematol Oncol 1985;3:133-45. (10.1002/hon.2900030205) / Haematol Oncol / Phenotypic analysis of an established cell line derived from a patient with Hodgkin's disease (HD) by Jones, D.B.; Scott, C.S.; Wright, D.H. (1985)
  21. Kamesaki H, Fukuhara S, Tatsumi E, et al. Cytochemical, immunological, chromosomal and molecular genetic analysis of a novel cell line derived from Hodgkin's disease. Blood 1986;68:285. (10.1182/blood.V68.1.285.285) / Blood / chromosomal and molecular genetic analysis of a novel cell line derived from Hodgkin's disease by Kamesaki, H.; Fukuhara, S.; Tatsumi, E. (1986)
  22. Diehl V, Kirchner HH, Schaadt M, Fonatsch C, Stein H, Gerdes J, Boie C. Hodgkin's disease: establishment of four in vitro cell lines. J Cancer Res Clin Oncol 1981;101: 111-24. (10.1007/BF00405072) / J Cancer Res Clin Oncol / Hodgkin's disease: establishment of four in vitro cell lines by Diehl, V.; Kirchner, H.H.; Schaadt, M.; Fonatsch, C.; Stein, H.; Gerdes, J.; Boie, C. (1981)
  23. Diehl V, Kirchner HH, Burrichter H, et al. Characteristics of Hodgkin's disease derived cell lines. Cancer Treat Rep 1982;66:61 5-32. / Cancer Treat Rep / Characteristics of Hodgkin's disease derived cell lines by Diehl, V.; Kirchner, H.H.; Burrichter, H. (1982)
  24. Cordell J, Falini B, Erber WW, et al. Immunoenzymatic labelling of monoclonal antibodies with immune complexes of alkaline phosphatase and monoclonal antialkaline phosphatase (APAAP) complexes. J Histochem Cytochem 1984;32:219-29. (10.1177/32.2.6198355) / J Histochem Cytochem / Immunoenzymatic labelling of monoclonal antibodies with immune complexes of alkaline phosphatase and monoclonal antialkaline phosphatase (APAAP) complexes by Cordell, J.; Falini, B.; Erber, W.W. (1984)
  25. Stein H, Gatter KC, Asbahr H, Mason DY. Methods in Laboratory Investigation. Use of freeze-dried paraffinembedded sections for immunohistologic staining with monoclonal antibodies. Lab Invest 1985;52:676-83. / Lab Invest / Methods in Laboratory Investigation. Use of freeze-dried paraffinembedded sections for immunohistologic staining with monoclonal antibodies by Stein, H.; Gatter, K.C.; Asbahr, H.; Mason, D.Y. (1985)
  26. Stein H, Gerdes J, Schwab U, et al. Identification of Hodgkin and Steinberg-Reed cells as a unique cell type derived from a newly detected small cell population. Int J Cancer 1980;30:445-59. (10.1002/ijc.2910300411) / Int J Cancer / Identification of Hodgkin and Steinberg-Reed cells as a unique cell type derived from a newly detected small cell population by Stein, H.; Gerdes, J.; Schwab, U. (1980)
  27. Kohler G, Milstein C. Continous culture of fused cells secreting antibody of predefined specificity. Nature 1975; 32:495-7. (10.1038/256495a0) / Nature / Continous culture of fused cells secreting antibody of predefined specificity by Kohler, G.; Milstein, C. (1975)
  28. Oi VT, Herzenberg LA. Immunoglobulin-producing hybrid cell lines. In: Mishell BB, Shiigi SM, eds. Selected methods in cellular immunology. San Francisco: Freeman, 1980:351-72. / methods in cellular immunology / Immunoglobulin-producing hybrid cell lines by Oi, V.T.; Herzenberg, L.A. (1980)
  29. Thorell Y, Johansson BG. Enzymatic iodination ofpolypeptides with "'5J to high specific activity. Biochem Biophys Acta 1971;251:363. / Biochem Biophys Acta / Enzymatic iodination ofpolypeptides with "'5J to high specific activity by Thorell, Y.; Johansson, B.G. (1971)
  30. Wano Y, Uchiyama T, Fukuki K, Maeda M, Uchino H, Yodo J. Characterization of human interleukin 2 receptor (Tac antigen) in normal and leukemic T cells: coexpression of normal and aberrant receptors on Hut-102 cells. J Immunol 1984;132:3005-10. / J Immunol / Characterization of human interleukin 2 receptor (Tac antigen) in normal and leukemic T cells: coexpression of normal and aberrant receptors on Hut-102 cells by Wano, Y.; Uchiyama, T.; Fukuki, K.; Maeda, M.; Uchino, H.; Yodo, J. (1984)
  31. Schwarting R, Stein H, Chang Yi Wang. The monoclonal antibodies aS-HCLI (aLeu-14) and aS-HCL3 (aLeu- M5) allow the diagnosis of hairy cell leukemia. Blood 1985;65:974. (10.1182/blood.V65.4.974.974) / Blood / The monoclonal antibodies aS-HCLI (aLeu-14) and aS-HCL3 (aLeu- M5) allow the diagnosis of hairy cell leukemia by Schwarting, R.; Stein, H.; Wang, Chang Yi (1985)
  32. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970;277:680. (10.1038/227680a0) / Nature / Cleavage of structural proteins during the assembly of the head of bacteriophage T4 by Laemmli, U.K. (1970)
  33. Schwarting R, Gedes J, Stein H. BER-H2: a new monoclonal antibody of the Ki-I family for the detection of Hodgkin's disease in formalin-fixed tissue sections (A2.13). In: McMichael AJ, ed. Leucocyte typing III, white cell differentiation antigens. Oxford: Oxford University Press, 1987:574-5. / typing III, white cell differentiation antigens / BER-H2: a new monoclonal antibody of the Ki-I family for the detection of Hodgkin's disease in formalin-fixed tissue sections (A2.13) by Schwarting, R.; Gedes, J.; Stein, H. (1987)
  34. Thotakura NR, Bahl OP. Enzymatic deglycosylation of glycoproteins. Methods in Enzymology 1985;138:350-9. (10.1016/0076-6879(87)38030-9) / Methods in Enzymology / Enzymatic deglycosylation of glycoproteins by Thotakura, N.R.; Bahl, O.P. (1985)
  35. Woodward MP, Young WW, Bloodgood RA. Detection of monoclonal antibodies specific for carbohydrate epitopes using periodate oxidation. J Immunol Methods 1985; 78:143-53. (10.1016/0022-1759(85)90337-0) / J Immunol Methods / Detection of monoclonal antibodies specific for carbohydrate epitopes using periodate oxidation by Woodward, M.P.; Young, W.W.; Bloodgood, R.A. (1985)
  36. Stefani M, DeMartino C, Zamboni L. Fixation ofejaculated spermatazoa for electron microscopy. Nature 1967;216:173-4. (10.1038/216173a0) / Nature / Fixation ofejaculated spermatazoa for electron microscopy by Stefani, M.; DeMartino, C.; Zamboni, L. (1967)
  37. Cordon-Cardo C, Mattes MJ, Melamed MR, Lewis JL, Jr, Old LJ, Lloyd KO. Immunopathologic analysis ofa panel of mouse monoclonal antibodies reacting with human ovarian carcinomas and other human tumors. Int J Gynecol Pathol 1985;4:121-30. (10.1097/00004347-198506000-00004) / Int J Gynecol Pathol / Immunopathologic analysis ofa panel of mouse monoclonal antibodies reacting with human ovarian carcinomas and other human tumors by Cordon-Cardo, C.; Mattes, M.J.; Melamed, M.R.; Lewis, Jr, J.L.; Old, L.J.; Lloyd, K.O. (1985)
  38. Lozzio CB, Lozzio BB. Human chronic myelogenous leukemia cell line with positive Philadelphia chromosome. Blood 1975;45:321-34. (10.1182/blood.V45.3.321.321) / Blood / Human chronic myelogenous leukemia cell line with positive Philadelphia chromosome by Lozzio, C.B.; Lozzio, B.B. (1975)
  39. Zauli D, Gobbi M, Crespi C, et al. Vimentin and keratin intermediate filaments expression by K562 leukemic cell line. Leuk Res 1986;10:29-33. (10.1016/0145-2126(86)90102-5) / Leuk Res / Vimentin and keratin intermediate filaments expression by K562 leukemic cell line by Zauli, D.; Gobbi, M.; Crespi, C. (1986)
  40. Van Eyken P, Sciot R, Van Damme B, de Wolf-Peeters C, Desmet VJ. Keratin immunohistochemistry in normal human liver. Cytokeratin pattem of hepatocytes, bile ducts and acinar gradient. Virchows Arch (Cell Pathol) 1987;412:63-72. / Virchows Arch (Cell Pathol) / Keratin immunohistochemistry in normal human liver. Cytokeratin pattem of hepatocytes, bile ducts and acinar gradient by P, Van Eyken; R, Sciot; B, Van Damme; de Wolf-Peeters C; VJ, Desmet (1987)
  41. Warnock ML, Stoloff A, Thor A. Differentiation of adenocarcinoma of the lung from mesothelioma: periodic acidSchiff, monoclonal antibodies B72.3, and Leu Ml. Am J Pathol 1988;133:30-8. / Leu Ml. Am J Pathol / Differentiation of adenocarcinoma of the lung from mesothelioma: periodic acidSchiff, monoclonal antibodies B72.3, and by Warnock, M.L.; Stoloff, A.; Thor, A. (1988)
Dates
Type When
Created 18 years, 2 months ago (June 9, 2007, 12:50 a.m.)
Deposited 1 year, 3 months ago (May 31, 2024, 6:27 p.m.)
Indexed 3 days, 4 hours ago (Sept. 4, 2025, 10:07 a.m.)
Issued 35 years, 6 months ago (March 1, 1990)
Published 35 years, 6 months ago (March 1, 1990)
Published Online 35 years, 6 months ago (March 1, 1990)
Published Print 35 years, 6 months ago (March 1, 1990)
Funders 0

None

@article{Latza_1990, title={Ber-EP4: new monoclonal antibody which distinguishes epithelia from mesothelial.}, volume={43}, ISSN={1472-4146}, url={http://dx.doi.org/10.1136/jcp.43.3.213}, DOI={10.1136/jcp.43.3.213}, number={3}, journal={Journal of Clinical Pathology}, publisher={BMJ}, author={Latza, U and Niedobitek, G and Schwarting, R and Nekarda, H and Stein, H}, year={1990}, month=mar, pages={213–219} }