DOI: 10.1136/jcp.27.1.14. The demonstration of plasma cells and other immunoglobulin-containing cells in formalin-fixed, paraffin-embedded tissues using peroxidase-labelled antibody

The demonstration of plasma cells and other immunoglobulin-containing cells in formalin-fixed, paraffin-embedded tissues using peroxidase-labelled antibody

10.1136/jcp.27.1.14

Crossref journal-article

BMJ

Journal of Clinical Pathology (239)

Abstract

A method is described for the demonstration of specific immunoglobulin in plasma cells and other lymphoid cells in sections taken from routine surgical histology specimens which have been formalin fixed and paraffin embedded.An indirect sandwich technique was employed using specific rabbit antihuman immunoglobulin antisera (anti-K, L, G, A, and M) and a swine antirabbit serum Ig G, conjugated with horseradish peroxidase. The presence of plasma cells was revealed by staining the tissue-bound peroxidase-labelled antibody, having previously stained the endogenous peroxidase a contrasting colour.It was possible to demonstrate clearly immunoglobulin in the plasma cells of tissues processed and embedded several years previously.Some of the potential uses of the method are discussed.

Bibliography

Taylor, C. R., & Burns, J. (1974). The demonstration of plasma cells and other immunoglobulin-containing cells in formalin-fixed, paraffin-embedded tissues using peroxidase-labelled antibody. Journal of Clinical Pathology, 27(1), 14â20.

Authors 2

C. R. Taylor (first)
J. Burns (additional)

References 16 Referenced 293

Avrameas, S. (1969). Indirect immunoenzyme techniques for the intracellular detection of antigens. Immunochemistry, 6, 825-831. (10.1016/0019-2791(69)90288-2) / Immunochemistry / Indirect immunoenzyme techniques for the intracellular detection of antigens by Avrameas, S. (1969)
Avrameas, S., and Ternynck, T. (1969). The cross-linking of proteins with glutaraldehyde and its use for the preparation of immunoadsorbents. Immunochemistry, 6, 53-66. (10.1016/0019-2791(69)90178-5) / Immunochemistry / The cross-linking of proteins with glutaraldehyde and its use for the preparation of immunoadsorbents by Avrameas, S.; Ternynck, T. (1969)
Bosman, C., and Feldman, J. D. (1970). Heterogeneity and homogeneity of immunoglobulin-forming cells. Lab. Invest., 22, 309-317. / Lab. Invest / Heterogeneity and homogeneity of immunoglobulin-forming cells by Bosman, C.; Feldman, J.D. (1970)
Bosman, C., Feldman, J. D., and Pick, E. (1969). Heterogeneity of antibody-forming cells: an electron microscopic analysis. J. exp. Med., 129, 1029-1044. (10.1084/jem.129.5.1029) / J. exp. Med / Heterogeneity of antibody-forming cells: an electron microscopic analysis by Bosman, C.; Feldman, J.D.; Pick, E. (1969)
Coons, A. H. (1956). Histochemistry with labelled antibody. Int. Rev. Cytol., 5, 1-23. (10.1016/S0074-7696(08)62565-6) / Int. Rev. Cytol / Histochemistry with labelled antibody by Coons, A.H. (1956)
Coons, A. H., Creech, H. J., and Jones, R. N. (1941). Immunological properties of an antibody containing a fluorescent group. Proc. Soc. exp. Biol. (N. Y.) 47, 200-202. (10.3181/00379727-47-13084P) / Proc. Soc. exp. Biol. (N. Y.) / Immunological properties of an antibody containing a fluorescent group by Coons, A.H.; Creech, H.J.; Jones, R.N. (1941)
Davey, F. R., and Busch, G. J. (1970). Immunohistochemistry of glomerulonephritis using horseradish peroxidase and fluorescein-labeled antibody: a comparison of two techniques. Amer. J. clin. Path., 53, 531-536. (10.1093/ajcp/53.4.531) / Amer. J. clin. Path / Immunohistochemistry of glomerulonephritis using horseradish peroxidase and fluorescein-labeled antibody: a comparison of two techniques by Davey, F.R.; Busch, G.J. (1970)
Fagreus, A. (1948). Antibody production in relation tothe development of plasma cells: in vivo and in vitro experiments. Acta med. scand., 130, Suppl. 204, Feldman, J. D. (1972). The morphology -of plasma cells. In Hematology, edited by W. J. Williams et al, pp. 771-781. McGraw-Hill New York. / Acta med. scand; The morphology -of plasma cells / Antibody production in relation tothe development of plasma cells: in vivo and in vitro experiments by Fagreus, A. (1948)
Graham, R. C., Jr., and Karnovsky, M. J. (1966). The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J. Histochem. Cytochem., 14, 291-302. (10.1177/14.4.291) / J. Histochem. Cytochem / The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique by Graham, Jr., R.C.; Karnovsky, M.J. (1966)
Holborow, E. J. Ed. (1970). Standardisation in Immunofluorescence. Blackwell, Oxford. / Standardisation in Immunofluorescence by Holborow, E.J. (1970)
Lie, J. T., Holley, K. E., Kampa, W. R., and Titus, J. L. (1971). New histochemical method for morphological diagnosis of early stages of myocardial ischaemia. Mayo Clin. Proc., 46, 319-327. / Mayo Clin. Proc / New histochemical method for morphological diagnosis of early stages of myocardial ischaemia by Lie, J.T.; Holley, K.E.; Kampa, W.R.; Titus, J.L. (1971)
Lillie, R. D. (1965). Histopathological Technic and Practical Histochemistry, 3rd. ed., p. 371. McGraw-Hill, London. Marschalk6, T. (1895a). Ueber die sog en annten. Plasmazellen, ein / Ueber die sog en annten / Histopathological Technic and Practical Histochemistry by Lillie, R.D. (1965)
Beitrag, zur Kenntniss der Herkunft der Entzundlichen Infiltrationszellen. Arch. Derm. Syph. (Berl.), 30, 241-282. Marschalk6, T. (1895b). A agynevezett plasmasejtekrol, adatok a lobos beszurod6s sejtjeinek eredetehez (The so-called plasma cells: contributions on the origin of the inflammatory percolation of cells). Orv. Hetil., 39, 36, 49 and 63.. Nakane, P. K. (1968). Simultaneous localization of multiple tissue antigens using the peroxidase-labeled antibody method: a study on pituitary glands of the rat. J. Histochem. Cytochem., 16, 557-560. / Arch. Derm. Syph. (Berl.); Simultaneous localization of multiple tissue antigens using the peroxidase-labeled antibody method: a study on pituitary glands of the rat / A agynevezett plasmasejtekrol, adatok a lobos beszurod6s sejtjeinek eredetehez (The so-called plasma cells: contributions on the origin of the inflammatory percolation of cells) by Beitrag, zur Kenntniss der Herkunft der Entzundlichen Infiltrationszellen (1895)
Nakane, P. K., and Pierce, G. B., Jr. (1966). Enzyme-labelled antibodies: preparation and application for the localization of antigens. J. Histochem. Cytochem., 14, 929-931. (10.1177/14.12.929) / J. Histochem. Cytochem / Enzyme-labelled antibodies: preparation and application for the localization of antigens by Nakane, P.K.; Pierce, Jr, G.B. (1966)
Petts, V., and Roitt, I. M. (1971). Peroxidase conjugates for demonstration of tissue antibodies: evaluation of the technique. Clin. exp. Immunol., 9, 407-418. / Clin. exp. Immunol / Peroxidase conjugates for demonstration of tissue antibodies: evaluation of the technique by Petts, V.; Roitt, I.M. (1971)
Sainte-Marie, G. (1962). A paraffin embedding technique for studies employing immunofluorescence. J. Histochem. Cytochem.. 10, 250-256. (10.1177/10.3.250) / J. Histochem. Cytochem / A paraffin embedding technique for studies employing immunofluorescence by Sainte-Marie, G. (1962)

Dates

Type	When
Created	18 years, 2 months ago (June 9, 2007, 12:50 a.m.)
Deposited	1 year, 3 months ago (May 31, 2024, 9:11 a.m.)
Indexed	2 days, 15 hours ago (Sept. 4, 2025, 10:24 a.m.)
Issued	51 years, 8 months ago (Jan. 1, 1974)
Published	51 years, 8 months ago (Jan. 1, 1974)
Published Online	51 years, 8 months ago (Jan. 1, 1974)
Published Print	51 years, 8 months ago (Jan. 1, 1974)

Funders 0

None

BibTeX

@article{Taylor_1974, title={The demonstration of plasma cells and other immunoglobulin-containing cells in formalin-fixed, paraffin-embedded tissues using peroxidase-labelled antibody}, volume={27}, ISSN={1472-4146}, url={http://dx.doi.org/10.1136/jcp.27.1.14}, DOI={10.1136/jcp.27.1.14}, number={1}, journal={Journal of Clinical Pathology}, publisher={BMJ}, author={Taylor, C. R. and Burns, J.}, year={1974}, month=jan, pages={14–20} }

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