DOI: 10.1135/cccc19800298. Immobilization of some hydrolases on polymethacrylate by the method of reactive esters

Immobilization of some hydrolases on polymethacrylate by the method of reactive esters

10.1135/cccc19800298

Crossref journal-article

Institute of Organic Chemistry & Biochemistry

Collection of Czechoslovak Chemical Communications (111)

Abstract

The immobilization of chymotrypsin (EC 3.4.4.5), papain (EC 3.4.4.10), α-amylase (EC 3.2.1.1), amyloglucosidase (EC 3.2.1.3), and of a technical pectolytic enzyme on various reactive polymethacrylate esters (Ostion KM) was examined and compared with the immobilization of the same enzymes on carboxymethyl-cellulose. There is no difference in the individual reactive esters (2,4-dinitrophenyl, N-hydroxysuccinimidyl, ethyl-1-hydroxyacetyl) when enzymes reacting fast (papain, chymotrypsin) are immobilized. The ethyl-1-hydroxyacetyl ester of the support is most suitable for slowly reacting enzymes. The reaction does not proceed at pH-values lower than 7.5. The specific activity of immobilized amylolytic and pectolytic enzymes decreases with the increasing pH because of inactivation of the enzyme.

Bibliography

KuÄera, J., & KmÃnkovÃ¡, M. (1980). Immobilization of some hydrolases on polymethacrylate by the method of reactive esters. Collection of Czechoslovak Chemical Communications, 45(1), 298â306.

Authors 2

Jiří Kučera (first)
Milena Kmínková (additional)

References 0 Referenced 3

None

Dates

Type	When
Created	11 years, 7 months ago (Jan. 25, 2014, 3:22 a.m.)
Deposited	6 years, 11 months ago (Sept. 9, 2018, 7:36 a.m.)
Indexed	3 years, 4 months ago (April 5, 2022, 12:52 p.m.)
Issued	45 years, 8 months ago (Jan. 1, 1980)
Published	45 years, 8 months ago (Jan. 1, 1980)
Published Online	45 years, 8 months ago (Jan. 1, 1980)
Published Print	45 years, 8 months ago (Jan. 1, 1980)

Funders 0

None

BibTeX

@article{Ku_era_1980, title={Immobilization of some hydrolases on polymethacrylate by the method of reactive esters}, volume={45}, ISSN={1212-6950}, url={http://dx.doi.org/10.1135/cccc19800298}, DOI={10.1135/cccc19800298}, number={1}, journal={Collection of Czechoslovak Chemical Communications}, publisher={Institute of Organic Chemistry & Biochemistry}, author={Kučera, Jiří and Kmínková, Milena}, year={1980}, pages={298–306} }

JSON

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