DOI: 10.1128/jvi.64.2.742-747.1990. Mutational analysis of the human immunodeficiency virus type 2 (HIV-2) genome in relation to HIV-1 and simian immunodeficiency virus SIV (AGM)

Mutational analysis of the human immunodeficiency virus type 2 (HIV-2) genome in relation to HIV-1 and simian immunodeficiency virus SIV (AGM)

10.1128/jvi.64.2.742-747.1990

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

We constructed an infectious molecular clone of the human immunodeficiency virus type 2 (HIV-2) and generated nine frameshift mutants corresponding to nine open reading frames identified so far. Three structural (gag, pol, env) and two regulative (tat, rev) gene mutants were not infectious, whereas vif, vpx, vpr, and nef genes were dispensable for infectivity. All of the mutants except env and rev were cytopathic in CD4+ human leukemia cells. In transfection assays, the expression of HIV-2 long terminal repeat was activated by infectious clones of HIV-1, HIV-2, and simian immunodeficiency virus from African green monkey but not by the tat mutants. However, an HIV-2 tat mutant could produce small amounts of virus proteins and particles in contrast to a rev mutant, which directed no detectable synthesis of virus proteins and virions.

Bibliography

Shibata, R., Miura, T., Hayami, M., Ogawa, K., Sakai, H., Kiyomasu, T., Ishimoto, A., & Adachi, A. (1990). Mutational analysis of the human immunodeficiency virus type 2 (HIV-2) genome in relation to HIV-1 and simian immunodeficiency virus SIV (AGM). Journal of Virology, 64(2), 742â747.

Authors 8

R Shibata (first)
T Miura (additional)
M Hayami (additional)
K Ogawa (additional)
H Sakai (additional)
T Kiyomasu (additional)
A Ishimoto (additional)
A Adachi (additional)

References 0 Referenced 92

None

Dates

Type	When
Created	5 years, 7 months ago (Jan. 6, 2020, 10:46 a.m.)
Deposited	3 years, 5 months ago (March 4, 2022, 6:52 p.m.)
Indexed	1 year ago (Aug. 4, 2024, 7:17 p.m.)
Issued	35 years, 7 months ago (Feb. 1, 1990)
Published	35 years, 7 months ago (Feb. 1, 1990)
Published Print	35 years, 7 months ago (Feb. 1, 1990)

Funders 0

None

BibTeX

@article{Shibata_1990, title={Mutational analysis of the human immunodeficiency virus type 2 (HIV-2) genome in relation to HIV-1 and simian immunodeficiency virus SIV (AGM)}, volume={64}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.64.2.742-747.1990}, DOI={10.1128/jvi.64.2.742-747.1990}, number={2}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Shibata, R and Miura, T and Hayami, M and Ogawa, K and Sakai, H and Kiyomasu, T and Ishimoto, A and Adachi, A}, year={1990}, month=feb, pages={742–747} }

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