DOI: 10.1128/jvi.63.12.5501-5504.1989. trans activation of human immunodeficiency virus type 1 is sequence specific for both the single-stranded bulge and loop of the trans-acting-responsive hairpin: a quantitative analysis

trans activation of human immunodeficiency virus type 1 is sequence specific for both the single-stranded bulge and loop of the trans-acting-responsive hairpin: a quantitative analysis

10.1128/jvi.63.12.5501-5504.1989

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

We have used site-directed mutagenesis to delineate sequence specific domains within the human immunodeficiency virus type 1 (HIV-1) trans-acting-responsive (TAR) RNA element that are required for trans activation by the viral Tat protein. Our data in part corroborate a recent report [S. Feng and E. C. Holland, Nature (London) 334:165-167, 1988] that five nucleotides within the loop (+29 to +33) of the TAR hairpin are important for trans activation. We, however, found no absolute requirement for the CUGGG loop sequence. Mutants with substitutions within the loop retained between 9 and 50% activity compared with the wild type. A second sequence, important for trans activation, was found in the 3-base bulge loop (+22 to +24) of the TAR hairpin. Cross-trans-activation studies of mutant HIV-1 TAR elements with the HIV-2 Tat protein suggest that a similar recognition event(s) forms the basis for trans activation of HIV-1 and HIV-2.

Bibliography

Berkhout, B., & Jeang, K. T. (1989). trans activation of human immunodeficiency virus type 1 is sequence specific for both the single-stranded bulge and loop of the trans-acting-responsive hairpin: a quantitative analysis. Journal of Virology, 63(12), 5501â5504.

Authors 2

B Berkhout (first)
K T Jeang (additional)

References 0 Referenced 166

None

Dates

Type	When
Created	5 years, 7 months ago (Jan. 6, 2020, 10:03 a.m.)
Deposited	3 years, 5 months ago (March 4, 2022, 6:27 p.m.)
Indexed	1 year, 1 month ago (July 31, 2024, 3:52 p.m.)
Issued	35 years, 9 months ago (Dec. 1, 1989)
Published	35 years, 9 months ago (Dec. 1, 1989)
Published Print	35 years, 9 months ago (Dec. 1, 1989)

Funders 0

None

BibTeX

@article{Berkhout_1989, title={trans activation of human immunodeficiency virus type 1 is sequence specific for both the single-stranded bulge and loop of the trans-acting-responsive hairpin: a quantitative analysis}, volume={63}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.63.12.5501-5504.1989}, DOI={10.1128/jvi.63.12.5501-5504.1989}, number={12}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Berkhout, B and Jeang, K T}, year={1989}, month=dec, pages={5501–5504} }

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