DOI: 10.1128/jvi.54.3.879-881.1985. The immediate-early enhancer element of herpes simplex virus type 1 can replace a regulatory region of the c-Ha-ras1 oncogene required for transformation

The immediate-early enhancer element of herpes simplex virus type 1 can replace a regulatory region of the c-Ha-ras1 oncogene required for transformation

10.1128/jvi.54.3.879-881.1985

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

A 0.8-kilobase SacI DNA fragment in the distal 5'-noncoding region of the c-Ha-ras1 oncogene hybridized to high guanine X cytosine sites of herpes simplex virus type 1 (HSV-1) DNA restriction fragments. Nucleotide sequence comparisons localized one of these sites to the intergenic region of HSV between the immediate-early genes coding for IEmRNA-3 and IEmRNA-4/5 that has enhancer-type activity. We tested the possibility that the HSV-1 enhancer and the upstream c-Ha-ras1 SacI fragment were functionally related by assaying for the capacity of recombinant plasmids in which the HSV-1 enhancer replaced the oncogene 0.8-kilobase SacI fragment to transform NIH/3T3 cells. Deletion of the 0.8-kilobase SacI fragment abolished the biological activity of c-Ha-ras1, but its replacement by the HSV-1 enhancer fully restored it. These results confirm the enhancer properties of the HSV-1 immediate-early intergenic region and suggest that c-Ha-ras1 sequences contained within the 0.8-kilobase SacI fragment plays a role in the transcriptional activation of the oncogene.

Bibliography

Puga, A., Gomez-Marquez, J., Brayton, P. R., Cantin, E. M., Long, L. K., Barbacid, M., & Notkins, A. L. (1985). The immediate-early enhancer element of herpes simplex virus type 1 can replace a regulatory region of the c-Ha-ras1 oncogene required for transformation. Journal of Virology, 54(3), 879â881.

Authors 7

A Puga (first)
J Gomez-Marquez (additional)
P R Brayton (additional)
E M Cantin (additional)
L K Long (additional)
M Barbacid (additional)
A L Notkins (additional)

References 0 Referenced 12

None

Dates

Type	When
Created	5 years, 7 months ago (Jan. 6, 2020, 9:23 a.m.)
Deposited	3 years, 5 months ago (March 4, 2022, 6:01 p.m.)
Indexed	1 year, 11 months ago (Sept. 16, 2023, 4:02 p.m.)
Issued	40 years, 3 months ago (June 1, 1985)
Published	40 years, 3 months ago (June 1, 1985)
Published Print	40 years, 3 months ago (June 1, 1985)

Funders 0

None

BibTeX

@article{Puga_1985, title={The immediate-early enhancer element of herpes simplex virus type 1 can replace a regulatory region of the c-Ha-ras1 oncogene required for transformation}, volume={54}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.54.3.879-881.1985}, DOI={10.1128/jvi.54.3.879-881.1985}, number={3}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Puga, A and Gomez-Marquez, J and Brayton, P R and Cantin, E M and Long, L K and Barbacid, M and Notkins, A L}, year={1985}, month=jun, pages={879–881} }

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