DOI: 10.1128/jvi.36.1.79-92.1980. Transcripts of the adeno-associated virus genome: mapping of the major RNAs

Transcripts of the adeno-associated virus genome: mapping of the major RNAs

10.1128/jvi.36.1.79-92.1980

Crossref journal-article

American Society for Microbiology

Journal of Virology (235)

Abstract

The four major adeno-associated virus type 2 (AAV2)-specific RNAs were mapped on the linear viral genome by a variety of biochemical techniques, including S1 nuclease and exonuclease VII mapping, RNA gel-transfer hybridization, and analysis of reverse transcriptase extension products. All the major AAV2 RNAs were derived from the minus DNA strand and had 3' termini at position 96. The nucleus-specific 4.3- and 3.6-kilobase (kb) RNAs had 5' termini at positions 6 and 19, respectively. The 5' terminus of the 2.6-kb RNA mapped to position 38.5. The predominant 2.3-kb AAV2 mRNA was spliced and contained a short leader sequence (approximately 50 nucleotides) which mapped to position 38.5, coincident with the 5' terminus of the 2.6-kb RNA. The 5' end of the body of the 2.3-kb RNA mapped to position 46.5. These results are discussed in terms of the involvement of single versus multiple promoters (for transcription) and RNA splicing mechanisms in the generation of the AAV2 RNAs.

Bibliography

Green, M. R., & Roeder, R. G. (1980). Transcripts of the adeno-associated virus genome: mapping of the major RNAs. Journal of Virology, 36(1), 79â92.

Authors 2

M R Green (first)
R G Roeder (additional)

References 0 Referenced 59

None

Dates

Type	When
Created	5 years, 7 months ago (Jan. 5, 2020, 7:54 p.m.)
Deposited	3 years, 6 months ago (March 4, 2022, 5:32 p.m.)
Indexed	11 months, 2 weeks ago (Sept. 16, 2024, 3:12 p.m.)
Issued	44 years, 11 months ago (Oct. 1, 1980)
Published	44 years, 11 months ago (Oct. 1, 1980)
Published Print	44 years, 11 months ago (Oct. 1, 1980)

Funders 0

None

BibTeX

@article{Green_1980, title={Transcripts of the adeno-associated virus genome: mapping of the major RNAs}, volume={36}, ISSN={1098-5514}, url={http://dx.doi.org/10.1128/jvi.36.1.79-92.1980}, DOI={10.1128/jvi.36.1.79-92.1980}, number={1}, journal={Journal of Virology}, publisher={American Society for Microbiology}, author={Green, M R and Roeder, R G}, year={1980}, month=oct, pages={79–92} }

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