DOI: 10.1128/jb.99.3.842-850.1969. Unlinking of Cell Division from Deoxyribonucleic Acid Replication in a Temperature-sensitive Deoxyribonucleic Acid Synthesis Mutant of <i>Escherichia coli</i>

Unlinking of Cell Division from Deoxyribonucleic Acid Replication in a Temperature-sensitive Deoxyribonucleic Acid Synthesis Mutant of Escherichia coli

10.1128/jb.99.3.842-850.1969

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

A new type of temperature-sensitive deoxyribonucleic acid (DNA) synthesis mutant, which can divide without a completion of DNA replication, was isolated from a thymidine-requiring Escherichia coli strain by means of photo-bromouracil selection after nitrosoguanidine mutagenesis. In this mutant, in spite of the fact that DNA synthesis stopped immediately after the temperature shift from 30 to 41 C, cells could continue to divide, though at a reduced rate. This cell division without DNA synthesis at 41 C is further supported by the following results. (i) Cell division took place at high temperature without addition of thymidine but not at all at 30 C. The parent strain of the mutant did not divide at 41 C without thymidine. (ii) Smaller cells isolated from the culture grown at 41 C did not contain DNA. This was shown by chemical analysis of the smaller cells and on electron micrographs. Ability of cells to divide was examined according to sizes of cells. By using the culture at 30 C, cells of various sizes were separated by means of sucrose-density gradient centrifugation. It was found that all cell fractions, including the smallest one, could divide at high temperature. These results suggest that in this mutant the completion of DNA replication is not required for triggering cell division at high temperature. Heat sensitivity of a factor which links cell division with DNA replication appears to be responsible. Some possible mechanisms of the coordination between cell division and DNA replication are discussed.

Bibliography

Inouye, M. (1969). Unlinking of Cell Division from Deoxyribonucleic Acid Replication in a Temperature-sensitive Deoxyribonucleic Acid Synthesis Mutant of Escherichia coli. Journal of Bacteriology, 99(3), 842â850.

Authors 1

Masayori Inouye (first)

References 23 Referenced 68

Adams M. H. 1959. Bacteriophages. Interscience Publishers Inc. New York.
Adler , H. I. , W. D. Fisher , A. Cohen , and A. A. Hardigree . 1967 . Miniature Escherichia coil cells deficient in DNA . Proc. Nat. Acad. Sci. U.S.A. 57 : 321 - 326 . (10.1073/pnas.57.2.321) / Proc. Nat. Acad. Sci. U.S.A. / Miniature Escherichia coil cells deficient in DNA by Adler H. I. (1967)
Bonhoeffer , F. 1966 . DNA transfer and DNA synthesis during bacterial conjugation . Z. Vererbungsl. 98 : 141 - 149 . / Z. Vererbungsl. / DNA transfer and DNA synthesis during bacterial conjugation by Bonhoeffer F. (1966)
Bonhoeffer , F. , and H. Schaller . 1965 . A method for selective enrichment of mutants based on the high UV sensitivity of DNA containing 5-bromouracil . Biochim. Biophys. Res. Commun. 20 : 93 - 97 . / Biochim. Biophys. Res. Commun. / A method for selective enrichment of mutants based on the high UV sensitivity of DNA containing 5-bromouracil by Bonhoeffer F. (1965)
Boyle , J. V. , T. M. Cook , and W. A. Goss . 1969 . Mechanism of action of nalidixic acid on Escherichia coli. VI. Cell-free studies . J. Bacteriol. 97 : 230 - 236 . (10.1128/JB.97.1.230-236.1969) / J. Bacteriol. / Mechanism of action of nalidixic acid on Escherichia coli. VI. Cell-free studies by Boyle J. V. (1969)
Clark , D. J. 1968 . Regulation of deoxyribonucleic acid replication and cell division in Escherichia coli B/r . J. Bacteriol. 96 : 1214 - 1224 . (10.1128/jb.96.4.1214-1224.1968) / J. Bacteriol. / Regulation of deoxyribonucleic acid replication and cell division in Escherichia coli B/r by Clark D. J. (1968)
Cooper , S. , and C. E. Helmstetter . 1968 . Chromosome replication and the division cycle of Escherichia coli B/r . J. Mol. Biol. 31 : 519 - 540 . (10.1016/0022-2836(68)90425-7) / J. Mol. Biol. / Chromosome replication and the division cycle of Escherichia coli B/r by Cooper S. (1968)
Dubnau , E. , and W. K. Mass . 1968 . Inhibition of replication of an F'lac episome in Hfr cells of Escherichia coli . J. Bacteriol. 95 : 531 - 539 . (10.1128/jb.95.2.531-539.1968) / J. Bacteriol. / Inhibition of replication of an F'lac episome in Hfr cells of Escherichia coli by Dubnau E. (1968)
Fangman , W. L. , and A. Novick . 1968 . Characterization of two bacterial mutants with temperature-sensitive synthesis of DNA . Genetics 60 : 1 - 17 . (10.1093/genetics/60.1.1) / Genetics / Characterization of two bacterial mutants with temperature-sensitive synthesis of DNA by Fangman W. L. (1968)
Giles , K. W. , and A. Myers . 1965 . An improved diphenylamine method for the estimation of deoxyribonucleic acid . Nature 206 : 93 . (10.1038/206093a0) / Nature / An improved diphenylamine method for the estimation of deoxyribonucleic acid by Giles K. W. (1965)
Helmstetter , C. E. , and 0. Pierucci . 1968 . Cell division during inhibition of deoxyribonucleic acid synthesis in Escherichia coli . J. Bacteriol. 95 : 1627 - 1633 . (10.1128/jb.95.5.1627-1633.1968) / J. Bacteriol. / Cell division during inhibition of deoxyribonucleic acid synthesis in Escherichia coli by Helmstetter C. E. (1968)
Hirota , Y. , F. Jacob , A. Ryter , G. Buttin , and T. Nakai . 1968 . On the process of cellular division in Escherichia coli. I. Asymmetrical cell division and production of deoxyribonucleic acid-less bacteria . J. Mol. Biol. 35 : 175 - 192 . / J. Mol. Biol. / On the process of cellular division in Escherichia coli. I. Asymmetrical cell division and production of deoxyribonucleic acid-less bacteria by Hirota Y. (1968)
Hirota , Y. , A. Ryter , and F. Jacob . 1968 . Thermosensitive mutants of E. coli affected in the processes of DNA synthesis and cellular division . Cold Spring Harbor Symp. Quant. Biol. 33 : 677 - 693 . (10.1101/SQB.1968.033.01.077) / Cold Spring Harbor Symp. Quant. Biol. / Thermosensitive mutants of E. coli affected in the processes of DNA synthesis and cellular division by Hirota Y. (1968)
Kaiser , A. D. , and D. S. Hogness . 1960 . The transformation of Escherichia coli with deoxyribonucleic acid isolated from bacteriophage Xdg . J. Mol. Biol. 2 : 392 - 415 . (10.1016/S0022-2836(60)80050-2) / J. Mol. Biol. / The transformation of Escherichia coli with deoxyribonucleic acid isolated from bacteriophage Xdg by Kaiser A. D. (1960)
Kohiyama , M. , D. Cousin , A. Ryter , and F. Jacob . 1966 . Mutants thermosensibles d'Escherichia coli K12. I. Isolement et characterisation rapide . Ann. Inst. Pasteur 110 : 456 - 486 . / Ann. Inst. Pasteur / Mutants thermosensibles d'Escherichia coli K12. I. Isolement et characterisation rapide by Kohiyama M. (1966)
Lehman I. R. 1963. DNA synthesis (bacterial) p. 34-39. In S. P. Colowick and N. 0. Kaplan (ed.) Methods in enzymology vol. 6. Academic Press Inc. New York. (10.1016/0076-6879(63)06145-0)
Lowry , 0. H. , N. J. Rosebrough , A. L. Farr , and R. J. Randall . 1951 . Protein measurement with the Folin phenol reagent . J. Biol. Chem. 193 : 265 - 275 . (10.1016/S0021-9258(19)52451-6) / J. Biol. Chem. / Protein measurement with the Folin phenol reagent by Lowry 0. (1951)
Mendelson , N. H. , and J. D. Gross . 1967 . Characterization of a temperature-sensitive mutant of Bacillus subtilis defective in deoxyribonucleic acid replication . J. Bacteriol. 94 : 1603 - 1608 . (10.1128/JB.94.5.1603-1608.1967) / J. Bacteriol. / Characterization of a temperature-sensitive mutant of Bacillus subtilis defective in deoxyribonucleic acid replication by Mendelson N. H. (1967)
Mitchison , J. M. , and W. S. Vincent . 1965 . Preparation of synchronous cultures by sedimentation . Nature 205 : 987 - 989 . (10.1038/205987a0) / Nature / Preparation of synchronous cultures by sedimentation by Mitchison J. M. (1965)
Peters V. D. and R. Wigand. 1953. Enzymatisch-electronenoptische analyse der nucleinsaureverteilung dargestellt an Escherichia coli als modell. Z. Naturforschung 8b:180-192. (10.1515/znb-1953-0404)
Prestidge , L. S. , and A. B. Pardee . 1965 . A second permease for methyl-thio-O-D-galactoside in Escherichia coli . Biochim. Biophys. Acta 100 : 591 - 593 . (10.1016/0304-4165(65)90029-2) / Biochim. Biophys. Acta / A second permease for methyl-thio-O-D-galactoside in Escherichia coli by Prestidge L. S. (1965)
Smith , H. S. , and L. I. Pizer . 1968 . Abortive infection of Escherichia coli strain W by T2 bacteriophage . J. Mol. Biol. 37 : 131 - 149 . (10.1016/0022-2836(68)90078-8) / J. Mol. Biol. / Abortive infection of Escherichia coli strain W by T2 bacteriophage by Smith H. S. (1968)
Witkin , E. M. 1967 . The radiation sensitivity of Escherichia coli B: a hypothesis relating filament formation and prophage induction . Proc. Nat. Acad. Sci. U.S.A. 57 : 1275 - 1279 . (10.1073/pnas.57.5.1275) / Proc. Nat. Acad. Sci. U.S.A. / The radiation sensitivity of Escherichia coli B: a hypothesis relating filament formation and prophage induction by Witkin E. M. (1967)

Dates

Type	When
Created	5 years, 7 months ago (Jan. 4, 2020, 9:20 p.m.)
Deposited	4 years, 3 months ago (May 31, 2021, 9:59 a.m.)
Indexed	1 year, 9 months ago (Nov. 2, 2023, 9:09 p.m.)
Issued	56 years ago (Sept. 1, 1969)
Published	56 years ago (Sept. 1, 1969)
Published Print	56 years ago (Sept. 1, 1969)

Funders 0

None

BibTeX

@article{Inouye_1969, title={Unlinking of Cell Division from Deoxyribonucleic Acid Replication in a Temperature-sensitive Deoxyribonucleic Acid Synthesis Mutant of Escherichia coli}, volume={99}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.99.3.842-850.1969}, DOI={10.1128/jb.99.3.842-850.1969}, number={3}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Inouye, Masayori}, year={1969}, month=sep, pages={842–850} }

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