DOI: 10.1128/jb.96.3.665-671.1968. Electron Transport System of the Protoheme-requiring Anaerobe <i>Bacteroides melaninogenicus</i>

Electron Transport System of the Protoheme-requiring Anaerobe Bacteroides melaninogenicus

10.1128/jb.96.3.665-671.1968

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

Protoheme is essential for the growth of some strains of Bacteroides melaninogenicus . At low concentrations in the growth medium, protoheme determines the doubling time, total cell yield, and amount of cytochrome per bacterium. At high protoheme concentrations, the doubling time, total cell yield, and amount of enzymatically reducible cytochrome appear to remain nearly constant, and protoheme is accumulated by the cell. The accumulated protoheme can support the growth of the bacterium for at least eight generations in a protoheme-free medium. When growth and cytochrome content are proportional during growth at low protoheme concentrations, the bacteria incorporate 10 to 20% of the total available protoheme into a membrane-bound respiratory system. This respiratory system includes cytochrome c , a carbon monoxide-binding pigment, and possibly flavoproteins. The pigments can be reversibly reduced by reduced nicotinamide adenine dinucleotide or endogenous metabolism and can be oxidized anaerobically by fumarate or by shaking in air. Electron transport is inhibited by 2- n -nonyl-4-hydroxy-quinoline- N -oxide.

Bibliography

Rizza, V., Sinclair, P. R., White, D. C., & Cuorant, P. R. (1968). Electron Transport System of the Protoheme-requiring Anaerobe Bacteroides melaninogenicus. Journal of Bacteriology, 96(3), 665â671.

Authors 4

Victor Rizza (first)
Peter R. Sinclair (additional)
David C. White (additional)
Paul R. Cuorant (additional)

References 18 Referenced 43

Estabrook R. W. 1961. Spectrophotometric studies of cytochromes cooled in liquid nitrogen p. 436-459. In J. E. Falk R. Lemberg and R. K. Morton (ed.) Haematin enzymes. Pergamon Press New York.
Falk J. E. 1964. Porphyrins and metalloporphyrins. Elsevier Publishing Co. New York.
Frerman , F. E. , and D. C. White . 1967 . Membrane lipid changes during formation of a functional electron transport system in Staphylococcus aureus . J. Bacteriol. 94 : 1868 - 1874 . (10.1128/JB.94.6.1868-1874.1967) / J. Bacteriol. / Membrane lipid changes during formation of a functional electron transport system in Staphylococcus aureus by Frerman F. E. (1967)
Gibbons , R. J. , and L. P. Engle . 1964 . Vitamin K compounds in bacteria that are obligate aerobes . Science 146 : 1307 - 1309 . (10.1126/science.146.3649.1307) / Science / Vitamin K compounds in bacteria that are obligate aerobes by Gibbons R. J. (1964)
Gibbons , R. J. , and J. B. MacDonald . 1960 . Hemin and vitamin K compounds as required factors for the cultivation of certain strains of Bacteroides melaninogenicus . J. Bacteriol. 80 : 164 - 170 . (10.1128/JB.80.2.164-170.1960) / J. Bacteriol. / Hemin and vitamin K compounds as required factors for the cultivation of certain strains of Bacteroides melaninogenicus by Gibbons R. J. (1960)
Gibbons , R. J. , and J. B. MacDonald . 1961 . Degradation of collagenous substrates by Bacteroides melaninogenicus . J. Bacteriol. 81 : 614 - 621 . (10.1128/JB.81.4.614-621.1961) / J. Bacteriol. / Degradation of collagenous substrates by Bacteroides melaninogenicus by Gibbons R. J. (1961)
Henderson R. W. and D. D. Nankiville. 1966. Electrophoretic and other studies on haem pig-671
Lev , M. 1958 . Apparent requirement for vitamin K of rumen strains of Fusiformis nigrescens . Nature 181 : 203 - 204 . (10.1038/181203a0) / Nature / Apparent requirement for vitamin K of rumen strains of Fusiformis nigrescens by Lev M. (1958)
Lowry , 0. H. , N. J. Rosenbough , A. L. Farr , and R. J. Randall . 1951 . Protein measurement with the Folin phenol reagent . J. Biol. Chem. 193 : 265 - 275 . (10.1016/S0021-9258(19)52451-6) / J. Biol. Chem. / Protein measurement with the Folin phenol reagent by Lowry 0. (1951)
Sawyer , S. J. , J. B. MacDonald , and R. J. Gibbons . 1962 . Biochemical characteristics of Bacteroides melaninogenicus . Arch. Oral. Biol. 7 : 685 - 691 . (10.1016/0003-9969(62)90117-6) / Arch. Oral. Biol. / Biochemical characteristics of Bacteroides melaninogenicus by Sawyer S. J. (1962)
Schwabacher , H. , D. R. Lucas , and C. Rimington . 1947 . Bacterium melaninogenicum-a misnomer . J. Gen. Microbiol. 1 : 109 - 120 . (10.1099/00221287-1-2-109) / J. Gen. Microbiol. / Bacterium melaninogenicum-a misnomer by Schwabacher H. (1947)
Smith , L. 1954 . Bacterial cytochromes . Bacteriol. Rev. 18 : 106 - 130 . (10.1128/BR.18.2.106-130.1954) / Bacteriol. Rev. / Bacterial cytochromes by Smith L. (1954)
White , D. C. 1963 . Respiratory systems in the hemin-requiring Haemophilus species . J. Bacteriol. 85 : 84 - 96 . (10.1128/jb.85.1.84-96.1963) / J. Bacteriol. / Respiratory systems in the hemin-requiring Haemophilus species by White D. C. (1963)
White , D. C. , 1965 . Synthesis of 2-demethyl vitamin K2 and the cytochrome system of Haemophilus . J. Bacteriol. 89 : 299 - 305 . (10.1128/JB.89.2.299-305.1965) / J. Bacteriol. / Synthesis of 2-demethyl vitamin K2 and the cytochrome system of Haemophilus by White D. C. (1965)
White , D. C. 1966 . The obligatory involvement of the electron transport system in the catabolic metabolism of Hemophilus parainfluenza. Antonie van Leeuwenhoek . J. Microbiol. Serol. 32 : 139 - 158 . / J. Microbiol. Serol. / The obligatory involvement of the electron transport system in the catabolic metabolism of Hemophilus parainfluenza. Antonie van Leeuwenhoek by White D. C. (1966)
White , D. C. 1967 . Effect of glucose on the formation of the membrane-bound electron transport system in Haemophilus parainfluenza . J. Bacteriol. 93 : 567 - 573 . (10.1128/JB.93.2.567-573.1967) / J. Bacteriol. / Effect of glucose on the formation of the membrane-bound electron transport system in Haemophilus parainfluenza by White D. C. (1967)
White , D. C. , M. P. Bryant , and D. R. Caldwell . 1962 . Cytochrome-linked fermentation in Bacteroides ruminicola . J. Bacteriol. 84 : 822 - 828 . (10.1128/JB.84.4.822-828.1962) / J. Bacteriol. / Cytochrome-linked fermentation in Bacteroides ruminicola by White D. C. (1962)
Yamanaka , R. , H. De Klerk , and M. D. Kamen . 1967 . Highly purified cytochrome c derived from the diatom Navicula pelliculosa . Biochim. Biophys. Acta 143 : 416 - 424 . (10.1016/0005-2728(67)90095-3) / Biochim. Biophys. Acta / Highly purified cytochrome c derived from the diatom Navicula pelliculosa by Yamanaka R. (1967)

Dates

Type	When
Created	5 years, 8 months ago (Jan. 4, 2020, 9:09 p.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 12:47 p.m.)
Indexed	1 year, 9 months ago (Nov. 23, 2023, 6:16 a.m.)
Issued	57 years ago (Sept. 1, 1968)
Published	57 years ago (Sept. 1, 1968)
Published Print	57 years ago (Sept. 1, 1968)

Funders 0

None

BibTeX

@article{Rizza_1968, title={Electron Transport System of the Protoheme-requiring Anaerobe Bacteroides melaninogenicus}, volume={96}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.96.3.665-671.1968}, DOI={10.1128/jb.96.3.665-671.1968}, number={3}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Rizza, Victor and Sinclair, Peter R. and White, David C. and Cuorant, Paul R.}, year={1968}, month=sep, pages={665–671} }

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