Morphology and Chemistry of Cell Walls ofMicrococcus radiodurans
10.1128/jb.95.2.641-657.1968
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

Walls of the pigmented strain ofMicrococcus radioduransshowed several layers in the electron microscope. These layers include an outermost network structure removed by trypsin, a fragile soft layer containing hexagonally packed subunits, and a rigid layer penetrated by numerous holes. The two inner layers were separated by a process of autolysis, trypsin treatment, and gradient centrifugation. The hexagonally packed layer was less dense, pink in color, and it contained carotenoids, lipid, protein, and polysaccharide. The lipid consisted of odd-numbered as well as even-numbered fatty acids, and the polysaccharide contained rhamnose and mannose, but it did not contain heptose. The “holey” layer was white and was composed of a mucopeptide containing glucosamine, muramic acid, and four main amino acids (glutamic acid, alanine, glycine, andl-ornithine, in the ratios of 1:1.7:1.8:1.2, respectively). This layer also contained phosphorus, glucose, and a trace ofmeso- andll-diaminopimelic acid. A white mutant, W1, ofM. radioduranshad no pigment or lipid in its walls, but it contained small amounts of the “hexagonal” layer. The holey layer, constituting the bulk of the wall, was similar in morphology and composition to that layer in the pigmented strain. Lysozyme did not remove the lipoprotein-polysaccharide component from the walls of the pigmented strains, and the hexagonally packed structure was not visibly affected, except for change in a minor structure. Most of the mucopeptide layer was solubilized by lysozyme, but a structureless bag-shaped residue was left. This residue contained phosphorus, carbohydrate, and limited amino acids, but it did not contain muramic acid, glucosamine, or ornithine. Aqueous phenol removed a lipoprotein component from strain R1, which contained limited fatty acids. It also removedmeso- andll-diaminopimelic acid.

Bibliography

Work, E., & Griffiths, H. (1968). Morphology and Chemistry of Cell Walls ofMicrococcus radiodurans. Journal of Bacteriology, 95(2), 641–657.

Authors 2
  1. Elizabeth Work (first)
  2. Hilary Griffiths (additional)
References 40 Referenced 63
  1. ALLEN , R. J. L. 1940 . The estimation of phosphorus . Biochem. J. 34 : 858 - 865 . (10.1042/bj0340858) / Biochem. J. / The estimation of phosphorus by ALLEN R. J. (1940)
  2. ALLSOP , J. , AND E. WORK. 1963 . Cell walls of Propionibacterium species: fractionation and composition . Biochem. J. 87 : 512 - 519 . (10.1042/bj0870512) / Biochem. J. / Cell walls of Propionibacterium species: fractionation and composition by ALLSOP J. (1963)
  3. ANDERSON , A. W. , H. C. NORDAN , R. F. CAIN , G. PARRISH , A ND D . E. DUGGAN. 1956 . Studies on a radiation-resistant micrococcus. I. Isolation, morphology, cultural characteristics and resistance of -y-radiation . Food. Technol. 10 : 575 - 577 . / Food. Technol. / Studies on a radiation-resistant micrococcus. I. Isolation, morphology, cultural characteristics and resistance of -y-radiation by ANDERSON A. W. (1956)
  4. BAIRD-PARKER , A. C. 1965 . The classification of Staphylococci and Micrococci from worldwide sources . J. Gen. Microbiol. 38 : 363 - 387 . (10.1099/00221287-38-3-363) / J. Gen. Microbiol. / The classification of Staphylococci and Micrococci from worldwide sources by BAIRD-PARKER A. C. (1965)
  5. BIRDSELL , D. C. , AND E. H. COTA-ROBLES . 1967 . Production and ultrastructure of Iysozyme and ethylenediaminetetraacetate-lysozyme spheroplasts of Escherichia coli . J. Bacteriol. 93 : 427 - 437 . (10.1128/JB.93.1.427-437.1967) / J. Bacteriol. / Production and ultrastructure of Iysozyme and ethylenediaminetetraacetate-lysozyme spheroplasts of Escherichia coli by BIRDSELL D. C. (1967)
  6. BLEIWEIS , A. S. , AND R. M. KRAUSE. 1965 . The cell walls of Group D streptococci. I. The immunochemistry of the type 1 carbohydrate . J. Exptl. Med. 122 : 237 - 249 . (10.1084/jem.122.2.237) / J. Exptl. Med. / The cell walls of Group D streptococci. I. The immunochemistry of the type 1 carbohydrate by BLEIWEIS A. S. (1965)
  7. BOLING , M. E. , AND J. K. SETLOW. 1966 . The resistance of Micrococcus radiodurans to ultraviolet radiation. III. A repair mechanism . Biochim. Biophys. Acta 123 : 26 - 33 . (10.1016/0005-2787(66)90155-9) / Biochim. Biophys. Acta / The resistance of Micrococcus radiodurans to ultraviolet radiation. III. A repair mechanism by BOLING M. E. (1966)
  8. CHAPMAN , J. A. , R. G. E. MURRAY , A ND M . R. J. SALTON. 1963 . The surface anatomy of Lampropedia hyalina . Proc. Roy. Soc. (London) Ser. B 158 : 498 - 513 . / Proc. Roy. Soc. (London) Ser. B / The surface anatomy of Lampropedia hyalina by CHAPMAN J. A. (1963)
  9. CHEN , P. S. , T. Y. TORIBARA , A ND H . WARNER. 1956 . Microdetermination of phosphorus . Anal. Chem. 28 : 1756 - 1758 . (10.1021/ac60119a033) / Anal. Chem. / Microdetermination of phosphorus by CHEN P. S. (1956)
  10. DISCHE , Z. 1962 . Color reactions of heptoses and octoses . Methods in Carbohydrate Chemistry 1 : 494 - 496 . / Methods in Carbohydrate Chemistry / Color reactions of heptoses and octoses by DISCHE Z. (1962)
  11. DUBOILS , M., K. A. GILLES , J. K. HAMILTON , P. A. REBERS , A ND F . SMITH. 1956 . Colorimetric method for determination of sugars and related substances . Anal. Chem. 28 : 350 - 356 . (10.1021/ac60111a017) / Anal. Chem. / Colorimetric method for determination of sugars and related substances by DUBOILS M., K. (1956)
  12. Duc-NGUYEN H. AND L. L. WEED. 1964. DJ. BACTER10L.
  13. DUGGAN , D. E. , A. W. ANDERSON , P. R. ELLIKER , A ND R . F. CAIN. 1959 . Ultraviolet exposure studies on a gamma radiation resistant micrococcus isolated from food . Food. Res. 24 : 376 - 382 . (10.1111/j.1365-2621.1959.tb17287.x) / Food. Res. / Ultraviolet exposure studies on a gamma radiation resistant micrococcus isolated from food by DUGGAN D. E. (1959)
  14. GLAUERT , A. M. 1962 . The fine structure of bacteria . Brit. Med. Bull. 18 : 245 - 250 . (10.1093/oxfordjournals.bmb.a069988) / Brit. Med. Bull. / The fine structure of bacteria by GLAUERT A. M. (1962)
  15. GLAUERT , A. M. 1966 . Moire patterns in electron micrographs of a bacterial membrane . J. Cell Sci. 1 : 425 - 428 . (10.1242/jcs.1.4.425) / J. Cell Sci. / Moire patterns in electron micrographs of a bacterial membrane by GLAUERT A. M. (1966)
  16. HUGGET , A. ST . G. , AND D. A. NIXON. 1957 . The use of glucose oxidase, peroxidase and o-dianisidine in determination of blood and urine glucose . Lancet 2 : 368 - 370 . (10.1016/S0140-6736(57)92595-3) / Lancet / The use of glucose oxidase, peroxidase and o-dianisidine in determination of blood and urine glucose by HUGGET A. (1957)
  17. KELLENBERGER , E. , AND E. BOY DE LA TOUR . 1965 . Studies on the morphopoiesis of the head of phage T-even. II. Observations on the fine structure of polyheads . J. Ultrastruct. Res. 13 : 343 - 358 . (10.1016/S0022-5320(65)80081-8) / J. Ultrastruct. Res. / Studies on the morphopoiesis of the head of phage T-even. II. Observations on the fine structure of polyheads by KELLENBERGER E. (1965)
  18. KELLENBERGER , E., A. RYTER , A ND J . SECHAUD. 1958 . Electron microscope study of DNA-containing plasms. II. Vegetative and mature phage DNA compared with normal bacterial nucleoids in different physiological states . J. Biophys. Biochem. Cytol. 4 : 671 - 678 . / J. Biophys. Biochem. Cytol. / Electron microscope study of DNA-containing plasms. II. Vegetative and mature phage DNA compared with normal bacterial nucleoids in different physiological states by KELLENBERGER E., A. (1958)
  19. KNI vE-r-r, V. A., J. CULLEN , A ND M . JACKSON. 1965 . Odd-numbered fatty acids in Micrococcus radiodurans . Biochem. J. 96 : 2 - 3 C. (10.1042/bj0960002C) / Biochem. J. / Odd-numbered fatty acids in Micrococcus radiodurans by KNI (1965)
  20. MARTIN H. H. AND H. FRANK. 1962. Quantitative Bausteinanalyse der Stutzmembran in der Zellwand von Escherichia coli B. Z. Naturforsch. 17b:190-196. (10.1515/znb-1962-0312)
  21. MILLER , I., R. PLAPP , AND 0. KAN DLER . 1966 . The amino acid sequence of the serine containing murein of Butyribacterium rettgeri . Biochem. Biophys. Res. Commun. 25 : 415 - 420 . (10.1016/0006-291X(66)90221-X) / Biochem. Biophys. Res. Commun. / The amino acid sequence of the serine containing murein of Butyribacterium rettgeri by MILLER I., R. (1966)
  22. MOSELEY , B. E. B. 1963 . The variation in X-ray resistance of Micrococcus radiodurans and some of its less-pigmented mutants . Intern. J. Radiation Biol. 6 : 489 . / Intern. J. Radiation Biol. / The variation in X-ray resistance of Micrococcus radiodurans and some of its less-pigmented mutants by MOSELEY B. E. (1963)
  23. MOSELEY , B. E. B., AND H. LASER. 1965 . Repair of X-ray damage in Micrococcus radioduranis . Proc. Roy. Soc. (London) Ser. B 162 : 210 - 222 . / Proc. Roy. Soc. (London) Ser. B / Repair of X-ray damage in Micrococcus radioduranis by MOSELEY B. E. (1965)
  24. MOSELEY , B. E. B., AND A. H. SCHEIN. 1964 . Radiation resistance and deoxyribonucleic acid base composition of Micrococcus radiodurans . Nature 203 : 1298 - 1299 . (10.1038/2031298a0) / Nature / Radiation resistance and deoxyribonucleic acid base composition of Micrococcus radiodurans by MOSELEY B. E. (1964)
  25. MURRAY , R. G. E. 1962 . Fine structure and taxonomy of bacteria . Symp. Soc. Gen. Microbiol. 12 : 119 - 144 . / Symp. Soc. Gen. Microbiol. / Fine structure and taxonomy of bacteria by MURRAY R. G. (1962)
  26. MURRAY , R. G. E. 1963 . On the cell wall structure of Spirillum serpens . Can. J. Microbiol. 9 : 381 - 392 . (10.1139/m63-046) / Can. J. Microbiol. / On the cell wall structure of Spirillum serpens by MURRAY R. G. (1963)
  27. PERKINS , H. R. , AND C. S. CUMMINS. 1964 . Ornithine and 2,4-diaminobutyric acid as components of the cell walls of plant pathogenic Corynebacteria . Nature 201 : 1105 - 1107 . (10.1038/2011105a0) / Nature / Ornithine and 2,4-diaminobutyric acid as components of the cell walls of plant pathogenic Corynebacteria by PERKINS H. R. (1964)
  28. RIBI , E. , AND B. H. HOYER. 1960 . Purification of Q-fever rickettsiae by density-gradient sedimentation . J. Immunol. 85 : 314 - 318 . (10.4049/jimmunol.85.3.314) / J. Immunol. / Purification of Q-fever rickettsiae by density-gradient sedimentation by RIBI E. (1960)
  29. RHULAND L. E. E. WORK R. F. DENMAN AND
  30. VOL . 95 , 1968 CELL WALLS OF A W. RADIODURANS D. S. HOARE. 1955. The behaviour of isomers of a, e-diaminopimelic acid on paper chromatograms . J. Am. Chem. Soc. 77 : 4844 - 4846 . / J. Am. Chem. Soc. / CELL WALLS OF A W. RADIODURANS D. S. HOARE. 1955. The behaviour of isomers of a, e-diaminopimelic acid on paper chromatograms by VOL (1968)
  31. ROTH , H., S. SEGAL , A ND D . BERTOLI. 1965 . The quantitative determination of galactose-an enzymic method using galactose oxidase, with applications to blood and other biological fluids . Anal. Biochem. 10 : 32 - 52 . (10.1016/0003-2697(65)90238-1) / Anal. Biochem. / The quantitative determination of galactose-an enzymic method using galactose oxidase, with applications to blood and other biological fluids by ROTH H., S. (1965)
  32. SALTON , M. R. J., AND A. F. M. EHTISHAM-UDDIN . 1965 . The localisation of cytochromes and carotenoids in isolated bacterial membranes and envelopes . Austr. J. Exptl. Biol. Med. Sci. 43 : 255 - 264 . (10.1038/icb.1965.24) / Austr. J. Exptl. Biol. Med. Sci. / The localisation of cytochromes and carotenoids in isolated bacterial membranes and envelopes by SALTON M. R. (1965)
  33. SHOCKMAN , G. D. , J. J. KOLB , A ND G . TOENNIES. 1957 . A high speed shaker for the disruption of cells at low temperatures . Biochim. Biophys. Acta 24 : 203 - 204 . (10.1016/0006-3002(57)90168-3) / Biochim. Biophys. Acta / A high speed shaker for the disruption of cells at low temperatures by SHOCKMAN G. D. (1957)
  34. SHOCKMAN , G. D. , J. S. THOMPSON , A ND M . J. CONOVER. 1967 . The autolytic enzyme system of Streptococcus faecalis. II. Partial characterisation of the autolysin and its substrate . Biochemistry 6 : 1054 - 1065 . 657 (10.1021/bi00856a014) / Biochemistry / The autolytic enzyme system of Streptococcus faecalis. II. Partial characterisation of the autolysin and its substrate by SHOCKMAN G. D. (1967)
  35. SPACKMAN , D. H. , W. H. STEIN , A ND S . MOORE. 1958 . Automatic recording apparatus for use in the chromatography of amino acids . Anal. Chem. 7 : 1190 - 1206 . (10.1021/ac60139a006) / Anal. Chem. / Automatic recording apparatus for use in the chromatography of amino acids by SPACKMAN D. H. (1958)
  36. THORNLEY , M. J. , R. W. HORNE , A ND A . M. GLAUERT. 1965 . The fine structure of Micrococcus radiodurans . Arch. Mikrobiol. 51 : 267 - 289 . (10.1007/BF00408143) / Arch. Mikrobiol. / The fine structure of Micrococcus radiodurans by THORNLEY M. J. (1965)
  37. TREVELYAN , W. E. , D. D. PROCTER , A ND J . S. HARRISON. 1950 . Detection of sugars on paper chromatograms . Nature 166 : 444 - 445 . (10.1038/166444b0) / Nature / Detection of sugars on paper chromatograms by TREVELYAN W. E. (1950)
  38. VEERKAMP , J. H. , R. LAMBERT , A ND Y . SAITO. 1965 . The composition of the cell wall of Lactobacillus bifidus var pennsylvanicus . Arch. Biochem. Biophys. 112 : 120 - 125 . (10.1016/0003-9861(65)90019-6) / Arch. Biochem. Biophys. / The composition of the cell wall of Lactobacillus bifidus var pennsylvanicus by VEERKAMP J. H. (1965)
  39. WORK , E. 1964 . Amino acids of walls of Micrococcus radiodurans . Nature 201 : 1107 - 1109 . (10.1038/2011107a0) / Nature / Amino acids of walls of Micrococcus radiodurans by WORK E. (1964)
  40. YOSHIDA , A., C. -G. HEDEN , B. CEDERGREN , A ND L . EDEBO. 1961 . A method for the preparation of undigested bacterial cell walls . J. Biochem. Microbiol. Technol. Eng. 3 : 151 - 159 . (10.1002/jbmte.390030205) / J. Biochem. Microbiol. Technol. Eng. / A method for the preparation of undigested bacterial cell walls by YOSHIDA A., C. (1961)
Dates
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Created 5 years, 7 months ago (Jan. 4, 2020, 9:05 p.m.)
Deposited 1 year, 11 months ago (Sept. 24, 2023, 2:46 p.m.)
Indexed 1 month, 3 weeks ago (July 2, 2025, 3:31 p.m.)
Issued 57 years, 6 months ago (Feb. 1, 1968)
Published 57 years, 6 months ago (Feb. 1, 1968)
Published Print 57 years, 6 months ago (Feb. 1, 1968)
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@article{Work_1968, title={Morphology and Chemistry of Cell Walls ofMicrococcus radiodurans}, volume={95}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.95.2.641-657.1968}, DOI={10.1128/jb.95.2.641-657.1968}, number={2}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Work, Elizabeth and Griffiths, Hilary}, year={1968}, month=feb, pages={641–657} }