DOI: 10.1128/jb.94.6.1970-1981.1967. Cold-Sensitive Mutation of <i>Pseudomonas putida</i> Affecting Enzyme Synthesis at Low Temperature

Cold-Sensitive Mutation of Pseudomonas putida Affecting Enzyme Synthesis at Low Temperature

10.1128/jb.94.6.1970-1981.1967

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

A cold-sensitive mutant of Pseudomonas putida has been isolated which grows normally at 30 C but is unable to grow on mandelate as a source of carbon at 15 C. The mutation results in the inability of the strain to carry out the reaction catalyzed by cis,cis -muconate lactonizing enzyme at low temperature and must lie in the structural gene for that enzyme, because the mutant enzyme produced at 30 C shows altered thermal stability. The mutant enzyme is not intrinsically cold-labile, nor is it cold-labile at the moment of synthesis. The activity of the mutant enzyme is not inhibited at low temperature. Evidence is presented to establish that this mutation in the structural gene coding for cis,cis -muconate lactonizing enzyme results in the lack of expression of that gene at low temperature.

Bibliography

Condon, S., & Ingraham, J. L. (1967). Cold-Sensitive Mutation of Pseudomonas putida Affecting Enzyme Synthesis at Low Temperature. Journal of Bacteriology, 94(6), 1970â1981.

Authors 2

Séamus Condon (first)
John L. Ingraham (additional)

References 20 Referenced 18

AMES B. N. R. F. GOLDBERGER P. E. HARTMAN R. G. MARTIN AND J. R. ROTH. 1967. The histidine operon p. 272-287. In V. V. Koningsberger and L. Bosch [ed.] Regulation of nucleic acid and protein biosynthesis. B. B. A. Library vol. 10. Elsevier Publishing Co. Amsterdam.
COHEN-BAZIRE , G., W. R. SISTROM , A ND R . Y. STANIER. 1957 . Kinetic studies of pigment synthesis by non-sulfur purple bacteria . J. Cellular Comp. Physiol. 49 : 25 - 68 . (10.1002/jcp.1030490104) / J. Cellular Comp. Physiol. / Kinetic studies of pigment synthesis by non-sulfur purple bacteria by COHEN-BAZIRE G., W. (1957)
CownE, D. B., S. SPIEGELMAN , R. B. ROBERTS , A ND J . D. DUERKSEN. 1961 . Ribosome-bound ,B-galactosidase . Proc. NatI. Acad. Sci. U. S. 47: 114 - 122 . / Proc. NatI. Acad. Sci. U. / Ribosome-bound ,B-galactosidase by SPIEGELMAN R. B. (1961)
DOUDOROFF , M. , AND C. W. SCHUSTER. 1962 . A cold-sensitive D(-) ,B-hydroxybutyric acid dehydrogenase from Rhodospirillum rubrum . J. Biol. Chem. 237 : 603 - 607 . (10.1016/S0021-9258(18)93969-4) / J. Biol. Chem. / A cold-sensitive D(-) ,B-hydroxybutyric acid dehydrogenase from Rhodospirillum rubrum by DOUDOROFF M. (1962)
FOWLER , A. V. , AND I. ZABIN. 1966 . Co-linearity of f-galactosidase with its gene by immunological detection of incomplete polypeptide chains . Science 154 : 1027 - 1029 . (10.1126/science.154.3752.1027) / Science / Co-linearity of f-galactosidase with its gene by immunological detection of incomplete polypeptide chains by FOWLER A. V. (1966)
GOLDSTEIN , A., D. B. GOLDSTEIN , A ND L . I. Low NEY. 1964 . Protein synthesis at 0° in Escherichia coli . J. Mol. Biol. 9 : 213 - 235 . (10.1016/S0022-2836(64)80102-9) / J. Mol. Biol. / Protein synthesis at 0° in Escherichia coli by GOLDSTEIN A., D. (1964)
HEGEMAN , G. D. 1966 . Synthesis of the enzymes of the mandelate pathway by Pseudomonas putida. I. Synthesis of enzymes by the wild type . J. Bacteriol. 91 : 1140 - 1154 . (10.1128/jb.91.3.1140-1154.1966) / J. Bacteriol. / Synthesis of the enzymes of the mandelate pathway by Pseudomonas putida. I. Synthesis of enzymes by the wild type by HEGEMAN G. D. (1966)
INGRAHAM J. L. AND 0. MAAL0E. 1966. Cold sensitive mutants and the minimum temperature of growth of bacteria p. 297-309. In C. L. Prosser [ed.] Molecular mechanisms of temperature adaptation. American Association for the Advancement of Science Washington D.C.
LOWRY , 0. H., N. J. ROSEBROUGH , A. L. FARR , A ND R . J. RANDALL. 1951 . Protein measurement with the Folin phenol reagent . J. Biol. Chem. 193 : 265 - 275 . (10.1016/S0021-9258(19)52451-6) / J. Biol. Chem. / Protein measurement with the Folin phenol reagent by LOWRY H., N. (1951)
MAAL0E O. AND N. 0. KJELDGAARD. 1966. Control of macromolecular synthesis. W. A. Benjamin Inc. New York.
MARR , A. G. , J. L. INGRAHAM , A ND C . L. SQUIRES. 1964 . Effect of the temperature of growth of Escherichia coli on the formation of fl-galactosidase . J. Bacteriol. 87 : 356 - 362 . (10.1128/jb.87.2.356-362.1964) / J. Bacteriol. / Effect of the temperature of growth of Escherichia coli on the formation of fl-galactosidase by MARR A. G. (1964)
NG , H., J. L. INGRAHAM , A ND A . G. MARR. 1962 . Damage and derepression in Escherichia coli resulting from growth at low temperatures . J. Bacteriol. 84 : 331 - 339 . (10.1128/jb.84.2.331-339.1962) / J. Bacteriol. / Damage and derepression in Escherichia coli resulting from growth at low temperatures by NG H., J. (1962)
O'DONOVAN , G. A. , AND J. L. INGRAHAM. 1965 Cold-sensitive mutants of Escherichia coli resulting from increased feedback inhibition . Proc. Natl. Acad. Sci. U. S. 54: 451 - 457 . (10.1073/pnas.54.2.451) / Proc. Natl. Acad. Sci. U. / Cold-sensitive mutants of Escherichia coli resulting from increased feedback inhibition by O'DONOVAN G. A. (1965)
O'D oNov AN, G . A., C. L. KEARNEY , A ND J . L. INGRAHAM. 1965 . Mutants of Escherichia coli with high minimal temperatures of growth . J. Bacteriol. 90 : 611 - 616 . (10.1128/jb.90.3.611-616.1965) / J. Bacteriol. / Mutants of Escherichia coli with high minimal temperatures of growth by O'D AN, G (1965)
ORNSTON , L. N. 1966 . The conversion of catechol and protocatechuate to ,B-ketoadipate by Pseudomonas putida. III. Enzymes of the catechol pathway . J. Biol. Chem. 241 : 3795 - 3799 . (10.1016/S0021-9258(18)99841-8) / J. Biol. Chem. / The conversion of catechol and protocatechuate to ,B-ketoadipate by Pseudomonas putida. III. Enzymes of the catechol pathway by ORNSTON L. N. (1966)
ORNSTON , L. N. 1966 . The conversion of catechol and protocatechuate to 3-ketoadipate by Pseudomonas putida . IV. Regulation. J. Biol. Chem. 241 : 3800 - 3810 . / IV. Regulation. J. Biol. Chem. / The conversion of catechol and protocatechuate to 3-ketoadipate by Pseudomonas putida by ORNSTON L. N. (1966)
ORNSTON , L. N. , AND R. Y. STANIER. 1966 . The conversion of catechol and protocatechuate to B-ketoadipate by Pseudomonas putida . I. Biochemistry. J. Biol. Chem. 241 : 3776 - 3786 . (10.1016/S0021-9258(18)99839-X) / I. Biochemistry. J. Biol. Chem. / The conversion of catechol and protocatechuate to B-ketoadipate by Pseudomonas putida by ORNSTON L. N. (1966)
PULLMAN , M. E. , H. S. PENEFSKY , A. DATTA , A ND E . RACKER. 1960 . Partial resolution of the enzymes catalyzing oxidative phosphorylation. I. Purification and properties of soluble, dinitrophenol-stimulated adenosine triphosphatase . J. Biol. Chem. 235 : 3322 - 3329 . (10.1016/S0021-9258(20)81361-1) / J. Biol. Chem. / Partial resolution of the enzymes catalyzing oxidative phosphorylation. I. Purification and properties of soluble, dinitrophenol-stimulated adenosine triphosphatase by PULLMAN M. E. (1960)
SHUKUYA , R. , AND G. W. SCHWERT. 1960 . Glutamic acid decarboxylase. III. The inactivation of the enzyme at low temperatures . J. Biol. Chem. 235 : 1658 - 1661 . / J. Biol. Chem. / Glutamic acid decarboxylase. III. The inactivation of the enzyme at low temperatures by SHUKUYA R. (1960)
STANIER , R. Y. , N. J. PALLERONI , A ND M . Dou- DOROFF. 1966 . The aerobic pseudomonads: a taxonomic study . J. Gen. Microbiol. 43 : 159 - 271 . (10.1099/00221287-43-2-159) / J. Gen. Microbiol. / The aerobic pseudomonads: a taxonomic study by STANIER R. Y. (1966)

Dates

Type	When
Created	5 years, 8 months ago (Jan. 4, 2020, 9:04 p.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:27 p.m.)
Indexed	1 year, 7 months ago (Jan. 11, 2024, 10:57 a.m.)
Issued	57 years, 9 months ago (Dec. 1, 1967)
Published	57 years, 9 months ago (Dec. 1, 1967)
Published Print	57 years, 9 months ago (Dec. 1, 1967)

Funders 0

None

BibTeX

@article{Condon_1967, title={Cold-Sensitive Mutation of Pseudomonas putida Affecting Enzyme Synthesis at Low Temperature}, volume={94}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.94.6.1970-1981.1967}, DOI={10.1128/jb.94.6.1970-1981.1967}, number={6}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Condon, Séamus and Ingraham, John L.}, year={1967}, month=dec, pages={1970–1981} }

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      ]
    ]
  },
  "assertion": [
    {
      "value": "2021-02-01",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}