Reaction of Endotoxin and Surfactants I. Physical and Biological Properties of Endotoxin Treated with Sodium Deoxycholate
10.1128/jb.92.5.1493-1509.1966
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

Ribi, E. (Rocky Mountain Laboratory, Hamilton, Mont.), R. L.Anacker, R. Brown, W. T. Haskins, B. Malmgren, K. C. Milner, and J. A. Rudbach. Reaction of endotoxin and surfactants. I. Physical and biological properties of endotoxin treated with sodium desoxycholate. J. Bacteriol.92:1493–1509. 1966.—Endotoxins from three species of gram-negative bacteria were shown to be dissociated by the bile salt sodium deoxycholate (NaD) into nontoxic subunits with molecular weights of about 20,000. When the bile salt was removed by dialysis, the subunits reaggregated in an orderly manner to form a relatively uniform population of biologically active endotoxin particles with average molecular weights of 500,000 to 1,000,000. If a small amount of human plasma was added to the dissociated endotoxin before removal of the NaD, reassociation apparently did not occur and the preparation remained nonpyrogenic. However, the plasma protein could subsequently be removed from the endotoxin subunits, and reaggregation to the toxic form would then occur. The studies on the physical nature of endotoxin performed with biophysical solution techniques were supplemented and confirmed by direct examination of the endotoxin polymers by electron microscopy. The results of these studies were consonant with the theory that the biologically active endotoxic elements are composed of micellar aggregates of linear lipopolysaccharide subunits.

Bibliography

Ribi, E., Anacker, R. L., Brown, R., Haskins, W. T., Malmgren, B., Milner, K. C., & Rudbach, J. A. (1966). Reaction of Endotoxin and Surfactants I. Physical and Biological Properties of Endotoxin Treated with Sodium Deoxycholate. Journal of Bacteriology, 92(5), 1493–1509.

Authors 7
  1. E. Ribi (first)
  2. R. L. Anacker (additional)
  3. R. Brown (additional)
  4. W. T. Haskins (additional)
  5. B. Malmgren (additional)
  6. K. C. Milner (additional)
  7. J. A. Rudbach (additional)
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Dates
Type When
Created 5 years, 8 months ago (Jan. 4, 2020, 8:56 p.m.)
Deposited 1 year, 11 months ago (Sept. 24, 2023, 2:44 p.m.)
Indexed 1 day, 20 hours ago (Sept. 4, 2025, 10:11 a.m.)
Issued 58 years, 10 months ago (Nov. 1, 1966)
Published 58 years, 10 months ago (Nov. 1, 1966)
Published Print 58 years, 10 months ago (Nov. 1, 1966)
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@article{Ribi_1966, title={Reaction of Endotoxin and Surfactants I. Physical and Biological Properties of Endotoxin Treated with Sodium Deoxycholate}, volume={92}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.92.5.1493-1509.1966}, DOI={10.1128/jb.92.5.1493-1509.1966}, number={5}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Ribi, E. and Anacker, R. L. and Brown, R. and Haskins, W. T. and Malmgren, B. and Milner, K. C. and Rudbach, J. A.}, year={1966}, month=nov, pages={1493–1509} }