Abstract
Welker , N. E. (University of Illinois, Urbana), and L. Leon Campbell . Biochemical changes in lysogenic Bacillus stearothermophilus after bacteriophage induction. J. Bacteriol. 90: 1129–1137. 1965.—Cultures of Bacillus stearothermophilus 1503-4R (TP-1) continued to grow at an unaltered rate after induction with mitomycin C (MC). MC-induced cultures exhibited a 2.5-fold increase in cell number before lysis occurred. Prior to lysis, cells were observed to elongate and to contain areas of lesser density. Protein synthesis was slightly inhibited in MC- or ultraviolet light (UV)-induced cultures for a period of 5 to 10 min, and then proceeded at a rate identical to that in the noninduced culture. Ribonucleic acid (RNA) synthesis was not affected by MC induction. UV induction caused RNA synthesis to occur in two stages: in the first stage, the rate of RNA synthesis was one-third that observed in the noninduced culture and lasted for a period of 15 min; the second stage of RNA synthesis then proceeded at a rate identical to that in the noninduced culture. The synthesis of deoxyribonucleic acid (DNA) in an MC- or UV-induced culture occurred in two stages. In the first stage, DNA synthesis in induced cultures occurred at a rate of one-half (MC) and one-third (UV) of that observed in the noninduced culture. The first stage of DNA synthesis in MC- or UV-induced cultures lasted for 25 to 30 min and 15 to 20 min, respectively. In the second stage, the rate of DNA synthesis in MC- or UV-induced cultures occurred at a rate three times that of the noninduced culture. UV induction appeared to have a greater inhibitory effect than MC induction on protein, RNA, and DNA synthesis as well as phage yield. The differential rate ( K ) of inducible and constitutive α-amylase synthesis was inhibited by 75 and 100%, respectively, for a period of 20 min after MC induction. After 20 min, the K values for α-amylase synthesis were identical to those obtained in the absence of MC induction. The synthesis of TP-1 phage DNA occurred rapidly and was complete 25 min after MC induction, whereas bacterial DNA was degraded or its rate of synthesis was decreased. During the second stage of DNA synthesis, only bacterial DNA was synthesized, but at a rate greater than that found in the noninduced culture.
References
13
Referenced
9
-
BURTON , K. 1956 . Study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid . Biochem. J. 62 : 315 - 323 .
(
10.1042/bj0620315) / Biochem. J. / Study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid by BURTON K. (1956) - DELAPORTE , B. , AND L. SIMINOYITI'CH . 1952 . Recherches cytologiques suir un Bacillius nmegatheriium Iysogene au cours dii developement dii bact 6riophage . Ann. Inst. Pastetur 82 : 90 - 97 . / Ann. Inst. Pastetur / Recherches cytologiques suir un Bacillius nmegatheriium Iysogene au cours dii developement dii bact 6riophage by DELAPORTE B. (1952)
- DISCHE Z. 1955. Color reactions of nuicleic acid components p. 285-305. In E. Chargaff and J. N. Davidson [ed.] The nucleic acids vol. 1. Academic Press Inc. New York.
- IIAYASHIM ., MI . N. HAYASHI , A ND S . SPIEGELMAN. 1963 . Restriction of in vizvo genetic transcription to one of the complementary strands of DNA . Proc. Natl. Acad. Sci. U. S. 50: 664 - 672 . / Proc. Natl. Acad. Sci. U. / Restriction of in vizvo genetic transcription to one of the complementary strands of DNA by IIAYASHIM (1963)
-
LOWRY , 0. H., N. J. ROSEBROUGH , A. L. FARR , A ND R . J. RANDALL. 1951 . Protein measurement with the Folin phenol reagent . J. Biol. Chem. 193 : 265 - 275 .
(
10.1016/S0021-9258(19)52451-6) / J. Biol. Chem. / Protein measurement with the Folin phenol reagent by LOWRY H., N. (1951) -
LWOFF , A. 1953 . Lysogeny . Bacteriol. Rev. 17 : 269 - 337 .
(
10.1128/br.17.4.269-337.1953) / Bacteriol. Rev. / Lysogeny by LWOFF A. (1953) - LWOFF , A., L. SIMINOVIT-CH , A ND N . KJELDGAARD. 1950 . Induction de la productioni de bact 6rio-phages chez une bact 6rie Iysogi.ne . Ann. Inst. Pasteur 79 : 815 - 859 . / Ann. Inst. Pasteur / Induction de la productioni de bact 6rio-phages chez une bact 6rie Iysogi.ne by LWOFF A., L. (1950)
-
'MANDELL , J. A. , AND A. 1). IIERSHEY. 1960 . A fractionating column for analy-sis of nucleic acids . Anal. Biochem. 1 : 66 - 77 .
(
10.1016/0003-2697(60)90020-8) / Anal. Biochem. / A fractionating column for analy-sis of nucleic acids by 'MANDELL J. A. (1960) -
. MARMUR , J. 1961 . A procedure for the isolation of deoxyribonucleic acid from microorganisms . J. Mol. Biol. 3 : 208 - 218 .
(
10.1016/S0022-2836(61)80047-8) / J. Mol. Biol. / A procedure for the isolation of deoxyribonucleic acid from microorganisms by MARMUR J. (1961) -
SIMINOVITCH , L. , AND - S. RAPKINE. 1952 . Biochemical modifications in Iysogenic B. itegatheriunm 899 (1) after indtuction with uiltraviolet light . Biochim. Biophys. Acta 9 : 478 - 487 .
(
10.1016/0006-3002(52)90196-0) / Biochim. Biophys. Acta / Biochemical modifications in Iysogenic B. itegatheriunm 899 (1) after indtuction with uiltraviolet light by SIMINOVITCH L. (1952) -
SUEOKA , N. 1961 . Variation and heterogeneity of base composition of deoxyribonucleic acids: a compilation of old and new data . J. Mol. Biol. 3 : 31 - 40 .
(
10.1016/S0022-2836(61)80005-3) / J. Mol. Biol. / Variation and heterogeneity of base composition of deoxyribonucleic acids: a compilation of old and new data by SUEOKA N. (1961) -
SUEOKA , N. , AND T.-Y. CHENG. 1962 . Fractionation of nucleic acids with the methylated albumin column . J. Mol. Biol. 4 : 161 - 172 .
(
10.1016/S0022-2836(62)80048-5) / J. Mol. Biol. / Fractionation of nucleic acids with the methylated albumin column by SUEOKA N. (1962) - WELKER N. E. AND L. L. CAMPBELL. 1963a. Effect of carbon sources on formation of a-1137
Dates
| Type | When |
|---|---|
| Created | 5 years, 7 months ago (Jan. 3, 2020, 3:50 p.m.) |
| Deposited | 3 years, 11 months ago (Sept. 3, 2021, 9:15 p.m.) |
| Indexed | 1 year, 1 month ago (July 15, 2024, 12:38 p.m.) |
| Issued | 59 years, 10 months ago (Oct. 1, 1965) |
| Published | 59 years, 10 months ago (Oct. 1, 1965) |
| Published Print | 59 years, 10 months ago (Oct. 1, 1965) |
@article{Welker_1965, title={Biochemical Changes in Lysogenic Bacillus stearothermophilus After Bacteriophage Induction}, volume={90}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.90.4.1129-1137.1965}, DOI={10.1128/jb.90.4.1129-1137.1965}, number={4}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Welker, N. E. and Campbell, L. Leon}, year={1965}, month=oct, pages={1129–1137} }