Carbonic anhydrase in Acetobacterium woodii and other acetogenic bacteria
10.1128/jb.179.22.7197-7200.1997
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

Acetobacterium woodii, Acetohalobium arabaticum, Clostridium formicoaceticum, and Sporomusa silvacetica were found to contain carbonic anhydrase (CA). Minimal to no CA activity was detected in Moorella thermoautotrophica, Moorella thermoacetica subsp. "pratumsolum," Sporomusa termitida, and Thermoanaerobacter kivui. Of the acetogens tested, A. woodii had the highest CA specific activity, approximately 14 U mg of protein(-1), in extracts of either glucose- or H2-CO2-cultivated cells. CA of A. woodii was cytoplasmic and was purified approximately 300-fold to a specific activity of 5,236 U mg of protein(-1). Intracellular acetate concentrations inhibited CA activity of A. woodii by 50 to 85%, indicating that intracellular acetate may affect in situ CA activity.

Bibliography

Braus-Stromeyer, S. A., Schnappauf, G., Braus, G. H., Gössner, A. S., & Drake, H. L. (1997). Carbonic anhydrase in Acetobacterium woodii and other acetogenic bacteria. Journal of Bacteriology, 179(22), 7197–7200.

Authors 5
  1. S A Braus-Stromeyer (first)
  2. G Schnappauf (additional)
  3. G H Braus (additional)
  4. A S Gössner (additional)
  5. H L Drake (additional)
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Dates
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Created 8 years, 9 months ago (Nov. 10, 2016, 10:47 a.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 2:07 p.m.)
Indexed 2 months, 3 weeks ago (June 10, 2025, 10:47 p.m.)
Issued 27 years, 10 months ago (Nov. 1, 1997)
Published 27 years, 10 months ago (Nov. 1, 1997)
Published Print 27 years, 10 months ago (Nov. 1, 1997)
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@article{Braus_Stromeyer_1997, title={Carbonic anhydrase in Acetobacterium woodii and other acetogenic bacteria}, volume={179}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.179.22.7197-7200.1997}, DOI={10.1128/jb.179.22.7197-7200.1997}, number={22}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Braus-Stromeyer, S A and Schnappauf, G and Braus, G H and Gössner, A S and Drake, H L}, year={1997}, month=nov, pages={7197–7200} }