DOI: 10.1128/jb.179.20.6522-6524.1997. The Serratia marcescens NucE protein functions as a holin in Escherichia coli

The Serratia marcescens NucE protein functions as a holin in Escherichia coli

10.1128/jb.179.20.6522-6524.1997

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

The recently discovered nucC locus of Serratia marcescens encodes the cryptic prophage genes nucE, nucD, and nucC. NucC is required for expression of the S. marcescens nuclease and functions as a transcriptional activator of the nuclease gene, nucA. NucE and NucD are dispensable for nuclease expression but were proposed to allow for secretion of the nuclease by Escherichia coli. Here, we show (i) that the NucE protein is membrane bound, (ii) that it can complement the lambda S holin, (iii) that it can be triggered by potassium cyanide, (iv) that it is detrimental to cell viability, and (v) that the concomitant expression of nucE and nucD results in cell lysis. Apparently NucE and NucD function as a holin and an endolysin, respectively. This suggests that their roles in nuclease secretion by E. coli are indirect, possibly through directed cell lysis.

Bibliography

Berkmen, M., Benedik, M. J., & BlÃ¤si, U. (1997). The Serratia marcescens NucE protein functions as a holin in Escherichia coli. Journal of Bacteriology, 179(20), 6522â6524.

Authors 3

M Berkmen (first)
M J Benedik (additional)
U Bläsi (additional)

References 13 Referenced 10

Bläsi , U. , and R. Young . 1996 . Two beginnings for a single purpose: the dual-start holins in the regulation of phage Iysis. Mol. Microbiol. 21 : 675 - 682 . (10.1046/j.1365-2958.1996.331395.x) / Iysis. Mol. Microbiol. / Two beginnings for a single purpose: the dual-start holins in the regulation of phage by Bläsi U. (1996)
Fastrez , J. 1996 . Phage Iysozymes . Exper. Suppl. 75 : 35 - 64 . (10.1007/978-3-0348-9225-4_3) / Exper. Suppl. / Phage Iysozymes by Fastrez J. (1996)
Ferrer , S. , M. B. Viejo , J. F. Guasch , J. Enfedaque , and M. Regué . 1996 . Genetic evidence for an activator required for induction of colicin-like bacteriocin 28b production in Serratia marcescens by DNA-damaging agents . J. Bacteriol. 178 : 951 - 960 . (10.1128/jb.178.4.951-960.1996) / J. Bacteriol. / Genetic evidence for an activator required for induction of colicin-like bacteriocin 28b production in Serratia marcescens by DNA-damaging agents by Ferrer S. (1996)
Garrett , J. M. , R. Fusselman , J. Hise , L. Chiou , D. Smith-Grillo , J. Shultz , and R. Young . 1981 . Cell Iysis by induction of cloned lambda Iysis genes . Mol. Gen. Genet. 182 : 326 - 331 . (10.1007/BF00269678) / Mol. Gen. Genet. / Cell Iysis by induction of cloned lambda Iysis genes by Garrett J. M. (1981)
Garrett , J. M. , and R. Young . 1982 . Lethal action of bacteriophageS gene . J. Virol. 44 : 886 - 892 . (10.1128/jvi.44.3.886-892.1982) / J. Virol. / Lethal action of bacteriophageS gene by Garrett J. M. (1982)
Henrich , B. , B. Binishofer , and U. Bläsi . 1995 . Primary structure and functional analysis of the Iysis genes of Lactobacillus gasseri bacteriophage ~adh . J. Bacteriol. 177 : 723 - 732 . (10.1128/jb.177.3.723-732.1995) / J. Bacteriol. / Primary structure and functional analysis of the Iysis genes of Lactobacillus gasseri bacteriophage ~adh by Henrich B. (1995)
Jin , S. , Y. -C. Chen , G. E. Christie , and M. J. Benedik . 1996 . Regulation of the Serratia marcescens extracellular nuclease: positive control by a homolog of P2 Ogr encoded by a cryptic prophage . J. Mol. Biol. 256 : 264 - 278 . (10.1006/jmbi.1996.0084) / J. Mol. Biol. / Regulation of the Serratia marcescens extracellular nuclease: positive control by a homolog of P2 Ogr encoded by a cryptic prophage by Jin S. (1996)
Lee , T. - C. , and E. G. Christie . 1990 . Purification and properties of the bacteriophage P2 ogr gene product: a prokaryotic zinc-binding transcriptional activator . J. Biol. Chem. 265 : 7472 - 7477 . (10.1016/S0021-9258(19)39137-9) / J. Biol. Chem. / Purification and properties of the bacteriophage P2 ogr gene product: a prokaryotic zinc-binding transcriptional activator by Lee T. (1990)
Miller J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.
Steiner , M. , W. Lubitz , and U. Bläsi . 1993 . The missing link in phage Iysis of gram-positive bacteria: gene 14 of Bacillus subtilis phage ~29 encodes the functional homolog of lambda S protein . J. Bacteriol. 175 : 1038 - 1042 . (10.1128/jb.175.4.1038-1042.1993) / J. Bacteriol. / The missing link in phage Iysis of gram-positive bacteria: gene 14 of Bacillus subtilis phage ~29 encodes the functional homolog of lambda S protein by Steiner M. (1993)
Tedin , K. , U. Altanerova , and U. Bläsi . 1995 . Construction of a transducible cassette encoding an inducible bacteriophage T7 RNA polymerase gene 1 in Escherichia coli . J. Microbiol. Methods 21 : 173 - 180 . (10.1016/0167-7012(94)00047-B) / J. Microbiol. Methods / Construction of a transducible cassette encoding an inducible bacteriophage T7 RNA polymerase gene 1 in Escherichia coli by Tedin K. (1995)
Young , R. 1992 . Bacteriophage Iysis: mechanism and regulation . Microbiol. Rev. 56 : 430 - 481 . (10.1128/mr.56.3.430-481.1992) / Microbiol. Rev. / Bacteriophage Iysis: mechanism and regulation by Young R. (1992)
Young , R. , and U. Bläsi . 1995 . Holins: form and function in bacteriophage lysis . FEMS Microbiol. Rev. 17 : 191 - 205 . (10.1016/0168-6445(94)00079-4) / FEMS Microbiol. Rev. / Holins: form and function in bacteriophage lysis by Young R. (1995)

Dates

Type	When
Created	8 years, 9 months ago (Nov. 10, 2016, 10:42 a.m.)
Deposited	4 years ago (July 29, 2021, 2:06 p.m.)
Indexed	1 year, 9 months ago (Oct. 28, 2023, 6:12 p.m.)
Issued	27 years, 10 months ago (Oct. 1, 1997)
Published	27 years, 10 months ago (Oct. 1, 1997)
Published Print	27 years, 10 months ago (Oct. 1, 1997)

Funders 0

None

BibTeX

@article{Berkmen_1997, title={The Serratia marcescens NucE protein functions as a holin in Escherichia coli}, volume={179}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.179.20.6522-6524.1997}, DOI={10.1128/jb.179.20.6522-6524.1997}, number={20}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Berkmen, M and Benedik, M J and Bläsi, U}, year={1997}, month=oct, pages={6522–6524} }

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