Flagellin A is essential for the virulence of Vibrio anguillarum
10.1128/jb.178.5.1310-1319.1996
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

A flagellin gene from the fish pathogen Vibrio anguillarum was cloned, sequenced, and mutagenized. The DNA sequence suggests that the flaA gene encodes a 40.1-kDa protein and is a single transcriptional unit. A polar mutation and four in-frame deletion mutations (180 bp deleted from the 5' end of the gene, 153 bp deleted from the 3' end of the gene, a double deletion of both the 180- and 153-bp deletions, and 942 bp deleted from the entire gene) were made. Compared with the wild type, all mutants were partially motile, and a shortening of the flagellum was seen by electron microscopy. Wild-type phenotypes were regained when the mutations were transcomplemented with the flaA gene. Protein analysis indicated that the flaA gene corresponds to a 40-kDa protein and that the flagellum consists of three additional flagellin proteins with molecular masses of 41, 42, and 45 kDa. N-terminal sequence analysis confirmed that the additional proteins were flagellins with N termini that are 82 to 88% identical to the N terminus of FlaA. Virulence studies showed that the N terminal deletion, the double deletion, and the 942-bp deletion increased the 50% lethal dose between 70- and 700-fold via immersion infection, whereas infection via intraperitoneal injection showed no loss in virulence. In contrast, the polar mutant and the carboxy-terminal deletion mutant showed approximately a 10(4)-fold increase in the 50% lethal dose by both immersion and intraperitoneal infection. In summary, FlaA is needed for crossing the fish integument and may play a role in virulence after invasion of the host.

Bibliography

Milton, D. L., O’Toole, R., Horstedt, P., & Wolf-Watz, H. (1996). Flagellin A is essential for the virulence of Vibrio anguillarum. Journal of Bacteriology, 178(5), 1310–1319.

Authors 4
  1. D L Milton (first)
  2. R O'Toole (additional)
  3. P Horstedt (additional)
  4. H Wolf-Watz (additional)
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Dates
Type When
Created 8 years, 9 months ago (Nov. 10, 2016, 9:23 a.m.)
Deposited 1 year, 2 months ago (June 20, 2024, 10:01 a.m.)
Indexed 2 weeks, 3 days ago (Aug. 12, 2025, 6:11 p.m.)
Issued 29 years, 5 months ago (March 1, 1996)
Published 29 years, 5 months ago (March 1, 1996)
Published Print 29 years, 5 months ago (March 1, 1996)
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@article{Milton_1996, title={Flagellin A is essential for the virulence of Vibrio anguillarum}, volume={178}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.178.5.1310-1319.1996}, DOI={10.1128/jb.178.5.1310-1319.1996}, number={5}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Milton, D L and O’Toole, R and Horstedt, P and Wolf-Watz, H}, year={1996}, month=mar, pages={1310–1319} }