A regulator of the flagellar regulon of Escherichia coli, flhD, also affects cell division
10.1128/jb.178.3.668-674.1996
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

The role of an activator of flagellar transcription in Escherichia coli, flhD, was investigated in the regulation of cell division. When grown in tryptone broth, flhD mutant cells divided exponentially until they reached a cell density of 2.5 x 10(9) cells per ml. Wild-type cells and flhC mutant cells divided exponentially until they reached a cell density of 4 x 10(7) cells per ml. flhD mutant cells divided 5 times more than wild-type cells before they reduced their cell division rate and reached a cell density 37 times higher than that of wild-type or flhC mutant cultures. In stationary phase, the biomasses of all cultures were similar; however, flhD mutant cells were significantly smaller. Additional tryptone, Casamino Acids, and individual amino acids, added at the beginning of growth, allowed wild-type cells to grow to higher cell densities. Serine was determined to have the greatest effect. In contrast, the addition of Casamino Acids did not exhibit an effect upon flhD mutant cells. flhD mutant cells exhibited normal rates of uptake of serine and other amino acids. In both wild-type and flhD mutant cultures, the concentrations of serine in the media dropped from 140 to 20 microM within the first 2 h of growth. Serine concentrations and cell division rates were highly correlated. Wild-type cells reduced their cell division rate at a medium concentration of 50 microM serine, and the addition of serine at this time caused cells to resume a higher rate of division. We conclude that the reduction of the cell division rate in wild-type cells is caused by the depletion of serine from the medium and that flhD mutant cells seem to be unable to sense this depletion.

Bibliography

Prüss, B. M., & Matsumura, P. (1996). A regulator of the flagellar regulon of Escherichia coli, flhD, also affects cell division. Journal of Bacteriology, 178(3), 668–674.

Authors 2
  1. B M Prüss (first)
  2. P Matsumura (additional)
References 34 Referenced 97
  1. Amsler , C. D. , M. Cho , and P. Matsumura . 1993 . Multiple factors underlying the maximum motility of Escherichia coli as cultures enter post-exponential growth . J. Bacteriol. 175 : 6238 - 6244 . (10.1128/JB.175.19.6238-6244.1993) / J. Bacteriol. / Multiple factors underlying the maximum motility of Escherichia coli as cultures enter post-exponential growth by Amsler C. D. (1993)
  2. Bartlett , D. H. , B. B. Frantz , and P. Matsumura . 1988 . Flagellar transcriptional activators FlbB and FlaI: gene sequences and 5~ consensus sequences of operons under FlbB and FlaI control . J. Bacteriol. 170 : 1575 - 1581 . (10.1128/JB.170.4.1575-1581.1988) / J. Bacteriol. / Flagellar transcriptional activators FlbB and FlaI: gene sequences and 5~ consensus sequences of operons under FlbB and FlaI control by Bartlett D. H. (1988)
  3. Brown , T. D. K. , M. C. Jones-Mortimer , and H. L. Kornberg . 1977 . The enzymatic interconversion of acetate and acetyl-coenzyme A in Escherichia coli . J. Gen. Microbiol. 102 : 327 - 336 . (10.1099/00221287-102-2-327) / J. Gen. Microbiol. / The enzymatic interconversion of acetate and acetyl-coenzyme A in Escherichia coli by Brown T. D. K. (1977)
  4. Bryan , R. , D. Glaser , and L. Shapiro . 1990 . Genetic regulatory hierarchy in Caulobacter development . Adv. Genet. 27 : 1 - 31 . (10.1016/S0065-2660(08)60022-X) / Adv. Genet. / Genetic regulatory hierarchy in Caulobacter development by Bryan R. (1990)
  5. Connell N. D. 1989. Regulation of a stationary phase promoter Pmcb in Escherichia coli. Ph.D. thesis. Harvard University Cambridge Mass.
  6. Dingwall , A. , W. Y. Zhuang , K. Quon , and L. Shapiro . 1992 . Expression of an early gene in the flagellar regulatory hierarchy is sensitive to an interruption in DNA replication . J. Bacteriol. 174 : 1760 - 1768 . (10.1128/jb.174.6.1760-1768.1992) / J. Bacteriol. / Expression of an early gene in the flagellar regulatory hierarchy is sensitive to an interruption in DNA replication by Dingwall A. (1992)
  7. Erickson , H. P. 1995 . FtsZ, a procaryotic homolog of tubulin ? Cell 80 : 367 - 370 . (10.1016/0092-8674(95)90486-7) / Cell / FtsZ, a procaryotic homolog of tubulin by Erickson H. P. (1995)
  8. Gibbons J. Personal communication.
  9. Givskov , M. , L. Eberl , G. Christiansen , M. J. Benedik , and S. Molin . 1995 . Induction of phospholipase- and flagellar synthesis in Serratia liquefaciens is controlled by expression of the flagellar master operon flhD . Mol. Microbiol. 15 : 445 - 454 . (10.1111/j.1365-2958.1995.tb02258.x) / Mol. Microbiol. / Induction of phospholipase- and flagellar synthesis in Serratia liquefaciens is controlled by expression of the flagellar master operon flhD by Givskov M. (1995)
  10. Gober , J. W. , and M. V. Marques . 1995 . Regulation of cellular differentiation in Caulobacter crescentus . Microbiol. Rev. 59 : 31 - 47 . (10.1128/mr.59.1.31-47.1995) / Microbiol. Rev. / Regulation of cellular differentiation in Caulobacter crescentus by Gober J. W. (1995)
  11. Helmstetter , C. E. , C. Eenhuis , P. Theisen , J. Grimwalde , and A. C. Leonard . 1992 . Improved bacterial baby machine: application to Escherichia coli K-12 . J. Bacteriol. 174 : 3445 - 3449 . (10.1128/jb.174.11.3445-3449.1992) / J. Bacteriol. / Improved bacterial baby machine: application to Escherichia coli K-12 by Helmstetter C. E. (1992)
  12. Holms , W. H. 1986 . The central metabolic pathways of Escherichia coli: relationship between flux and control at a branch point, efficiency of conversion to biomass, and excretion of acetate . Curr. Top. Cell. Regul. 28 : 69 - 105 . (10.1016/B978-0-12-152828-7.50004-4) / Curr. Top. Cell. Regul. / The central metabolic pathways of Escherichia coli: relationship between flux and control at a branch point, efficiency of conversion to biomass, and excretion of acetate by Holms W. H. (1986)
  13. Kawamukai , M. , H. Matsuda , W. Fujii , R. Utsumi , and T. Komano . 1989 . Nucleotide sequences of fic and fic-1 genes involved in cell filamentation induced by cyclic AMP in Escherichia coli . J. Bacteriol. 171 : 4525 - 4529 . (10.1128/jb.171.8.4525-4529.1989) / J. Bacteriol. / Nucleotide sequences of fic and fic-1 genes involved in cell filamentation induced by cyclic AMP in Escherichia coli by Kawamukai M. (1989)
  14. Kim M. C. 1989. Molecular characterization and regulation of the gene expression of flhB operon in Escherichia coli. Ph.D. thesis. University of Illinois Chicago.
  15. Komeda , Y. , and T. Iino . 1979 . Regulation of expression of the flagellin gene (hag) in Escherichia coli K-12: analysis of hag-lac fusions . J. Bacteriol. 139 : 721 - 729 . (10.1128/JB.139.3.721-729.1979) / J. Bacteriol. / Regulation of expression of the flagellin gene (hag) in Escherichia coli K-12: analysis of hag-lac fusions by Komeda Y. (1979)
  16. Komeda , Y. , K. Katsukake , and T. Iino . 1980 . Definition of additional flagellar genes in Escherichia coli K-12 . Genetics 94 : 277 - 290 . (10.1093/genetics/94.2.277) / Genetics / Definition of additional flagellar genes in Escherichia coli K-12 by Komeda Y. (1980)
  17. Liu , X. , and P. Matsumura . 1994 . The FlhD/FlhC complex, a transcriptional activator of the Escherichia coli flagellar class II operons . J. Bacteriol. 176 : 7345 - 7351 . (10.1128/JB.176.23.7345-7351.1994) / J. Bacteriol. / The FlhD/FlhC complex, a transcriptional activator of the Escherichia coli flagellar class II operons by Liu X. (1994)
  18. Liu , X. , and P. Matsumura . 1995 . An alternative sigma factor controls transcription of flagellar class-III operons in Escherichia coli: gene sequence, overproduction, purification and characterization . Gene 164 : 810 - 884 . / Gene / An alternative sigma factor controls transcription of flagellar class-III operons in Escherichia coli: gene sequence, overproduction, purification and characterization by Liu X. (1995)
  19. Macnab , R. M. 1992 . Genetics and biogenesis of bacterial flagella. Annu . Rev. Genet. 26 : 131 - 158 . (10.1146/annurev.ge.26.120192.001023) / Rev. Genet. / Genetics and biogenesis of bacterial flagella. Annu by Macnab R. M. (1992)
  20. Malakooti J. 1989. Molecular analysis of the Escherichia coli motor components and regulation of internal control elements of the flaA operon. Ph.D. thesis. University of Illinois Chicago.
  21. McCleary , W. R. , J. B. Stock , and A. J. Ninfa . 1993 . Is acetyl phosphate a global signal in Escherichia coli ? J. Bacteriol. 175 : 2793 - 2798 . (10.1128/JB.175.10.2793-2798.1993) / J. Bacteriol. / Is acetyl phosphate a global signal in Escherichia coli by McCleary W. R. (1993)
  22. Miller J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.
  23. Nishimura , A. , and Y. Hirota . 1989 . A cell division regulatory mechanism controls the flagellar regulon in Escherichia coli . Mol. Gen. Genet. 216 : 340 - 346 . (10.1007/BF00334374) / Mol. Gen. Genet. / A cell division regulatory mechanism controls the flagellar regulon in Escherichia coli by Nishimura A. (1989)
  24. Prüß B. M. and P. Matsumura. Unpublished data.
  25. Prüß , B. M. , J. M. Nelms , C. Park , and A. J. Wolfe . 1994 . Mutations in NADH:ubiquinone oxidoreductase of Escherichia coli affect growth on mixed amino acids . J. Bacteriol. 176 : 2143 - 2150 . (10.1128/JB.176.8.2143-2150.1994) / J. Bacteriol. / Mutations in NADH:ubiquinone oxidoreductase of Escherichia coli affect growth on mixed amino acids by Prüß B. M. (1994)
  26. Prüß , B. M. , and A. J. Wolfe . 1994 . Regulation of acetyl phosphate synthesis and degradation, and the control of flagellar expression in Escherichia coli . Mol. Microbiol. 12 : 973 - 984 . (10.1111/j.1365-2958.1994.tb01085.x) / Mol. Microbiol. / Regulation of acetyl phosphate synthesis and degradation, and the control of flagellar expression in Escherichia coli by Prüß B. M. (1994)
  27. Robinson , A. C. , J. F. Collins , and W. D. Donachie . 1987 . Procaryotic and eucaryotic cell-cycle proteins . Nature (London) 328 : 766 . (10.1038/328766a0) / Nature (London) / Procaryotic and eucaryotic cell-cycle proteins by Robinson A. C. (1987)
  28. Shi , W. , Y. Zhou , J. Wild , J. Adler , and C. A. Gross . 1992 . DnaK, DnaJ, and GrpE are required for flagellum synthesis in Escherichia coli . J. Bacteriol. 174 : 6256 - 6263 . (10.1128/jb.174.19.6256-6263.1992) / J. Bacteriol. / DnaK, DnaJ, and GrpE are required for flagellum synthesis in Escherichia coli by Shi W. (1992)
  29. Shin , S. , and C. Park . 1995 . Modulation of flagellar expression in Escherichia coli by acetyl phosphate and the osmoregulator OmpR . J. Bacteriol. 177 : 4696 - 4702 . (10.1128/JB.177.16.4696-4702.1995) / J. Bacteriol. / Modulation of flagellar expression in Escherichia coli by acetyl phosphate and the osmoregulator OmpR by Shin S. (1995)
  30. Silhavy T. J. M. L. Berman and L. W. Enquist. 1984. Experiments with gene fusions. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.
  31. Silverman , M. , and M. Simon . 1974 . Characterization of Escherichia coli flagellar mutants that are insensitive to catabolite repression . J. Bacteriol. 120 : 1196 - 1203 . (10.1128/JB.120.3.1196-1203.1974) / J. Bacteriol. / Characterization of Escherichia coli flagellar mutants that are insensitive to catabolite repression by Silverman M. (1974)
  32. Tentler S. 1994. Gene regulation within the flhB operon of Escherichia coli. M.S. thesis. University of Illinois Chicago.
  33. Yanisch-Perron , C. , J. Vieira , and J. Messing . 1985 . Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors . Gene 33 : 103 - 119 . (10.1016/0378-1119(85)90120-9) / Gene / Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors by Yanisch-Perron C. (1985)
  34. Yu , J. , and L. Shapiro . 1992 . Early Caulobacter crescentus genes fliL and fliM are required for flagellar gene expression and normal cell division . J. Bacteriol. 174 : 3327 - 3338 . (10.1128/jb.174.10.3327-3338.1992) / J. Bacteriol. / Early Caulobacter crescentus genes fliL and fliM are required for flagellar gene expression and normal cell division by Yu J. (1992)
Dates
Type When
Created 8 years, 9 months ago (Nov. 10, 2016, 9:07 a.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 2 p.m.)
Indexed 4 months ago (April 29, 2025, 9:32 a.m.)
Issued 29 years, 6 months ago (Feb. 1, 1996)
Published 29 years, 6 months ago (Feb. 1, 1996)
Published Print 29 years, 6 months ago (Feb. 1, 1996)
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@article{Pr_ss_1996, title={A regulator of the flagellar regulon of Escherichia coli, flhD, also affects cell division}, volume={178}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.178.3.668-674.1996}, DOI={10.1128/jb.178.3.668-674.1996}, number={3}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Prüss, B M and Matsumura, P}, year={1996}, month=feb, pages={668–674} }