FtsA is localized to the septum in an FtsZ-dependent manner
10.1128/jb.178.24.7167-7172.1996
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

The localization of the cell division protein FtsA in E. coli was examined. FtsA was found to localize to the septum in a ring pattern as previously shown for FtsZ. The localization of FtsA was completely dependent on the localization of FtsZ. Under a variety of conditions that prevented formation of the Z ring, FtsA was unable to localize. In mutants where FtsZ forms structures in addition to Z rings, the pattern of FtsA duplicated these structures. These results suggest that the Z ring recruits FtsA to the septum.

Bibliography

Addinall, S. G., & Lutkenhaus, J. (1996). FtsA is localized to the septum in an FtsZ-dependent manner. Journal of Bacteriology, 178(24), 7167–7172.

Authors 2
  1. S G Addinall (first)
  2. J Lutkenhaus (additional)
References 37 Referenced 156
  1. Addinall , S. G. , E. Bi , and J. Lutkenhaus . 1996 . FtsZ ring formation in fts mutants . J. Bacteriol. 178 : 3877 - 3884 . (10.1128/JB.178.13.3877-3884.1996) / J. Bacteriol. / FtsZ ring formation in fts mutants by Addinall S. G. (1996)
  2. Addinall , S. G. , and J. Lutkenhaus . 1996 . FtsZ spirals and arcs determine the shape of the invaginating septa in some mutants of Escherichia coli . Mol. Microbiol. 22 : 231 - 237 . (10.1046/j.1365-2958.1996.00100.x) / Mol. Microbiol. / FtsZ spirals and arcs determine the shape of the invaginating septa in some mutants of Escherichia coli by Addinall S. G. (1996)
  3. Addinall S. G. and J. Lutkenhaus. 1996. Unpublished data.
  4. Beall , B. , and J. Lutkenhaus . 1992 . Impaired cell division and sporulation of a Bacillus subtilis strain with the ftsA gene deleted . J. Bacteriol. 174 : 2398 - 2403 . (10.1128/jb.174.7.2398-2403.1992) / J. Bacteriol. / Impaired cell division and sporulation of a Bacillus subtilis strain with the ftsA gene deleted by Beall B. (1992)
  5. Begg , K. J. , and W. D. Donachie . 1985 . Cell shape and division in Escherichia coli: experiments with shape and division mutants . J. Bacteriol. 163 : 615 - 622 . (10.1128/JB.163.2.615-622.1985) / J. Bacteriol. / Cell shape and division in Escherichia coli: experiments with shape and division mutants by Begg K. J. (1985)
  6. Begg , K. J. , A. Takasuga , D. H. Edwards , S. J. Dewar , B. G. Spratt , and W. D. Donachie . 1990 . The balance between different peptidoglycan precursors determines whether Escherichia coli cells will elongate or divide . J. Bacteriol. 172 : 6697 - 6703 . (10.1128/JB.172.12.6697-6703.1990) / J. Bacteriol. / The balance between different peptidoglycan precursors determines whether Escherichia coli cells will elongate or divide by Begg K. J. (1990)
  7. Bi , E. , and J. Lutkenhaus . 1991 . FtsZ ring structure associated with division in Escherichia coli . Nature (London) 354 : 161 - 164 . (10.1038/354161a0) / Nature (London) / FtsZ ring structure associated with division in Escherichia coli by Bi E. (1991)
  8. Bi E. and J. Lutkenhaus. 1992. Genetics of bacterial cell division p. 123-152. In S. Mohan C. Dow and J. A. Cole (ed.) Prokaryotic structure and function: a new perspective. Cambridge University Press Cambridge.
  9. Bi , E. , and J. Lutkenhaus . 1992 . Isolation and characterization of ftsZ alleles that affect septal morphology . J. Bacteriol. 174 : 5414 - 5423 . (10.1128/jb.174.16.5414-5423.1992) / J. Bacteriol. / Isolation and characterization of ftsZ alleles that affect septal morphology by Bi E. (1992)
  10. Bi , E. , and J. Lutkenhaus . 1993 . Cell division inhibitors SulA and MinCD prevent formation of the FtsZ ring . J. Bacteriol. 175 : 1118 - 1125 . (10.1128/jb.175.4.1118-1125.1993) / J. Bacteriol. / Cell division inhibitors SulA and MinCD prevent formation of the FtsZ ring by Bi E. (1993)
  11. Bork , P. , C. Sander , and A. Valencia . 1992 . An ATPase domain common to prokaryotic cell cycle proteins, sugar kinases, actin and hsp70 heat shock proteins . Proc. Natl. Acad. Sci. USA 89 : 7290 - 7294 . (10.1073/pnas.89.16.7290) / Proc. Natl. Acad. Sci. USA / An ATPase domain common to prokaryotic cell cycle proteins, sugar kinases, actin and hsp70 heat shock proteins by Bork P. (1992)
  12. Bramhill , D. , and C. M. Thompson . 1994 . GTP-dependent polymerization of Escherichia coli FtsZ protein to form tubules . Proc. Natl. Acad. Sci. USA 91 : 5813 - 5817 . (10.1073/pnas.91.13.5813) / Proc. Natl. Acad. Sci. USA / GTP-dependent polymerization of Escherichia coli FtsZ protein to form tubules by Bramhill D. (1994)
  13. Cao C. L. Wang A. Mukherjee and J. Lutkenhaus. 1996. Unpublished data.
  14. Casadaban , M. 1976 . Transposition and fusion of the lac gene to selected promoters in E. coli using bacteriophageand Mu . J. Mol. Biol. 104 : 541 - 555 . (10.1016/0022-2836(76)90119-4) / J. Mol. Biol. / Transposition and fusion of the lac gene to selected promoters in E. coli using bacteriophageand Mu by Casadaban M. (1976)
  15. Dai K. 1992. Characterization of cell division genes of E. coli. Ph.D. thesis. University of Kansas Kansas City.
  16. Dai , K. , and J. Lutkenhaus . 1991 . ftsZ is an essential cell division gene in Escherichia coli . J. Bacteriol. 173 : 3500 - 3506 . (10.1128/JB.173.11.3500-3506.1991) / J. Bacteriol. / ftsZ is an essential cell division gene in Escherichia coli by Dai K. (1991)
  17. Dai , K. , and J. Lutkenhaus . 1992 . The proper ratio of FtsZ to FtsA is required for cell division to occur in Escherichia coli . J. Bacteriol. 174 : 6145 - 6151 . (10.1128/jb.174.19.6145-6151.1992) / J. Bacteriol. / The proper ratio of FtsZ to FtsA is required for cell division to occur in Escherichia coli by Dai K. (1992)
  18. Dai , K. , Y. Xu , and J. Lutkenhaus . 1993 . Cloning and characterization of ftsN, an essential cell division gene in Escherichia coli, isolated as a multicopy suppressor of ftsA12(Ts) . J. Bacteriol. 175 : 3790 - 3797 . (10.1128/JB.175.12.3790-3797.1993) / J. Bacteriol. / Cloning and characterization of ftsN, an essential cell division gene in Escherichia coli, isolated as a multicopy suppressor of ftsA12(Ts) by Dai K. (1993)
  19. Dai , K. , Y. Xu , and J. Lutkenhaus . 1995 . Topological characterization of the essential E. coli cell division protein FtsN . J. Bacteriol. 175 : 3790 - 3797 . (10.1128/JB.175.12.3790-3797.1993) / J. Bacteriol. / Topological characterization of the essential E. coli cell division protein FtsN by Dai K. (1995)
  20. de Boer , P. A. J. , W. R. Cook , and L. I. Rothfield . 1990 . Bacterial cell division. Annu . Rev. Genet. 24 : 249 - 274 . (10.1146/annurev.ge.24.120190.001341) / Rev. Genet. / Bacterial cell division. Annu by de Boer P. A. J. (1990)
  21. Dewar , S. J. , K. J. Begg , and W. D. Donachie . 1992 . Inhibition of cell division initiation by an imbalance in the ratio of FtsA to FtsZ . J. Bacteriol. 174 : 6314 - 6316 . (10.1128/jb.174.19.6314-6316.1992) / J. Bacteriol. / Inhibition of cell division initiation by an imbalance in the ratio of FtsA to FtsZ by Dewar S. J. (1992)
  22. Donachie , W. D. 1993 . The cell cycle of Escherichia coli. Annu . Rev. Microbiol. 47 : 199 - 230 . (10.1146/annurev.mi.47.100193.001215) / Rev. Microbiol. / The cell cycle of Escherichia coli. Annu by Donachie W. D. (1993)
  23. Erickson , H. P. , D. W. Taylor , K. A. Taylor , and D. Bramhill . 1996 . Bacterial cell division protein FtsZ assembles into protofilament sheet and minirings, structural homologs of tubulin polymers . Proc. Natl. Acad. Sci. USA 93 : 519 - 523 . (10.1073/pnas.93.1.519) / Proc. Natl. Acad. Sci. USA / Bacterial cell division protein FtsZ assembles into protofilament sheet and minirings, structural homologs of tubulin polymers by Erickson H. P. (1996)
  24. Guzman , L. - Z. , D. Belin , M. J. Carson , and J. Beckwith . 1995 . Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter . J. Bacteriol. 177 : 4121 - 4130 . (10.1128/JB.177.14.4121-4130.1995) / J. Bacteriol. / Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter by Guzman L. (1995)
  25. Higashitani , A. , N. Higashitani , and K. Horiuchi . 1995 . A cell division inhibitor SulA of Escherichia coli directly interacts with FtsZ through GTP hydrolysis . Biochem. Biophys. Res. Commun. 209 : 198 - 204 . (10.1006/bbrc.1995.1489) / Biochem. Biophys. Res. Commun. / A cell division inhibitor SulA of Escherichia coli directly interacts with FtsZ through GTP hydrolysis by Higashitani A. (1995)
  26. Huang , J. , C. Cao , and J. Lutkenhaus . 1996 . Interaction between FtsZ and inhibitors of cell division . J. Bacteriol. 178 : 5080 - 5085 . (10.1128/jb.178.17.5080-5085.1996) / J. Bacteriol. / Interaction between FtsZ and inhibitors of cell division by Huang J. (1996)
  27. Levin , P. A. , and R. Losick . 1996 . Transcription factor SpoOA switches the localization of the cell division protein FtsZ from a medial to a bipolar pattern in Bacillus subtilis . Genes Dev. 10 : 478 - 488 . (10.1101/gad.10.4.478) / Genes Dev. / Transcription factor SpoOA switches the localization of the cell division protein FtsZ from a medial to a bipolar pattern in Bacillus subtilis by Levin P. A. (1996)
  28. Lutkenhaus , J. 1993 . FtsZ ring in bacterial cytokinesis . Mol. Microbiol. 9 : 404 - 409 . (10.1111/j.1365-2958.1993.tb01701.x) / Mol. Microbiol. / FtsZ ring in bacterial cytokinesis by Lutkenhaus J. (1993)
  29. Miller J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.
  30. Mukherjee , A. , and J. Lutkenhaus . 1994 . Guanine nucleotide assembly of FtsZ into filaments . J. Bacteriol. 176 : 2754 - 2758 . (10.1128/JB.176.9.2754-2758.1994) / J. Bacteriol. / Guanine nucleotide assembly of FtsZ into filaments by Mukherjee A. (1994)
  31. Sanchez , M. , A. Valencia , M. -J. Ferrandiz , C. Sander , and M. Vicente . 1994 . Corelation between the structure and biochemical activities of FtsA, an essential cell division protein of the actin family . EMBO J. 13 : 4919 - 4925 . (10.1002/j.1460-2075.1994.tb06819.x) / EMBO J. / Corelation between the structure and biochemical activities of FtsA, an essential cell division protein of the actin family by Sanchez M. (1994)
  32. Taschner , P. E. , P. G. Huls , E. Pas , and C. L. Woldringh . 1988 . Division behavior and shape changes in isogenic ftsZ, ftsQ, ftsA, pbpB, and ftsE cell division mutants of Escherichia coli during temperature shift experiments . J. Bacteriol. 170 : 1533 - 1540 . (10.1128/jb.170.4.1533-1540.1988) / J. Bacteriol. / Division behavior and shape changes in isogenic ftsZ, ftsQ, ftsA, pbpB, and ftsE cell division mutants of Escherichia coli during temperature shift experiments by Taschner P. E. (1988)
  33. Tormo , A. , J. A. Ayala , M. A. de Pedro , and M. Vicente . 1986 . Interaction of FtsA and PBP3 proteins in the Escherichia coli septum . J. Bacteriol. 166 : 985 - 992 . (10.1128/jb.166.3.985-992.1986) / J. Bacteriol. / Interaction of FtsA and PBP3 proteins in the Escherichia coli septum by Tormo A. (1986)
  34. Tormo , A. , and M. Vicente . 1984 . The ftsA gene product participates in formation of the Escherichia coli septum structure . J. Bacteriol. 157 : 779 - 784 . (10.1128/jb.157.3.779-784.1984) / J. Bacteriol. / The ftsA gene product participates in formation of the Escherichia coli septum structure by Tormo A. (1984)
  35. Vieira , J. , and J. Messing . 1982 . The pUC plasmids, an M13mp7 derived system for insertion mutagenesis and sequencing with synthetic universal primers . Gene 19 : 259 - 268 . (10.1016/0378-1119(82)90015-4) / Gene / The pUC plasmids, an M13mp7 derived system for insertion mutagenesis and sequencing with synthetic universal primers by Vieira J. (1982)
  36. Wang , H. , and R. C. Gayda . 1992 . Quantitative determination of FtsA at different growth rates in Escherichia coli using monoclonal antibodies . Mol. Microbiol. 6 : 2517 - 2524 . (10.1111/j.1365-2958.1992.tb01428.x) / Mol. Microbiol. / Quantitative determination of FtsA at different growth rates in Escherichia coli using monoclonal antibodies by Wang H. (1992)
  37. Ward , J. E. , Jr. , and J. Lutkenhaus . 1985 . Overproduction of FtsZ induces minicell formation in Escherichia coli . Cell 42 : 941 - 949 . (10.1016/0092-8674(85)90290-9) / Cell / Overproduction of FtsZ induces minicell formation in Escherichia coli by Ward J. E. (1985)
Dates
Type When
Created 8 years, 9 months ago (Nov. 10, 2016, 8:37 a.m.)
Deposited 4 years ago (July 29, 2021, 1:59 p.m.)
Indexed 3 weeks, 1 day ago (Aug. 6, 2025, 8:05 a.m.)
Issued 28 years, 8 months ago (Dec. 1, 1996)
Published 28 years, 8 months ago (Dec. 1, 1996)
Published Print 28 years, 8 months ago (Dec. 1, 1996)
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@article{Addinall_1996, title={FtsA is localized to the septum in an FtsZ-dependent manner}, volume={178}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.178.24.7167-7172.1996}, DOI={10.1128/jb.178.24.7167-7172.1996}, number={24}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Addinall, S G and Lutkenhaus, J}, year={1996}, month=dec, pages={7167–7172} }