Characterization of the recA gene regions of Spiroplasma citri and Spiroplasma melliferum
10.1128/jb.178.23.7003-7009.1996
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

In previous studies (A. Marais, J. M. Bove, and J. Renaudin, J. Bacteriol. 178:862-870, 1996), we have shown that the recA gene of Spiroplasma citri R8A2 was restricted to the first 390 nucleotides of the N-terminal part. PCR amplification and sequencing studies of five additional strains of S. citri have revealed that these strains had the same organization at the recA region as the R8A2 strain. In contrast to S. citri, Spiroplasma melliferum was found to contain a full-length recA gene. However, in all five S. melliferum strains tested, a TAA stop codon was found within the N-terminal region of the recA reading frame. Our results suggest that S. melliferum, as well as S. citri, is RecA deficient. In agreement with the recA mutant genotype of S. citri and S. melliferum, we have shown that these organisms are highly sensitive to UV irradiation.

Bibliography

Marais, A., Bove, J. M., & Renaudin, J. (1996). Characterization of the recA gene regions of Spiroplasma citri and Spiroplasma melliferum. Journal of Bacteriology, 178(23), 7003–7009.

Authors 3
  1. A Marais (first)
  2. J M Bove (additional)
  3. J Renaudin (additional)
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Created 8 years, 9 months ago (Nov. 10, 2016, 8:42 a.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 2 p.m.)
Indexed 1 month ago (July 30, 2025, 8:34 p.m.)
Issued 28 years, 9 months ago (Dec. 1, 1996)
Published 28 years, 9 months ago (Dec. 1, 1996)
Published Print 28 years, 9 months ago (Dec. 1, 1996)
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@article{Marais_1996, title={Characterization of the recA gene regions of Spiroplasma citri and Spiroplasma melliferum}, volume={178}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.178.23.7003-7009.1996}, DOI={10.1128/jb.178.23.7003-7009.1996}, number={23}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Marais, A and Bove, J M and Renaudin, J}, year={1996}, month=dec, pages={7003–7009} }