DOI: 10.1128/jb.178.19.5776-5780.1996. Sulfur-binding protein of flagella of Thiobacillus ferrooxidans

Sulfur-binding protein of flagella of Thiobacillus ferrooxidans

10.1128/jb.178.19.5776-5780.1996

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

The sulfur-binding protein of Thiobacillus ferrooxidans ATCC 23270 was investigated. The protein composition of the bacterium's cell surface changed according to the culture substrate. Sulfur-grown cells showed greater adhesion to sulfur than iron-grown cells. The sulfur-grown cells synthesized a 40-kDa surface protein which was not synthesized by iron-grown cells. The 40-kDa protein had thiol groups and strongly adhered to elemental sulfur powder. This adhesion was not disturbed by Triton X-100, which can quench hydrophobic interactions. However, adhesion was disturbed by 2-mercaptoethanol, which broke the disulfide bond. The thiol groups of the 40-kDa protein formed a disulfide bond with elemental sulfur and mediated the strong adhesion between T. ferrooxidans cells and elemental sulfur. The 40-kDa protein was located on the flagella. The location of the protein would make it possible for cells to be in closer contact with the surface of elemental sulfur powder.

Bibliography

Ohmura, N., Tsugita, K., Koizumi, J. I., & Saika, H. (1996). Sulfur-binding protein of flagella of Thiobacillus ferrooxidans. Journal of Bacteriology, 178(19), 5776â5780.

Authors 4

N Ohmura (first)
K Tsugita (additional)
J I Koizumi (additional)
H Saika (additional)

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Dates

Type	When
Created	8 years, 9 months ago (Nov. 10, 2016, 8:12 a.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:58 p.m.)
Indexed	2 months, 3 weeks ago (June 10, 2025, 4:26 a.m.)
Issued	28 years, 11 months ago (Oct. 1, 1996)
Published	28 years, 11 months ago (Oct. 1, 1996)
Published Print	28 years, 11 months ago (Oct. 1, 1996)

Funders 0

None

BibTeX

@article{Ohmura_1996, title={Sulfur-binding protein of flagella of Thiobacillus ferrooxidans}, volume={178}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.178.19.5776-5780.1996}, DOI={10.1128/jb.178.19.5776-5780.1996}, number={19}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Ohmura, N and Tsugita, K and Koizumi, J I and Saika, H}, year={1996}, month=oct, pages={5776–5780} }

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