Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

Xcp proteins constitute the secretory apparatus of Pseudomonas aeruginosa. Deduced amino acid sequence of xcp genes, expression, and subcellular localization revealed unexpected features. Indeed, most Xcp proteins are found in the cytoplasmic membrane although xcp mutations lead to periplasmic accumulation of exoproteins, indicating that the limiting step is translocation across the outer membrane. To understand the mechanism by which the machinery functions and the interactions between its components, it is valuable to know their membrane organization. We report data demonstrating the N(in)-C(out) topologies of three general secretion pathway components, the XcpP, -Y, and -Z proteins.

Bibliography

Bleves, S., Lazdunski, A., & Filloux, A. (1996). Membrane topology of three Xcp proteins involved in exoprotein transport by Pseudomonas aeruginosa. Journal of Bacteriology, 178(14), 4297–4300.

Authors 3
  1. S Bleves (first)
  2. A Lazdunski (additional)
  3. A Filloux (additional)
References 21 Referenced 38
  1. Akrim , M. , M. Bally , G. Ball , J. Tommassen , H. Teerink , A. Filloux , and A. Lazdunski . 1993 . Xcp-mediated protein secretion in Pseudomonas aeruginosa: identification of two additional genes and evidence for regulation of xcp gene expression . Mol. Microbiol. 10 : 431 - 443 . (10.1111/j.1365-2958.1993.tb02674.x) / Mol. Microbiol. / Xcp-mediated protein secretion in Pseudomonas aeruginosa: identification of two additional genes and evidence for regulation of xcp gene expression by Akrim M. (1993)
  2. Bally , M. , A. Filloux , M. Akrim , G. Ball , A. Lazdunski , and J. Tommassen . 1992 . Processing of components of the secretory apparatus of Pseudomonas aeruginosa by prepilin peptidase . Mol. Microbiol. 6 : 1121 - 1131 . (10.1111/j.1365-2958.1992.tb01550.x) / Mol. Microbiol. / Processing of components of the secretory apparatus of Pseudomonas aeruginosa by prepilin peptidase by Bally M. (1992)
  3. Broome-Smith , J. , and B. G. Spratt . 1986 . A vector for the construction of translational fusions to the TEM ~-lactamase and analysis of protein export signals and membrane protein topology . Gene 49 : 341 - 349 . (10.1016/0378-1119(86)90370-7) / Gene / A vector for the construction of translational fusions to the TEM ~-lactamase and analysis of protein export signals and membrane protein topology by Broome-Smith J. (1986)
  4. de Groot , A. , A. Filloux , and J. Tommassen . 1991 . Conservation of xcp genes, involved in the two-step secretion process, in different Pseudomonas species and other Gram-negative bacteria . Mol. Gen. Genet. 229 : 278 - 284 . (10.1007/BF00272167) / Mol. Gen. Genet. / Conservation of xcp genes, involved in the two-step secretion process, in different Pseudomonas species and other Gram-negative bacteria by de Groot A. (1991)
  5. d' Enfert , C. , I. Reyss , C. Wandersman , and A. P. Pugsley . 1989 . Protein secretion by Gram-negative bacteria. Characterization of two membrane proteins required for pullulanase secretion by Escherichia coli K12 . J. Biol. Chem. 264 : 17462 - 17468 . / J. Biol. Chem. / Protein secretion by Gram-negative bacteria. Characterization of two membrane proteins required for pullulanase secretion by Escherichia coli K12 by Enfert C. (1989)
  6. Filloux , A. , M. Bally , G. Ball , M. Akrim , J. Tommassen , and A. Lazdunski . 1990 . Protein secretion in Gram-negative bacteria: transport across the outer membrane involves common mechanism in different bacteria . EMBO J. 9 : 4323 - 4329 . (10.1002/j.1460-2075.1990.tb07881.x) / EMBO J. / Protein secretion in Gram-negative bacteria: transport across the outer membrane involves common mechanism in different bacteria by Filloux A. (1990)
  7. Gutierrez , C. , and J. C. Devedjian . 1989 . A plasmid facilitating in vitro construction of phoA gene fusions in Escherichia coli . Nucleic Acids Res. 17 : 3999 . (10.1093/nar/17.10.3999) / Nucleic Acids Res. / A plasmid facilitating in vitro construction of phoA gene fusions in Escherichia coli by Gutierrez C. (1989)
  8. Hoffman , C. S. , and A. Wright . 1985 . Fusions of secreted proteins to alkaline phosphatase: an approach for studying protein secretion . Proc. Natl. Acad. Sci. USA 82 : 5107 - 5111 . (10.1073/pnas.82.15.5107) / Proc. Natl. Acad. Sci. USA / Fusions of secreted proteins to alkaline phosphatase: an approach for studying protein secretion by Hoffman C. S. (1985)
  9. Liu , P. V. 1974 . Extracellular toxins of Pseudomonas aeruginosa . J. Infect. Dis. 130 : 94 - 99 . (10.1093/infdis/130.Supplement.S94) / J. Infect. Dis. / Extracellular toxins of Pseudomonas aeruginosa by Liu P. V. (1974)
  10. Nunn , D. , S. Bergman , and S. Lory . 1993 . Products of three accessory genes, pilB, pilC, and pilD, are required for biogenesis of Pseudomonas aeruginosa pili . J. Bacteriol. 172 : 2911 - 2919 . (10.1128/JB.172.6.2911-2919.1990) / J. Bacteriol. / Products of three accessory genes, pilB, pilC, and pilD, are required for biogenesis of Pseudomonas aeruginosa pili by Nunn D. (1993)
  11. Pugsley , A. P. 1993 . The complete general secretory pathway in gram-negative bacteria . Microbiol. Rev. 57 : 50 - 108 . (10.1128/mr.57.1.50-108.1993) / Microbiol. Rev. / The complete general secretory pathway in gram-negative bacteria by Pugsley A. P. (1993)
  12. Pugsley , A. P. , and I. Reyss . 1990 . Five genes at the 3~ end of the Klebsiella pneumoniae pulC operon are required for pullulanase secretion . Mol. Microbiol. 4 : 365 - 379 . (10.1111/j.1365-2958.1990.tb00604.x) / Mol. Microbiol. / Five genes at the 3~ end of the Klebsiella pneumoniae pulC operon are required for pullulanase secretion by Pugsley A. P. (1990)
  13. Reeves , P. J. , P. Douglas , and G. P. C. Salmond . 1994 . Beta-lactamase topology probe analysis of the OutO NMePhE peptidase, and six other Out protein components of the Erwinia carotovora general secretion pathway apparatus . Mol. Microbiol. 12 : 445 - 457 . (10.1111/j.1365-2958.1994.tb01033.x) / Mol. Microbiol. / Beta-lactamase topology probe analysis of the OutO NMePhE peptidase, and six other Out protein components of the Erwinia carotovora general secretion pathway apparatus by Reeves P. J. (1994)
  14. Sandkvist , M. , M. Bagdasarian , S. P. Howard , and V. J. DiRita . 1995 . Interaction between the autokinase EpsE and EpsL in the cytoplasmic membrane is required for extracellular secretion in Vibrio cholerae . EMBO J. 14 : 1664 - 1673 . (10.1002/j.1460-2075.1995.tb07155.x) / EMBO J. / Interaction between the autokinase EpsE and EpsL in the cytoplasmic membrane is required for extracellular secretion in Vibrio cholerae by Sandkvist M. (1995)
  15. Schatz , P. J. , and J. Beckwith . 1990 . Genetic analysis of protein export in Escherichia coli. Annu . Rev. Genet. 24 : 215 - 248 . (10.1146/annurev.ge.24.120190.001243) / Rev. Genet. / Genetic analysis of protein export in Escherichia coli. Annu by Schatz P. J. (1990)
  16. Studier , F. W. , and B. A. Moffat . 1986 . Use of bacteriophage T7 RNA polymerase to direct selective high level expression of cloned genes . J. Mol. Biol. 189 : 113 - 130 . (10.1016/0022-2836(86)90385-2) / J. Mol. Biol. / Use of bacteriophage T7 RNA polymerase to direct selective high level expression of cloned genes by Studier F. W. (1986)
  17. Tabor , S. , and C. Richardson . 1985 . A bacteriophage T7 RNA polymerase/ promoter system for controlled exclusive expression of specific genes . Proc. Natl. Acad. Sci. USA 82 : 1074 - 1078 . (10.1073/pnas.82.4.1074) / Proc. Natl. Acad. Sci. USA / A bacteriophage T7 RNA polymerase/ promoter system for controlled exclusive expression of specific genes by Tabor S. (1985)
  18. Tommassen , J. , A. Filloux , M. Bally , M. Murgier , and A. Lazdunski . 1992 . Protein secretion in Pseudomonas aeruginosa . FEMS Microbiol. Rev. 103 : 73 - 90 . (10.1111/j.1574-6968.1992.tb05824.x) / FEMS Microbiol. Rev. / Protein secretion in Pseudomonas aeruginosa by Tommassen J. (1992)
  19. von Heijne , G. 1992 . Membrane protein structure prediction. Hydrophobicity analysis and positive-inside rule . J. Mol. Biol. 225 : 487 - 494 . (10.1016/0022-2836(92)90934-C) / J. Mol. Biol. / Membrane protein structure prediction. Hydrophobicity analysis and positive-inside rule by von Heijne G. (1992)
  20. Walker , J. E. , M. Saraste , M. J. Runswick , and N. J. Gay . 1982 . Distantly related sequences in the ~- and ~-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold . EMBO J. 8 : 945 - 951 . (10.1002/j.1460-2075.1982.tb01276.x) / EMBO J. / Distantly related sequences in the ~- and ~-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold by Walker J. E. (1982)
  21. Whitley , P. , T. Zander , M. Ehrmann , M. Haardt , E. Bremer , and G. von Heijne . 1994 . Sec-independent translocation of a 100-residue periplasmic N-terminal tail in the E. coli inner membrane protein proW . EMBO J. 13 : 4653 - 4661 . (10.1002/j.1460-2075.1994.tb06788.x) / EMBO J. / Sec-independent translocation of a 100-residue periplasmic N-terminal tail in the E. coli inner membrane protein proW by Whitley P. (1994)
Dates
Type When
Created 8 years, 9 months ago (Nov. 14, 2016, 5:39 a.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 1:56 p.m.)
Indexed 3 days, 23 hours ago (Aug. 26, 2025, 2:52 a.m.)
Issued 29 years, 1 month ago (July 1, 1996)
Published 29 years, 1 month ago (July 1, 1996)
Published Print 29 years, 1 month ago (July 1, 1996)
Funders 0

None

@article{Bleves_1996, title={Membrane topology of three Xcp proteins involved in exoprotein transport by Pseudomonas aeruginosa}, volume={178}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.178.14.4297-4300.1996}, DOI={10.1128/jb.178.14.4297-4300.1996}, number={14}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Bleves, S and Lazdunski, A and Filloux, A}, year={1996}, month=jul, pages={4297–4300} }