Homologous pairs of regulatory proteins control activity of Bacillus subtilis transcription factor sigma(b) in response to environmental stress
10.1128/jb.178.13.3846-3853.1996
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

In Bacillus subtilis, activity of the general stress transcription factor sigma B is controlled posttranslationally by a regulatory network that transmits signals of environmental and metabolic stress. These signals include heat, ethanol, or osmotic challenge, or a sharp decrease in cellular energy levels, and all ultimately control sigma B activity by influencing the binding decision of the RsbW anti-sigma factor. In the absence of stress, RsbW binds to sigma B and prevents its association with RNA polymerase core enzyme. However, following stress, RsbW binds instead to the RsbV anti-anti-sigma factor, thereby releasing sigma B to direct transcription of its target genes. These two principal regulators of sigmaB activity are encoded in the eight-gene sigB operon, which has the gene order rsbR-rsbS-rsbT-rsbU-rsbV-rsbW-sig B-rsbX (where rsb stands for regulator of sigma B). Notably, the predicted rsbS product has significant amino acid identity to the RsbV anti-anti-sigma factor and the predicted rsbT product resembles the RsbW anti-sigma factor. To determine the roles of rsbS and rsbT, null or missense mutations were constructed in the chromosomal copies or each and tested for their effects on expression of a sigma B-dependent reporter fusion. On the basis of this genetic analysis, our principal conclusions are that (i) the rsbS product is a negative regulator of or" activity, (ii) the rsbT product is a positive regulator, (iii) RsbS requires RsbT for function, and (iv) the RsbS-RsbT and RsbV-RsbW pairs act hierarchically by a common mechanism in which key protein-protein interactions are controlled by phosphorylation events.

Bibliography

Kang, C. M., Brody, M. S., Akbar, S., Yang, X., & Price, C. W. (1996). Homologous pairs of regulatory proteins control activity of Bacillus subtilis transcription factor sigma(b) in response to environmental stress. Journal of Bacteriology, 178(13), 3846–3853.

Authors 5
  1. C M Kang (first)
  2. M S Brody (additional)
  3. S Akbar (additional)
  4. X Yang (additional)
  5. C W Price (additional)
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Dates
Type When
Created 8 years, 9 months ago (Nov. 29, 2016, 12:35 p.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 1:57 p.m.)
Indexed 1 month, 3 weeks ago (July 11, 2025, 6:29 a.m.)
Issued 29 years, 2 months ago (July 1, 1996)
Published 29 years, 2 months ago (July 1, 1996)
Published Print 29 years, 2 months ago (July 1, 1996)
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@article{Kang_1996, title={Homologous pairs of regulatory proteins control activity of Bacillus subtilis transcription factor sigma(b) in response to environmental stress}, volume={178}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.178.13.3846-3853.1996}, DOI={10.1128/jb.178.13.3846-3853.1996}, number={13}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Kang, C M and Brody, M S and Akbar, S and Yang, X and Price, C W}, year={1996}, month=jul, pages={3846–3853} }