DOI: 10.1128/jb.177.22.6652-6656.1995. Regulation of malate dehydrogenase (mdh) gene expression in Escherichia coli in response to oxygen, carbon, and heme availability

Regulation of malate dehydrogenase (mdh) gene expression in Escherichia coli in response to oxygen, carbon, and heme availability

10.1128/jb.177.22.6652-6656.1995

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

Malate dehydrogenase catalyzes the interconversion of malate and oxaloacetate. It participates as a member of the tricarboxylic acid cycle and the branched noncyclic pathways under aerobic and anaerobic cell growth conditions, respectively. To investigate how the mdh gene is expressed under these different conditions, an mdh-lacZ operon fusion was constructed and analyzed in vivo. The mdh-lacZ fusion was expressed about twofold higher under aerobic conditions than under anaerobic cell growth conditions on most media tested. This anaerobic response is modulated by the ArcA protein, which functions as a repressor of mdh gene expression under both aerobic and anaerobic conditions. In contrast, mutations in the fnr gene did not affect mdh gene expression. Interestingly, cells grown anaerobically with glycerol and trimethylamine N-oxide or fumarate showed higher levels of mdh expression than did cells that were grown aerobically. Depending on the type of carbon compound used for cell growth, mdh expression varied by 11-fold and 5-fold under aerobic and anaerobic conditions, respectively. While mdh transcription was shown to be inversely proportional to the cell growth rate, cellular heme limitation stimulated a fivefold increase in mdh gene expression. The mdh gene appears to be highly regulated to adapt to changing conditions of aerobic and anaerobic cell growth with various types of carbon substrates.

Bibliography

Park, S. J., Cotter, P. A., & Gunsalus, R. P. (1995). Regulation of malate dehydrogenase (mdh) gene expression in Escherichia coli in response to oxygen, carbon, and heme availability. Journal of Bacteriology, 177(22), 6652â6656.

Authors 3

S J Park (first)
P A Cotter (additional)
R P Gunsalus (additional)

References 22 Referenced 88

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Chiang , R. C. , R. Cavicchioli , and R. P. Gunsalus . 1992 . Identification and characterization of narQ, a second nitrate sensor for nitrate-dependent gene regulation in Escherichia coli . Mol. Microbiol. 6 : 1913 - 1923 . (10.1111/j.1365-2958.1992.tb01364.x) / Mol. Microbiol. / Identification and characterization of narQ, a second nitrate sensor for nitrate-dependent gene regulation in Escherichia coli by Chiang R. C. (1992)
Cotter , P. A. , and R. P. Gunsalus . 1989 . Oxygen, nitrate, and molybdenum regulation of dmsABC gene expression in Escherichia coli . J. Bacteriol. 171 : 3817 - 3823 . (10.1128/jb.171.7.3817-3823.1989) / J. Bacteriol. / Oxygen, nitrate, and molybdenum regulation of dmsABC gene expression in Escherichia coli by Cotter P. A. (1989)
Cotter , P. A. , and R. P. Gunsalus . 1992 . Contribution of the fnr and arcA gene products in coordinate regulation of the cytochrome o (cyoABCDE) and d (cydAB) oxidase genes in Escherichia coli . FEMS Microbiol. Lett. 91 : 31 - 36 . (10.1111/j.1574-6968.1992.tb05179.x) / FEMS Microbiol. Lett. / Contribution of the fnr and arcA gene products in coordinate regulation of the cytochrome o (cyoABCDE) and d (cydAB) oxidase genes in Escherichia coli by Cotter P. A. (1992)
Darie , S. , and R. P. Gunsalus . 1994 . Effect of heme and oxygen availability on hemA gene expression in Escherichia coli: role of the fnr, arcA, and himA gene products . J. Bacteriol. 176 : 5270 - 5276 . (10.1128/jb.176.17.5270-5276.1994) / J. Bacteriol. / Effect of heme and oxygen availability on hemA gene expression in Escherichia coli: role of the fnr, arcA, and himA gene products by Darie S. (1994)
Gray , C. T. , J. W. T. Wimpenny , and M. R. Mossman . 1966 . Regulation of metabolism in facultative bacteria. II. Effects of aerobiosis, anaerobiosis and nutrition on the formation of Krebs cycle enzymes in Escherichia coli . Biochim. Biophys. Acta 117 : 33 - 41 . (10.1016/0304-4165(66)90149-8) / Biochim. Biophys. Acta / Regulation of metabolism in facultative bacteria. II. Effects of aerobiosis, anaerobiosis and nutrition on the formation of Krebs cycle enzymes in Escherichia coli by Gray C. T. (1966)
Gunsalus , R. P. , and S. -J. Park . 1994 . Aerobic-anaerobic gene regulation in Escherichia coli: control by the ArcAB and Fnr regulons . Res. Microbiol. 145 : 437 - 450 . (10.1016/0923-2508(94)90092-2) / Res. Microbiol. / Aerobic-anaerobic gene regulation in Escherichia coli: control by the ArcAB and Fnr regulons by Gunsalus R. P. (1994)
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Iuchi , S. , and E. C. C. Lin . 1988 . arcA (dye), a global regulatory gene in Escherichia coli mediating repression of enzymes in aerobic pathways . Proc. Natl. Acad. Sci. USA 85 : 1888 - 1892 . (10.1073/pnas.85.6.1888) / Proc. Natl. Acad. Sci. USA / arcA (dye), a global regulatory gene in Escherichia coli mediating repression of enzymes in aerobic pathways by Iuchi S. (1988)
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Park , S. - J. , J. McCabe , J. Turna , and R. P. Gunsalus . 1994 . Regulation of the citrate synthase (gltA) gene of Escherichia coli in response to anaerobiosis and carbon supply: role of the arcA gene product . J. Bacteriol. 176 : 5086 - 5092 . (10.1128/jb.176.16.5086-5092.1994) / J. Bacteriol. / Regulation of the citrate synthase (gltA) gene of Escherichia coli in response to anaerobiosis and carbon supply: role of the arcA gene product by Park S. (1994)
Park , S. - J. , C. P. Tseng , and R. P. Gunsalus . 1994 . Regulation of the Escherichia coli succinate dehydrogenase (sdhCDAB) operon in response to anaerobiosis and medium richness: role of the ArcA and Fnr proteins . Mol. Microbiol. 15 : 473 - 482 . (10.1111/j.1365-2958.1995.tb02261.x) / Mol. Microbiol. / Regulation of the Escherichia coli succinate dehydrogenase (sdhCDAB) operon in response to anaerobiosis and medium richness: role of the ArcA and Fnr proteins by Park S. (1994)
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Dates

Type	When
Created	8 years, 9 months ago (Nov. 9, 2016, 10:42 a.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:53 p.m.)
Indexed	1 month, 1 week ago (July 23, 2025, 8:03 a.m.)
Issued	29 years, 9 months ago (Nov. 1, 1995)
Published	29 years, 9 months ago (Nov. 1, 1995)
Published Print	29 years, 9 months ago (Nov. 1, 1995)

Funders 0

None

BibTeX

@article{Park_1995, title={Regulation of malate dehydrogenase (mdh) gene expression in Escherichia coli in response to oxygen, carbon, and heme availability}, volume={177}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.177.22.6652-6656.1995}, DOI={10.1128/jb.177.22.6652-6656.1995}, number={22}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Park, S J and Cotter, P A and Gunsalus, R P}, year={1995}, month=nov, pages={6652–6656} }

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