Cloning, sequencing, and expression in Escherichia coli of the Bacillus subtilis gene for phosphatidylserine synthase
10.1128/jb.176.24.7456-7461.1994
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

The Bacillus subtilis pss gene encoding phosphatidylserine synthase was cloned by its complementation of the temperature sensitivity of an Escherichia coli pssA1 mutant. Nucleotide sequencing of the clone indicated that the pss gene encodes a polypeptide of 177 amino acid residues (deduced molecular weight of 19,613). This value agreed with the molecular weight of approximately 18,000 observed for the maxicell product. The B. subtilis phosphatidylserine synthase showed 35% amino acid sequence homology to the yeast Saccharomyces cerevisiae phosphatidylserine synthase and had a region with a high degree of local homology to the conserved segments in some phospholipid synthases and amino alcohol phosphotransferases of E. coli and S. cerevisiae, whereas no homology was found with that of the E. coli counterpart. A hydropathy analysis revealed that the B. subtilis synthase is very hydrophobic, in contrast to the hydrophilic E. coli counterpart, consisting of several strongly hydrophobic segments that would span the membrane. A manganese-dependent phosphatidylserine synthase activity, a characteristic of the B. subtilis enzyme, was found exclusively in the membrane fraction of E. coli (pssA1) cells harboring a B. subtilis pss plasmid. Overproduction of the B. subtilis synthase in E. coli cells by a lac promoter system resulted in an unusual increase of phosphatidylethanolamine (up to 93% of the total phospholipids), in contrast to gratuitous overproduction of the E. coli counterpart. This finding suggested that the unusual cytoplasmic localization of the E. coli phosphatidylserine synthase plays a role in the regulation of the phospholipid polar headgroup composition in this organism.

Bibliography

Okada, M., Matsuzaki, H., Shibuya, I., & Matsumoto, K. (1994). Cloning, sequencing, and expression in Escherichia coli of the Bacillus subtilis gene for phosphatidylserine synthase. Journal of Bacteriology, 176(24), 7456–7461.

Authors 4
  1. M Okada (first)
  2. H Matsuzaki (additional)
  3. I Shibuya (additional)
  4. K Matsumoto (additional)
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Dates
Type When
Created 8 years, 9 months ago (Nov. 7, 2016, 4:37 p.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 1:20 p.m.)
Indexed 1 month, 4 weeks ago (July 2, 2025, 3:10 p.m.)
Issued 30 years, 8 months ago (Dec. 1, 1994)
Published 30 years, 8 months ago (Dec. 1, 1994)
Published Print 30 years, 8 months ago (Dec. 1, 1994)
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@article{Okada_1994, title={Cloning, sequencing, and expression in Escherichia coli of the Bacillus subtilis gene for phosphatidylserine synthase}, volume={176}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.176.24.7456-7461.1994}, DOI={10.1128/jb.176.24.7456-7461.1994}, number={24}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Okada, M and Matsuzaki, H and Shibuya, I and Matsumoto, K}, year={1994}, month=dec, pages={7456–7461} }