DOI: 10.1128/jb.176.19.6153-6156.1994. Cloning and sequence analysis of the rpsL and rpsG genes of Mycobacterium smegmatis and characterization of mutations causing resistance to streptomycin

Cloning and sequence analysis of the rpsL and rpsG genes of Mycobacterium smegmatis and characterization of mutations causing resistance to streptomycin

10.1128/jb.176.19.6153-6156.1994

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

The Mycobacterium smegmatis rpsL and rpsG genes, encoding the ribosomal proteins S12 and S7, were cloned, and their DNA sequence was determined. The third nucleotide of the S12 termination codon overlapped the first nucleotide of the S7 translation initiation codon. A collection of 28 spontaneous streptomycin-resistant mutants of M. smegmatis were isolated. All had single-base-pair substitutions in the rpsL gene which were changed to a streptomycin-sensitive phenotype by complementation with a low-copy-number plasmid carrying the wild-type M. smegmatis rpsL gene. A total of eight different mutations were found in two specific regions of the rpsL gene. Fifty-seven percent (16 of 28) altered the Lys codon at position 43. Forty-six percent of the mutations (13 of 28) were due to a transition changing an AAG Lys codon to an AGG Arg codon, with eight changes at codon 43 and five at codon 88.

Bibliography

Kenney, T. J., & Churchward, G. (1994). Cloning and sequence analysis of the rpsL and rpsG genes of Mycobacterium smegmatis and characterization of mutations causing resistance to streptomycin. Journal of Bacteriology, 176(19), 6153â6156.

Authors 2

T J Kenney (first)
G Churchward (additional)

References 20 Referenced 31

Banerjee , A*, E. Dubnau, A. Quemard , V. Balasubramanian , K. S. Um , T. Wilson , D. Collins , G. de Usle , and W. R. Jacobs , Jr. 1994 . inhA, a gene encoding a target for isoniazid and ethionamide in M. tuberculosis . Science 263 : 227 - 230 . (10.1126/science.8284673) / Science / inhA, a gene encoding a target for isoniazid and ethionamide in M. tuberculosis by Banerjee E. (1994)
Bercovier , H. , 0. Kafri , and S. Sela . 1986 . Mycobacteria possess a surprisingly small number of ribosomal RNA genes in relation to the size of their genome . Biochem. Biophys. Res. Commun. 136 : 1136 - 1141 . (10.1016/0006-291X(86)90452-3) / Biochem. Biophys. Res. Commun. / Mycobacteria possess a surprisingly small number of ribosomal RNA genes in relation to the size of their genome by Bercovier H. (1986)
Bloom , B. R. , and C. J. L. Murray . 1992 . Tuberculosis: commentary on a reemergent killer . Science 257 : 1055 - 1064 . (10.1126/science.257.5073.1055) / Science / Tuberculosis: commentary on a reemergent killer by Bloom B. R. (1992)
Cole , S. T. , and D. B. Young . 1993 . Leprosy, tuberculosis, and the new genetics . J. Bacteriol. 175 : 1 - 6 . (10.1128/jb.175.1.1-6.1993) / J. Bacteriol. / Leprosy, tuberculosis, and the new genetics by Cole S. T. (1993)
Finken , M. , P. Kirschner , A. Meier , A. Wrede , and E. Bottger . 1993 . Molecular basis of streptomycin resistance in Mycobacterium tuberculosis: alterations of the ribosomal protein S12 gene and point mutations within a functional 16S ribosomal RNA pseudoknot . Mol. Microbiol. 9 : 1239 - 1246 . (10.1111/j.1365-2958.1993.tb01253.x) / Mol. Microbiol. / Molecular basis of streptomycin resistance in Mycobacterium tuberculosis: alterations of the ribosomal protein S12 gene and point mutations within a functional 16S ribosomal RNA pseudoknot by Finken M. (1993)
Funatsu , G. , M. Yaguchi , and B. Wittmann-Liebold . 1977 . Primary structure of protein S12 from the small Escherichia coli ribosomal subunit . FEBS Lett. 73 : 12 - 17 . (10.1016/0014-5793(77)80004-5) / FEBS Lett. / Primary structure of protein S12 from the small Escherichia coli ribosomal subunit by Funatsu G. (1977)
Galill , S. , H. Fromm , D. Aviv , M. Edelman , and E. Galun . 1989 . Ribosomal protein S12 as a site for streptomycin resistance in Nicotiana chloroplasts . Mol. Gen. Genet. 218 : 289 - 292 . (10.1007/BF00331280) / Mol. Gen. Genet. / Ribosomal protein S12 as a site for streptomycin resistance in Nicotiana chloroplasts by Galill S. (1989)
Honor 6, N. , and S. T. Cole . 1994 . Streptomycin resistance in mycobacteria . Antimicrob. Agents Chemother. 38 : 238 - 242 . (10.1128/AAC.38.2.238) / Antimicrob. Agents Chemother. / Streptomycin resistance in mycobacteria by Honor N. (1994)
Husson , R. N. , B. E. James , and R. A. Young . 1990 . Gene replacement and expression of foreign DNA in mycobacteria . J. Bacteriol. 172 : 519 - 524 . (10.1128/jb.172.2.519-524.1990) / J. Bacteriol. / Gene replacement and expression of foreign DNA in mycobacteria by Husson R. N. (1990)
Lederberg , J. 1951 . Streptomycin resistance: a genetically recessive mutation . J. Bacteriol. 61 : 549 - 551 . (10.1128/jb.61.5.549-550.1951) / J. Bacteriol. / Streptomycin resistance: a genetically recessive mutation by Lederberg J. (1951)
Mandell G. L and M. A. Sande. 1990. Antimicrobial agents: drugs used in the chemotherapy of tuberculosis and leprosy p. 1146-1164. In L. S. Goodman A. Gilman T. W. Rall A. S. Nies and P. Taylor (ed.) Goodman & Gilman's the pharmacological basis of therapeutics 8th ed. Pergamon Press New York.
Meier , A. , P. Kirschner , F. -C. Bange , U. Vogel , and E. C. Bettger . 1994 . Genetic alterations in streptomycin-resistant Mycobacterium tuberculosis: mapping of mutations conferring resistance . Antimicrob. Agents Chemother. 38 : 228 - 233 . (10.1128/AAC.38.2.228) / Antimicrob. Agents Chemother. / Genetic alterations in streptomycin-resistant Mycobacterium tuberculosis: mapping of mutations conferring resistance by Meier A. (1994)
Rieder , H. L. , G. M. Cauthen , G. W. Comstock , and D. E. Snider . 1989 . The epidemiology of tuberculosis in the United States . Epidemiol. Rev. 11 : 79 - 98 . (10.1093/oxfordjournals.epirev.a036046) / Epidemiol. Rev. / The epidemiology of tuberculosis in the United States by Rieder H. L. (1989)
Saiki , R. K. , D. H. Gelfand , S. Stoffel , S. J. Schard R Higuchi , G. T. Horn , K. B. Mullis , and H. A. Erlich . 1988 . Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase . Science 239 : 487 - 491 . (10.1126/science.239.4839.487) / Science / Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase by Saiki R. K. (1988)
Sambrook J. E. F. Fritsch and T. Maniatis. 1989. Molecular cloning: a laboratory manual 2nd ed. Cold Spring Harbor Laboratory Press Cold Spring Harbor N.Y.
Snapper , S. B. , R. E. Melton , S. Mustafa , T. Kieser , and W. R. Jacobs . 1990 . Isolation and characterization of efficient plasmid transformation mutants of Mycobacterium smegmatis . Mol. Microbiol. 4 : 1911 - 1919 . (10.1111/j.1365-2958.1990.tb02040.x) / Mol. Microbiol. / Isolation and characterization of efficient plasmid transformation mutants of Mycobacterium smegmatis by Snapper S. B. (1990)
Suzuki , Y. , K. Yoshinaga , Y. Ono , A. Nagata , and T. Yamada . 1987 . Organization of rRNA genes in Mycobacterium bovis BCG . J. Bacteriol. 169 : 839 - 843 . (10.1128/jb.169.2.839-843.1987) / J. Bacteriol. / Organization of rRNA genes in Mycobacterium bovis BCG by Suzuki Y. (1987)
Telenti , A. P. , P. Imboden , F. Marchesi , D. Lowrie , S. Cole , L. Matter , K. Schopfer , and T. Bodmer . 1993 . Detection of rifampicin-resistance mutations in Mycobacterium tuberculosis . Lancet 341 : 647 - 650 . (10.1016/0140-6736(93)90417-F) / Lancet / Detection of rifampicin-resistance mutations in Mycobacterium tuberculosis by Telenti A. P. (1993)
Wittmann H. G. J. A. Littlechild and B. Wittmann-Liebold. 1980. Structure of ribosomal proteins p. 51-88. In G. Chambliss G. R. Craven J. Davies K. Davis L. Kahan and M. Nomura (ed.) Ribosomes: structure function and genetics. University Park Press Baltimore.
Yanisch-Perron , C. , J. Vieira , and J. Messing . 1985 . Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl 8 and pUC19 vectors . Gene 33 : 103 - 119 . (10.1016/0378-1119(85)90120-9) / Gene / Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl 8 and pUC19 vectors by Yanisch-Perron C. (1985)

Dates

Type	When
Created	8 years, 9 months ago (Nov. 8, 2016, 11:12 a.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:54 p.m.)
Indexed	2 months, 4 weeks ago (June 4, 2025, 6:06 a.m.)
Issued	30 years, 11 months ago (Oct. 1, 1994)
Published	30 years, 11 months ago (Oct. 1, 1994)
Published Print	30 years, 11 months ago (Oct. 1, 1994)

Funders 0

None

BibTeX

@article{Kenney_1994, title={Cloning and sequence analysis of the rpsL and rpsG genes of Mycobacterium smegmatis and characterization of mutations causing resistance to streptomycin}, volume={176}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.176.19.6153-6156.1994}, DOI={10.1128/jb.176.19.6153-6156.1994}, number={19}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Kenney, T J and Churchward, G}, year={1994}, month=oct, pages={6153–6156} }

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    "1098-5530"
  ],
  "subject": [],
  "container-title-short": "J Bacteriol",
  "published": {
    "date-parts": [
      [
        1994,
        10
      ]
    ]
  },
  "assertion": [
    {
      "value": "2021-02-02",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}