MapA, an iron-regulated, cytoplasmic membrane protein in the cyanobacterium Synechococcus sp. strain PCC7942
10.1128/jb.176.16.4906-4913.1994
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

Growth of Synechococcus sp. strain PCC 7942 in iron-deficient media leads to the accumulation of an approximately 34-kDa protein. The gene encoding this protein, mapA (membrane-associated protein A), has been cloned and sequenced (GenBank accession number, L01621). The mapA transcript is not detectable in normally grown cultures but is stably accumulated by cells grown in iron-deficient media. However, the promoter sequence for this gene does not resemble other bacterial iron-regulated promoters described to date. The carboxyl-terminal region of the derived amino acid sequence of MapA resembles bacterial proteins involved in iron acquisition, whereas the amino-terminal end of MapA has a high degree of amino acid identity with the abundant, chloroplast envelope protein E37. An approach employing improved cellular fractionation techniques as well as electron microscopy and immunocytochemistry was essential in localizing MapA protein to the cytoplasmic membrane of Synechococcus sp. strain PCC 7942. When these cells were grown under iron-deficient conditions, a significant fraction of MapA could also be localized to the thylakoid membranes.

Bibliography

Webb, R., Troyan, T., Sherman, D., & Sherman, L. A. (1994). MapA, an iron-regulated, cytoplasmic membrane protein in the cyanobacterium Synechococcus sp. strain PCC7942. Journal of Bacteriology, 176(16), 4906–4913.

Authors 4
  1. R Webb (first)
  2. T Troyan (additional)
  3. D Sherman (additional)
  4. L A Sherman (additional)
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Dates
Type When
Created 8 years, 9 months ago (Nov. 14, 2016, 5:37 a.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 1:18 p.m.)
Indexed 1 month ago (Aug. 6, 2025, 9:24 a.m.)
Issued 31 years, 1 month ago (Aug. 1, 1994)
Published 31 years, 1 month ago (Aug. 1, 1994)
Published Print 31 years, 1 month ago (Aug. 1, 1994)
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@article{Webb_1994, title={MapA, an iron-regulated, cytoplasmic membrane protein in the cyanobacterium Synechococcus sp. strain PCC7942}, volume={176}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.176.16.4906-4913.1994}, DOI={10.1128/jb.176.16.4906-4913.1994}, number={16}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Webb, R and Troyan, T and Sherman, D and Sherman, L A}, year={1994}, month=aug, pages={4906–4913} }