DOI: 10.1128/jb.176.13.3832-3838.1994. Transcriptional organization of the trans-regulatory locus which controls exoenzyme S synthesis in Pseudomonas aeruginosa

Transcriptional organization of the trans-regulatory locus which controls exoenzyme S synthesis in Pseudomonas aeruginosa

10.1128/jb.176.13.3832-3838.1994

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

The transcriptional organization of the exoenzyme S trans-regulatory locus was studied by using promoter fusion and transcriptional start site mapping analyses. The 5' regions flanking open reading frames encoding ExsC, ExsB, ExsA, and ExsD were cloned in both orientations into the promoter vector pQF26, which contains the chloramphenicol acetyltransferase reporter gene (cat). CAT activity from each promoter fusion transformed into Pseudomonas aeruginosa and Escherichia coli was measured. The trans-regulatory locus promoters demonstrated low to undetectable CAT activity in E. coli regardless of the orientation of the DNA fragment relative to the reporter gene. In P. aeruginosa two of the promoter clones containing DNA located 5' of exsC (pC) and exsD (pD) demonstrated significant CAT activity. Transcriptional initiation from pC and pD was dependent on the orientation of the DNA fragment, the inclusion of a chelator in the growth medium, and the presence of a functional exsA gene. Transcriptional start sites were mapped for the pC and pD promoter regions by using total RNA isolated from P. aeruginosa strains grown in medium including a chelator. Our data are consistent with an operon model for the transcriptional organization of the exoenzyme S trans-regulatory locus. In addition, ExsA appears to be involved in controlling transcriptional initiation from both the trans-regulatory locus and a region located immediately downstream of the exsA gene.

Bibliography

Yahr, T. L., & Frank, D. W. (1994). Transcriptional organization of the trans-regulatory locus which controls exoenzyme S synthesis in Pseudomonas aeruginosa. Journal of Bacteriology, 176(13), 3832â3838.

Authors 2

T L Yahr (first)
D W Frank (additional)

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Dates

Type	When
Created	8 years, 9 months ago (Nov. 7, 2016, 4:37 p.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:54 p.m.)
Indexed	1 month ago (July 27, 2025, 3:26 a.m.)
Issued	31 years, 1 month ago (July 1, 1994)
Published	31 years, 1 month ago (July 1, 1994)
Published Print	31 years, 1 month ago (July 1, 1994)

Funders 0

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BibTeX

@article{Yahr_1994, title={Transcriptional organization of the trans-regulatory locus which controls exoenzyme S synthesis in Pseudomonas aeruginosa}, volume={176}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.176.13.3832-3838.1994}, DOI={10.1128/jb.176.13.3832-3838.1994}, number={13}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Yahr, T L and Frank, D W}, year={1994}, month=jul, pages={3832–3838} }

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