DOI: 10.1128/jb.176.1.260-264.1994. Vancomycin-resistant Leuconostoc mesenteroides and Lactobacillus casei synthesize cytoplasmic peptidoglycan precursors that terminate in lactate

Vancomycin-resistant Leuconostoc mesenteroides and Lactobacillus casei synthesize cytoplasmic peptidoglycan precursors that terminate in lactate

10.1128/jb.176.1.260-264.1994

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

The emergence of acquired high-level resistance among Enterococcus species has renewed interest in mechanisms of resistance to glycopeptide antibiotics in gram-positive bacteria. In Enterococcus faecalis and Enterococcus faecium, resistance is encoded by the van gene cluster and is due to the production of a peptidoglycan precursor terminating in D-alanyl-D-lactate, to which vancomycin does not bind. Most Leuconostoc and many Lactobacillus species are intrinsically resistant to high levels of glycopeptide antibiotics, but the mechanism of resistance has not been elucidated. To determine whether the mechanisms of resistance are similar in intrinsically resistant bacteria, cytoplasmic peptidoglycan precursors were isolated from Leuconostoc mesenteroides and Lactobacillus casei and analyzed by mass spectrometry, revealing structures consistent with UDP-N-acetylmuramyl-L-Ala-D-Glu-L-Lys-(L-Ala)-D-Ala-D-lactate and UDP-N-acetylmuramyl-L-Ala-D-Glu-L-Lys-D-Ala-D-lactate, respectively.

Bibliography

Handwerger, S., Pucci, M. J., Volk, K. J., Liu, J., & Lee, M. S. (1994). Vancomycin-resistant Leuconostoc mesenteroides and Lactobacillus casei synthesize cytoplasmic peptidoglycan precursors that terminate in lactate. Journal of Bacteriology, 176(1), 260â264.

Authors 5

S Handwerger (first)
M J Pucci (additional)
K J Volk (additional)
J Liu (additional)
M S Lee (additional)

References 23 Referenced 132

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Dates

Type	When
Created	8 years, 10 months ago (Nov. 7, 2016, 2:17 p.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:54 p.m.)
Indexed	3 months, 2 weeks ago (May 18, 2025, 10:25 a.m.)
Issued	31 years, 8 months ago (Jan. 1, 1994)
Published	31 years, 8 months ago (Jan. 1, 1994)
Published Print	31 years, 8 months ago (Jan. 1, 1994)

Funders 0

None

BibTeX

@article{Handwerger_1994, title={Vancomycin-resistant Leuconostoc mesenteroides and Lactobacillus casei synthesize cytoplasmic peptidoglycan precursors that terminate in lactate}, volume={176}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.176.1.260-264.1994}, DOI={10.1128/jb.176.1.260-264.1994}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Handwerger, S and Pucci, M J and Volk, K J and Liu, J and Lee, M S}, year={1994}, month=jan, pages={260–264} }

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