Eight genes in region 5 that form an operon are essential for invasion of epithelial cells by Shigella flexneri 2a
10.1128/jb.175.8.2334-2346.1993
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

The 7-kb region 5 on the large 230-kb plasmid pMYSH6000 in Shigella flexneri 2a YSH6000 is one of the virulence-associated DNA segments required for the invasion of epithelial cells (C. Sasakawa, K. Kamata, T. Sakai, S. Makino, M. Yamada, N. Okada, and M. Yoshikawa, J. Bacteriol. 170:2480-2484, 1988). To elucidate the functional organization of region 5 and to determine the virulence-associated genes encoded by region 5, we performed insertion and deletion mutagenesis, DNA subcloning, and complete nucleotide sequencing of region 5 and found that region 5 contained 11 open reading frames (ORFs) named ORF-1 through ORF-11 which could be translated into proteins with molecular masses of 15.1, 47.5, 13.2, 33.0, 33.4, 24.2, 9.4, 28.5, 39.9, 9.1, and 10.4 kDa, respectively. Complementation tests of the 14 Tn5-induced noninvasive mutants of region 5 with the above plasmid constructs have indicated that region 5 consists of an operon and that ORF-2 through ORF-9, but not ORF-1, ORF-10, and ORF-11, are essential for invasion, and 7 of 8 ORFs (ORF-2 and ORF-4 through ORF-9) and presumably the remaining ORF (ORF-3) are required for secretion of the Ipa proteins. The transcriptional organization, as determined by a promoter-proving vector, S1 nuclease protection, and primer extension RNA sequencing analysis revealed that region 5 is transcribed from a promoter located 47 bp upstream of the 5' end of ORF-2 for the 47.5-kDa protein and that the promoter activity identified was regulated by the virB gene, the transcriptional activator on the 230-kb plasmid.

Bibliography

Sasakawa, C., Komatsu, K., Tobe, T., Suzuki, T., & Yoshikawa, M. (1993). Eight genes in region 5 that form an operon are essential for invasion of epithelial cells by Shigella flexneri 2a. Journal of Bacteriology, 175(8), 2334–2346.

Authors 5
  1. C Sasakawa (first)
  2. K Komatsu (additional)
  3. T Tobe (additional)
  4. T Suzuki (additional)
  5. M Yoshikawa (additional)
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Dates
Type When
Created 8 years, 9 months ago (Nov. 7, 2016, 9:48 a.m.)
Deposited 4 years ago (July 29, 2021, 1:53 p.m.)
Indexed 1 year, 7 months ago (Jan. 18, 2024, 12:28 a.m.)
Issued 32 years, 4 months ago (April 1, 1993)
Published 32 years, 4 months ago (April 1, 1993)
Published Print 32 years, 4 months ago (April 1, 1993)
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@article{Sasakawa_1993, title={Eight genes in region 5 that form an operon are essential for invasion of epithelial cells by Shigella flexneri 2a}, volume={175}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.175.8.2334-2346.1993}, DOI={10.1128/jb.175.8.2334-2346.1993}, number={8}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Sasakawa, C and Komatsu, K and Tobe, T and Suzuki, T and Yoshikawa, M}, year={1993}, month=apr, pages={2334–2346} }