High-level transcription of the major Bacillus subtilis autolysin operon depends on expression of the sigma D gene and is affected by a sin (flaD) mutation
10.1128/jb.175.3.795-801.1993
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

Transcription of the major Bacillus subtilis autolysin gene (cwlB) was investigated. Deletion of the region upstream of the gene cluster lppX-cwbA-cwlB led to a loss of promoter activity. Primer extension analysis suggested that the cwlB operon is transcribed by E sigma D and E sigma A, the former transcripts being predominants at the exponential growth phase. Expression of the lppX-lacZ fusion gene was reduced by about 90% in a sigD-null mutant. A sin (flaD1) mutation caused a severe defect in transcription of the lppX-cwbA-cwlB operon. The sin (flaD1) mutation also reduced expression of a sigD-lacZ fusion gene constructed in the B. subtilis chromosome. Since the sigD-null mutant exhibits motility and autolysin deficiencies and filamentation, similar phenotypes in the sin (flaD1) mutant may be caused by reduction in expression of the sigma D protein.

Bibliography

Kuroda, A., & Sekiguchi, J. (1993). High-level transcription of the major Bacillus subtilis autolysin operon depends on expression of the sigma D gene and is affected by a sin (flaD) mutation. Journal of Bacteriology, 175(3), 795–801.

Authors 2
  1. A Kuroda (first)
  2. J Sekiguchi (additional)
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Dates
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Created 8 years, 9 months ago (Nov. 7, 2016, 10:32 a.m.)
Deposited 4 years ago (July 29, 2021, 1:53 p.m.)
Indexed 1 year, 9 months ago (Oct. 29, 2023, 1:45 p.m.)
Issued 32 years, 6 months ago (Feb. 1, 1993)
Published 32 years, 6 months ago (Feb. 1, 1993)
Published Print 32 years, 6 months ago (Feb. 1, 1993)
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@article{Kuroda_1993, title={High-level transcription of the major Bacillus subtilis autolysin operon depends on expression of the sigma D gene and is affected by a sin (flaD) mutation}, volume={175}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.175.3.795-801.1993}, DOI={10.1128/jb.175.3.795-801.1993}, number={3}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Kuroda, A and Sekiguchi, J}, year={1993}, month=feb, pages={795–801} }