DOI: 10.1128/jb.175.21.7006-7015.1993. Transcription from two promoters and autoregulation contribute to the control of expression of the Salmonella typhimurium flagellar regulatory gene flgM

Transcription from two promoters and autoregulation contribute to the control of expression of the Salmonella typhimurium flagellar regulatory gene flgM

10.1128/jb.175.21.7006-7015.1993

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

The flgM gene product has been shown to be a negative regulator of flagellin transcription in Salmonella typhimurium (K. L. Gillen and K. T. Hughes, J. Bacteriol. 173:2301-2310, 6453-6459, 1991; K. Ohnishi, K. Kutsukake, H. Suzuki, and T. Iino, Mol. Microbiol. 6:3149-3157, 1992). Mud-lac fusions to the flgM gene were isolated and used to characterize the regulation of flgM gene expression. Transcription of the flgM gene was decreased more than 30-fold in strains with the flagellar master regulatory genes, flhC and flhD, deleted. A class 2 flagellar defect caused a slight increase of flgM gene transcription unless a wild-type copy of the flgM gene was present, in which case transcription was decreased threefold. A deletion in the gene for the alternative sigma factor sigma 28 (FliA) caused a fourfold decrease in flgM expression. Insertional inactivation of a gene upstream of the flgM gene (flgA) in a fliA mutant strain caused transcription of the flgM gene to be decreased to a basal level. Northern (RNA) blot analysis confirmed the presence of two transcripts through the flgM gene, one which initiates upstream of the flgM gene and a second which initiates upstream of the flgA gene.

Bibliography

Gillen, K. L., & Hughes, K. T. (1993). Transcription from two promoters and autoregulation contribute to the control of expression of the Salmonella typhimurium flagellar regulatory gene flgM. Journal of Bacteriology, 175(21), 7006â7015.

Authors 2

K L Gillen (first)
K T Hughes (additional)

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Dates

Type	When
Created	8 years, 9 months ago (Nov. 14, 2016, 5:37 a.m.)
Deposited	4 years ago (July 29, 2021, 1:52 p.m.)
Indexed	1 year, 6 months ago (Feb. 3, 2024, 1:24 p.m.)
Issued	31 years, 9 months ago (Nov. 1, 1993)
Published	31 years, 9 months ago (Nov. 1, 1993)
Published Print	31 years, 9 months ago (Nov. 1, 1993)

Funders 0

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BibTeX

@article{Gillen_1993, title={Transcription from two promoters and autoregulation contribute to the control of expression of the Salmonella typhimurium flagellar regulatory gene flgM}, volume={175}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.175.21.7006-7015.1993}, DOI={10.1128/jb.175.21.7006-7015.1993}, number={21}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Gillen, K L and Hughes, K T}, year={1993}, month=nov, pages={7006–7015} }

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