Membrane topology model of Escherichia coli alpha-ketoglutarate permease by phoA fusion analysis
10.1128/jb.175.2.565-567.1993
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

Escherichia coli alpha-ketoglutarate permease (KgtP) is a 432-amino-acid protein that symports alpha-ketoglutarate and protons. KgtP was predicted to contain 12 membrane-spanning domains on the basis of a calculated hydropathy profile. The membrane topology model of KgtP was analyzed by using kgtP-phoA gene fusions and measuring alkaline phosphatase activities in cells expressing the chimeric proteins. Comparisons of the phosphatase activity levels and the locations of the KgtP-PhoA junctions are consistent with the predicted membrane topology model of KgtP.

Bibliography

Seol, W., & Shatkin, A. J. (1993). Membrane topology model of Escherichia coli alpha-ketoglutarate permease by phoA fusion analysis. Journal of Bacteriology, 175(2), 565–567.

Authors 2
  1. W Seol (first)
  2. A J Shatkin (additional)
References 20 Referenced 26
  1. Calamia , J. , and C. Manoil . 1990 . lac permease of Escherichia coli: topology and sequence elements promoting membrane insertion . Proc. Natl. Acad. Sci. USA 87 : 4937 - 4941 . (10.1073/pnas.87.13.4937) / Proc. Natl. Acad. Sci. USA / lac permease of Escherichia coli: topology and sequence elements promoting membrane insertion by Calamia J. (1990)
  2. Devereux , J. , P. Haeberli , and 0. Smithies . 1984 . A comprehensive set of sequence analysis programs for the VAX . Nucleic Acids Res. 12 : 387 - 395 . (10.1093/nar/12.1Part1.387) / Nucleic Acids Res. / A comprehensive set of sequence analysis programs for the VAX by Devereux J. (1984)
  3. Gutierrez , C. , J. Barondess , C. Manoil , and J. Beckwith . 1987 . The transposon TnphoA to detect genes for cell envelope proteins subject to a common regulatory stimulus . J. Mol. Biol. 195 : 289 - 297 . (10.1016/0022-2836(87)90650-4) / J. Mol. Biol. / The transposon TnphoA to detect genes for cell envelope proteins subject to a common regulatory stimulus by Gutierrez C. (1987)
  4. Harlow E. and D. Lane. 1988. Antibodies: a laboratory manual p. 423-470. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.
  5. Henderson , P. J. F. 1991 . Sugar transport proteins . Curr. Opin. Struct. Biol. 1 : 590 - 601 . (10.1016/S0959-440X(05)80082-X) / Curr. Opin. Struct. Biol. / Sugar transport proteins by Henderson P. J. F. (1991)
  6. Henderson , P. J. F. , and M. C. Maiden . 1990 . Homologous sugar transport proteins in Escherichia coli and their relatives in both prokaryotes and eukaryotes . Philos. Trans. R. Soc. Lond. B 326 : 391 - 410 . (10.1098/rstb.1990.0020) / Philos. Trans. R. Soc. Lond. B / Homologous sugar transport proteins in Escherichia coli and their relatives in both prokaryotes and eukaryotes by Henderson P. J. F. (1990)
  7. Hoffman , C. S. , and A. Wright . 1985 . Fusions of secreted proteins to alkaline phosphatase: an approach for studying protein secretion . Proc. Natl. Acad. Sci. USA 82 : 5107 - 5111 . (10.1073/pnas.82.15.5107) / Proc. Natl. Acad. Sci. USA / Fusions of secreted proteins to alkaline phosphatase: an approach for studying protein secretion by Hoffman C. S. (1985)
  8. Kaback , H. R. , E. Bibi , and P. D. Roepe . 1990 . 1-Galactoside transport in E. coli: a functional dissection of lac permease . Trends Biochem. Sci. 15 : 309 - 314 . (10.1016/0968-0004(90)90020-C) / Trends Biochem. Sci. / 1-Galactoside transport in E. coli: a functional dissection of lac permease by Kaback H. R. (1990)
  9. Laemmli , U. K. 1970 . Cleavage of structural proteins during the assembly of the head of bacteriophage T4 . Nature (London) 227 : 680 - 685 . (10.1038/227680a0) / Nature (London) / Cleavage of structural proteins during the assembly of the head of bacteriophage T4 by Laemmli U. K. (1970)
  10. Manoil , C. , and J. Beckwith . 1985 . TnphoA: a transposon probe for protein export signals . Proc. Natl. Acad. Sci. USA 82 : 8129 - 8133 . (10.1073/pnas.82.23.8129) / Proc. Natl. Acad. Sci. USA / TnphoA: a transposon probe for protein export signals by Manoil C. (1985)
  11. Sambrook J. E. F. Fritsch and T. Maniatis. 1989. Molecular cloning: a laboratory manual 2nd ed. p. 13.34-13.41 15.14-15.26. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.
  12. Sanger , F. , S. Nicklen , and A. R. Coulson . 1977 . DNA sequencing with chain-terminating inhibitors . J. Mol. Biol. 143 : 161 - 178 . / J. Mol. Biol. / DNA sequencing with chain-terminating inhibitors by Sanger F. (1977)
  13. Seol W. 1992. Ph.D. thesis. Rutgers University New Brunswick N.J.
  14. Seol , W. , and A. J. Shatkin . 1990 . A new gene located between pss and rnG on the Escherichia coli chromosome . J. Bacteriol. 172 : 4745 . / J. Bacteriol. / A new gene located between pss and rnG on the Escherichia coli chromosome by Seol W. (1990)
  15. Seol , W. , and A. J. Shatkin . 1991 . Eschenchia coli kgtp encodes an a-ketoglutarate transporter . Proc. Natl. Acad. Sci. USA 88 : 3802 - 3806 . (10.1073/pnas.88.9.3802) / Proc. Natl. Acad. Sci. USA / Eschenchia coli kgtp encodes an a-ketoglutarate transporter by Seol W. (1991)
  16. Seol , W. , and A. J. Shatkin . 1992 . E. coli a-ketoglutarate permease (KgtP) is a constitutively expressed proton symporter . J. Biol. Chem. 267 : 6409 - 6413 . (10.1016/S0021-9258(18)42710-X) / J. Biol. Chem. / E. coli a-ketoglutarate permease (KgtP) is a constitutively expressed proton symporter by Seol W. (1992)
  17. Seol , W. , and A. J. Shatkin . 1992 . Characterization of sitedirected mutants of cx-ketoglutarate permease . Biochemistry 31 : 3550 - 3554 . (10.1021/bi00128a032) / Biochemistry / Characterization of sitedirected mutants of cx-ketoglutarate permease by Seol W. (1992)
  18. Studier , F. W. , and B. A. Moffatt . 1986 . Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes . J. Mol. Biol. 189 : 113 - 130 . (10.1016/0022-2836(86)90385-2) / J. Mol. Biol. / Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes by Studier F. W. (1986)
  19. Sugiyama , J. E. , S. Mahmoodian , and G. R. Jacobson . 1991 . Membrane topology analysis of Eschenichia coli mannitol permease by using a nested-deletion method to create mtlA-phoA fusions . Proc. Natl. Acad. Sci. USA 88 : 9603 - 9607 . (10.1073/pnas.88.21.9603) / Proc. Natl. Acad. Sci. USA / Membrane topology analysis of Eschenichia coli mannitol permease by using a nested-deletion method to create mtlA-phoA fusions by Sugiyama J. E. (1991)
  20. Yun , C. , S. R. Van Doren , A. R. Crofts , and R. B. Gennis . 1991 . The use of gene fusions to examine the membrane topology of the L-subunit of the photosynthetic reaction center and of the cytochrome b subunit of the bcl complex from Rhodobacter sphaeroides . J. Biol. Chem. 266 : 10967 - 10973 . (10.1016/S0021-9258(18)99114-3) / J. Biol. Chem. / The use of gene fusions to examine the membrane topology of the L-subunit of the photosynthetic reaction center and of the cytochrome b subunit of the bcl complex from Rhodobacter sphaeroides by Yun C. (1991)
Dates
Type When
Created 8 years, 9 months ago (Nov. 7, 2016, 10:22 a.m.)
Deposited 4 years ago (July 29, 2021, 1:17 p.m.)
Indexed 1 year, 10 months ago (Oct. 24, 2023, 11:28 a.m.)
Issued 32 years, 7 months ago (Jan. 1, 1993)
Published 32 years, 7 months ago (Jan. 1, 1993)
Published Print 32 years, 7 months ago (Jan. 1, 1993)
Funders 0

None

@article{Seol_1993, title={Membrane topology model of Escherichia coli alpha-ketoglutarate permease by phoA fusion analysis}, volume={175}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.175.2.565-567.1993}, DOI={10.1128/jb.175.2.565-567.1993}, number={2}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Seol, W and Shatkin, A J}, year={1993}, month=jan, pages={565–567} }