DOI: 10.1128/jb.175.19.6126-6133.1993. Three distinct secreted aspartyl proteinases in Candida albicans

Three distinct secreted aspartyl proteinases in Candida albicans

10.1128/jb.175.19.6126-6133.1993

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

The secreted aspartyl proteinases of Candida albicans (products of the SAP genes) are thought to contribute to virulence through their effects on Candida adherence, invasion, and pathogenicity. From a single strain of C. albicans (WO-1) which expresses a phenotypic switching system, three secreted aspartyl proteinases have been identified as determined by molecular weight and N-terminal sequence. Each of the three identified proteins represents the mature form of one of three distinct proteinase isoenzymes, two of which correspond to the recently cloned SAP1 and SAP2 genes (previously referred to as CAP, PEP, or PRA). A genomic library was screened under low-stringency hybridization conditions with a polymerase chain reaction fragment from SAP1. In addition to clones of SAP1 and SAP2, a clone containing SAP3, a novel third secreted proteinase gene, was identified and sequenced. The three aspartyl proteinase isoenzymes differ in primary sequence and pI, suggesting that they may play different roles in virulence and pathogenesis. All three of these proteinases are expressed in the same strain. However, the pattern of proteinase expression is correlated with the switch phenotype of the cell. Opaque cells of strain WO-1 express Sap1 and Sap3, while white cells of the same strain express Sap2. The differential expression of three Sap proteinases may contribute to virulence in C. albicans.

Bibliography

White, T. C., Miyasaki, S. H., & Agabian, N. (1993). Three distinct secreted aspartyl proteinases in Candida albicans. Journal of Bacteriology, 175(19), 6126â6133.

Authors 3

T C White (first)
S H Miyasaki (additional)
N Agabian (additional)

References 27 Referenced 128

Anderson , J. M. , and D. R. Soll . 1987 . Unique phenotype of opaque cells in the white-opaque transition of Candida albicans . J. Bacteriol. 169 : 5579 - 5588 . (10.1128/jb.169.12.5579-5588.1987) / J. Bacteriol. / Unique phenotype of opaque cells in the white-opaque transition of Candida albicans by Anderson J. M. (1987)
Ausubel F. M. R. Brent R. E. Kingston D. D. Moore J. G. Seidman J. A. Smith and K. Strubl (ed.). 1989. Current protocols in molecular biology p. 13.11.1-13.11.4. John Wiley & Sons New York.
Blagoyevich P. G. 1979. M.S. thesis. San Francisco State University San Francisco Calif.
Cutler , J. E. 1991 . Putative virulence factors of Candida albicans. Annu . Rev. Microbiol. 45 : 187 - 218 . (10.1146/annurev.mi.45.100191.001155) / Rev. Microbiol. / Putative virulence factors of Candida albicans. Annu by Cutler J. E. (1991)
DeViragh , P. A. , D. Sanglard , G. Togni , R. Faichetto , and M. Monod . 1993 . Cloning and sequencing of two Candida parapsilosis genes encoding acid proteinases . J. Gen. Microbiol. 139 : 335 - 342 . (10.1099/00221287-139-2-335) / J. Gen. Microbiol. / Cloning and sequencing of two Candida parapsilosis genes encoding acid proteinases by DeViragh P. A. (1993)
Douglas , L. J. 1986 . Candida proteinases and candidosis . Crit. Rev. Biotechnol. 8 : 121 - 129 . (10.3109/07388558809150541) / Crit. Rev. Biotechnol. / Candida proteinases and candidosis by Douglas L. J. (1986)
Ganesan , K. , A. Banerjee , and A. Datta . 1991 . Molecular cloning of the secretory acid proteinase gene from Candida albicans and its use as a species-specific probe . Infect. Immun. 59 : 2972 - 2977 . (10.1128/iai.59.9.2972-2977.1991) / Infect. Immun. / Molecular cloning of the secretory acid proteinase gene from Candida albicans and its use as a species-specific probe by Ganesan K. (1991)
Harlow E. and D. Lane. 1988. Antibodies: a laboratory manual. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.
Hube , B. , C. J. Turver , F. C. Odds , H. Eiffert , G. J. Boulnois , H. Kochel , and R. Ruchel . 1991 . Sequence of the Candida albicans gene encoding the secretory aspartate proteinase . J. Med. Vet. Mycol. 29 : 129 - 132 . (10.1080/02681219180000221) / J. Med. Vet. Mycol. / Sequence of the Candida albicans gene encoding the secretory aspartate proteinase by Hube B. (1991)
Julius , D. , A. Blake , L. Blair , R. Kunisawa , and J. Thorner . 1984 . Isolation of the putative structural gene for the Iysinearginine-cleaving endopeptidase required for processing of yeast prepro-a-factor . Cell 37 : 1075 - 1089 . (10.1016/0092-8674(84)90442-2) / Cell / Isolation of the putative structural gene for the Iysinearginine-cleaving endopeptidase required for processing of yeast prepro-a-factor by Julius D. (1984)
Lott , T. J. , L. S. Page , P. Boiron , J. Benson , and E. Reiss . 1989 . Nucleotide sequence of the Candida albicans aspartyl proteinase gene . Nucleic Acids Res. 17 : 1779 . (10.1093/nar/17.4.1779) / Nucleic Acids Res. / Nucleotide sequence of the Candida albicans aspartyl proteinase gene by Lott T. J. (1989)
Maniatis T. E. F. Fritsch and J. Sambrook. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.
Miyasaki S. H. T. C. White and N. Agabian. Submitted for publication.
Morrison , C. J. , S. F. Hurst , S. L. Bragg , R. J. Kuykendall , H. Diaz , J. Pohl , and E. Reiss . 1993 . Heterogeneity of the purified extracellular aspartyl proteinase from Candida albicans: characterization with monoclonal antibodies and N-terminal amino acid sequence analysis . Infect. Immun. 61 : 2030 - 2036 . (10.1128/iai.61.5.2030-2036.1993) / Infect. Immun. / Heterogeneity of the purified extracellular aspartyl proteinase from Candida albicans: characterization with monoclonal antibodies and N-terminal amino acid sequence analysis by Morrison C. J. (1993)
Morrow , B. , T. Srikantha , and D. R. Soll . 1992 . Transcription of the gene for a pepsinogen, PEPI, is regulated by white-opaque switching in Candida albicans . Mol. Cell. Biol. 12 : 2997 - 3005 . / Mol. Cell. Biol. / Transcription of the gene for a pepsinogen, PEPI, is regulated by white-opaque switching in Candida albicans by Morrow B. (1992)
Odds F. C. 1988. Candida and candidosis: a review and bibliography. Baihliere Tindall Philadelphia.
Ray T. L. C. D. Payne and B. J. Morrow. 1991. Candida albicans acid proteinase: characterization and role in candidiasis. In B. M. Dunn (ed.) Structure and function of the aspartic proteinases. Plenum Press New York. (10.1007/978-1-4684-6012-4_22)
Ruchel , R. , F. De Bernardis , T. L. Ray , P. A. Sullivan , and G. T. Cole . 1992 . Candida acid proteinases . J. Med. Vet. Mycol. 30(Suppl. 1 ): 123 - 132 . (10.1080/02681219280000821) / J. Med. Vet. Mycol. / Candida acid proteinases by Ruchel R. (1992)
Slutsky , B. , M. Staebell , J. Anderson , L. Risen , M. Pfalier , and D. R. Soil . 1987 . " White-opaque transition ": a second highfrequency switching system in Candida J. Bacteriol. 169 : 189 - 197 . (10.1128/jb.169.1.189-197.1987) / J. Bacteriol. / White-opaque transition by Slutsky B. (1987)
Soll , D. R. 1992 . High-frequency switching in Candida albicans . Clin. Microbiol. Rev. 5 : 183 - 203 . (10.1128/CMR.5.2.183) / Clin. Microbiol. Rev. / High-frequency switching in Candida albicans by Soll D. R. (1992)
Staib , F. 1965 . Serum-proteins as nitrogen source for yeast-like fungi . Sabouraudia 4 : 187 - 193 . (10.1080/00362176685190421) / Sabouraudia / Serum-proteins as nitrogen source for yeast-like fungi by Staib F. (1965)
Togni , G. , D. Sanglard , R. Falchetto , and M. Monod . 1991 . Isolation and nucleotide sequence of the extracellular acid proteinase gene (ACP) from the yeast, Candida tropicalis . FEBS Lett. 286 : 181 - 185 . (10.1016/0014-5793(91)80969-A) / FEBS Lett. / Isolation and nucleotide sequence of the extracellular acid proteinase gene (ACP) from the yeast, Candida tropicalis by Togni G. (1991)
White T. C. and N. Agabian. Unpublished data.
White T. C. S. H. Miyasaki and N. Agabian. Unpublished data.
Woodward , M. P. , W. W. Young , Jr. , and R. A. Bloodgood . 1985 . Detection of monoclonal antibodies specific for carbohydrate epitopes using periodate oxidation . J. Immunol. Methods 78 : 143 - 153 . (10.1016/0022-1759(85)90337-0) / J. Immunol. Methods / Detection of monoclonal antibodies specific for carbohydrate epitopes using periodate oxidation by Woodward M. P. (1985)
Wright , R. J. , A. Carne , A. D. Hieber , I. L. Lamont , G. W. Emerson , and P. A. Sullivan . 1992 . A second gene for a secreted aspartate proteinase in Candida albicans . J. Bacteriol. 174 : 7848 - 7853 . (10.1128/jb.174.23.7848-7853.1992) / J. Bacteriol. / A second gene for a secreted aspartate proteinase in Candida albicans by Wright R. J. (1992)
Yamamoto , T. , K. Nohara , K. Uchida , and H. Yamaguchi . 1992 . Purification and characterization of secretory proteinase of Candida albicans . Microbiol. Immunol. 36 : 637 - 664 . (10.1111/j.1348-0421.1992.tb02064.x) / Microbiol. Immunol. / Purification and characterization of secretory proteinase of Candida albicans by Yamamoto T. (1992)

Dates

Type	When
Created	8 years, 9 months ago (Nov. 7, 2016, 2:43 p.m.)
Deposited	4 years ago (July 29, 2021, 1:53 p.m.)
Indexed	3 months, 1 week ago (May 12, 2025, 12:09 p.m.)
Issued	31 years, 10 months ago (Oct. 1, 1993)
Published	31 years, 10 months ago (Oct. 1, 1993)
Published Print	31 years, 10 months ago (Oct. 1, 1993)

Funders 0

None

BibTeX

@article{White_1993, title={Three distinct secreted aspartyl proteinases in Candida albicans}, volume={175}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.175.19.6126-6133.1993}, DOI={10.1128/jb.175.19.6126-6133.1993}, number={19}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={White, T C and Miyasaki, S H and Agabian, N}, year={1993}, month=oct, pages={6126–6133} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        5,
        12
      ]
    ],
    "date-time": "2025-05-12T16:09:35Z",
    "timestamp": 1747066175571
  },
  "reference-count": 27,
  "publisher": "American Society for Microbiology",
  "issue": "19",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            1993,
            10,
            1
          ]
        ],
        "date-time": "1993-10-01T00:00:00Z",
        "timestamp": 749433600000
      },
      "content-version": "tdm",
      "delay-in-days": 0,
      "URL": "https://journals.asm.org/non-commercial-tdm-license"
    }
  ],
  "content-domain": {
    "domain": [
      "journals.asm.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1993,
        10
      ]
    ]
  },
  "abstract": "<jats:p>The secreted aspartyl proteinases of Candida albicans (products of the SAP genes) are thought to contribute to virulence through their effects on Candida adherence, invasion, and pathogenicity. From a single strain of C. albicans (WO-1) which expresses a phenotypic switching system, three secreted aspartyl proteinases have been identified as determined by molecular weight and N-terminal sequence. Each of the three identified proteins represents the mature form of one of three distinct proteinase isoenzymes, two of which correspond to the recently cloned SAP1 and SAP2 genes (previously referred to as CAP, PEP, or PRA). A genomic library was screened under low-stringency hybridization conditions with a polymerase chain reaction fragment from SAP1. In addition to clones of SAP1 and SAP2, a clone containing SAP3, a novel third secreted proteinase gene, was identified and sequenced. The three aspartyl proteinase isoenzymes differ in primary sequence and pI, suggesting that they may play different roles in virulence and pathogenesis. All three of these proteinases are expressed in the same strain. However, the pattern of proteinase expression is correlated with the switch phenotype of the cell. Opaque cells of strain WO-1 express Sap1 and Sap3, while white cells of the same strain express Sap2. The differential expression of three Sap proteinases may contribute to virulence in C. albicans.</jats:p>",
  "DOI": "10.1128/jb.175.19.6126-6133.1993",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2016,
        11,
        7
      ]
    ],
    "date-time": "2016-11-07T19:43:40Z",
    "timestamp": 1478547820000
  },
  "page": "6126-6133",
  "update-policy": "http://dx.doi.org/10.1128/asmj-crossmark-policy-page",
  "source": "Crossref",
  "is-referenced-by-count": 128,
  "title": "Three distinct secreted aspartyl proteinases in Candida albicans",
  "prefix": "10.1128",
  "volume": "175",
  "author": [
    {
      "given": "T C",
      "family": "White",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Intercampus Program in Molecular Parasitology, University of California-San Francisco 94143-1204."
        }
      ]
    },
    {
      "given": "S H",
      "family": "Miyasaki",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Intercampus Program in Molecular Parasitology, University of California-San Francisco 94143-1204."
        }
      ]
    },
    {
      "given": "N",
      "family": "Agabian",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Intercampus Program in Molecular Parasitology, University of California-San Francisco 94143-1204."
        }
      ]
    }
  ],
  "member": "235",
  "reference": [
    {
      "key": "p_1",
      "doi-asserted-by": "crossref",
      "first-page": "5579",
      "DOI": "10.1128/jb.169.12.5579-5588.1987",
      "article-title": "Unique phenotype of opaque cells in the white-opaque transition of Candida albicans",
      "volume": "169",
      "author": "Anderson J. M.",
      "year": "1987",
      "unstructured": "Anderson , J. M. , and D. R. Soll . 1987 . Unique phenotype of opaque cells in the white-opaque transition of Candida albicans . J. Bacteriol. 169 : 5579 - 5588 .",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "p_2",
      "unstructured": "Ausubel F. M. R. Brent R. E. Kingston D. D. Moore J. G. Seidman J. A. Smith and K. Strubl (ed.). 1989. Current protocols in molecular biology p. 13.11.1-13.11.4. John Wiley & Sons New York."
    },
    {
      "key": "p_3",
      "unstructured": "Blagoyevich P. G. 1979. M.S. thesis. San Francisco State University San Francisco Calif."
    },
    {
      "key": "p_4",
      "doi-asserted-by": "crossref",
      "first-page": "187",
      "DOI": "10.1146/annurev.mi.45.100191.001155",
      "article-title": "Putative virulence factors of Candida albicans. Annu",
      "volume": "45",
      "author": "Cutler J. E.",
      "year": "1991",
      "unstructured": "Cutler , J. E. 1991 . Putative virulence factors of Candida albicans. Annu . Rev. Microbiol. 45 : 187 - 218 .",
      "journal-title": "Rev. Microbiol."
    },
    {
      "key": "p_5",
      "doi-asserted-by": "crossref",
      "first-page": "335",
      "DOI": "10.1099/00221287-139-2-335",
      "article-title": "Cloning and sequencing of two Candida parapsilosis genes encoding acid proteinases",
      "volume": "139",
      "author": "DeViragh P. A.",
      "year": "1993",
      "unstructured": "DeViragh , P. A. , D. Sanglard , G. Togni , R. Faichetto , and M. Monod . 1993 . Cloning and sequencing of two Candida parapsilosis genes encoding acid proteinases . J. Gen. Microbiol. 139 : 335 - 342 .",
      "journal-title": "J. Gen. Microbiol."
    },
    {
      "key": "p_6",
      "doi-asserted-by": "crossref",
      "first-page": "121",
      "DOI": "10.3109/07388558809150541",
      "article-title": "Candida proteinases and candidosis",
      "volume": "8",
      "author": "Douglas L. J.",
      "year": "1986",
      "unstructured": "Douglas , L. J. 1986 . Candida proteinases and candidosis . Crit. Rev. Biotechnol. 8 : 121 - 129 .",
      "journal-title": "Crit. Rev. Biotechnol."
    },
    {
      "key": "p_7",
      "doi-asserted-by": "crossref",
      "first-page": "2972",
      "DOI": "10.1128/iai.59.9.2972-2977.1991",
      "article-title": "Molecular cloning of the secretory acid proteinase gene from Candida albicans and its use as a species-specific probe",
      "volume": "59",
      "author": "Ganesan K.",
      "year": "1991",
      "unstructured": "Ganesan , K. , A. Banerjee , and A. Datta . 1991 . Molecular cloning of the secretory acid proteinase gene from Candida albicans and its use as a species-specific probe . Infect. Immun. 59 : 2972 - 2977 .",
      "journal-title": "Infect. Immun."
    },
    {
      "key": "p_8",
      "unstructured": "Harlow E. and D. Lane. 1988. Antibodies: a laboratory manual. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y."
    },
    {
      "key": "p_9",
      "doi-asserted-by": "crossref",
      "first-page": "129",
      "DOI": "10.1080/02681219180000221",
      "article-title": "Sequence of the Candida albicans gene encoding the secretory aspartate proteinase",
      "volume": "29",
      "author": "Hube B.",
      "year": "1991",
      "unstructured": "Hube , B. , C. J. Turver , F. C. Odds , H. Eiffert , G. J. Boulnois , H. Kochel , and R. Ruchel . 1991 . Sequence of the Candida albicans gene encoding the secretory aspartate proteinase . J. Med. Vet. Mycol. 29 : 129 - 132 .",
      "journal-title": "J. Med. Vet. Mycol."
    },
    {
      "key": "p_10",
      "doi-asserted-by": "crossref",
      "first-page": "1075",
      "DOI": "10.1016/0092-8674(84)90442-2",
      "article-title": "Isolation of the putative structural gene for the Iysinearginine-cleaving endopeptidase required for processing of yeast prepro-a-factor",
      "volume": "37",
      "author": "Julius D.",
      "year": "1984",
      "unstructured": "Julius , D. , A. Blake , L. Blair , R. Kunisawa , and J. Thorner . 1984 . Isolation of the putative structural gene for the Iysinearginine-cleaving endopeptidase required for processing of yeast prepro-a-factor . Cell 37 : 1075 - 1089 .",
      "journal-title": "Cell"
    },
    {
      "key": "p_11",
      "doi-asserted-by": "crossref",
      "first-page": "1779",
      "DOI": "10.1093/nar/17.4.1779",
      "article-title": "Nucleotide sequence of the Candida albicans aspartyl proteinase gene",
      "volume": "17",
      "author": "Lott T. J.",
      "year": "1989",
      "unstructured": "Lott , T. J. , L. S. Page , P. Boiron , J. Benson , and E. Reiss . 1989 . Nucleotide sequence of the Candida albicans aspartyl proteinase gene . Nucleic Acids Res. 17 : 1779 .",
      "journal-title": "Nucleic Acids Res."
    },
    {
      "key": "p_12",
      "unstructured": "Maniatis T. E. F. Fritsch and J. Sambrook. 1982. Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y."
    },
    {
      "key": "p_13",
      "unstructured": "Miyasaki S. H. T. C. White and N. Agabian. Submitted for publication."
    },
    {
      "key": "p_14",
      "doi-asserted-by": "crossref",
      "first-page": "2030",
      "DOI": "10.1128/iai.61.5.2030-2036.1993",
      "article-title": "Heterogeneity of the purified extracellular aspartyl proteinase from Candida albicans: characterization with monoclonal antibodies and N-terminal amino acid sequence analysis",
      "volume": "61",
      "author": "Morrison C. J.",
      "year": "1993",
      "unstructured": "Morrison , C. J. , S. F. Hurst , S. L. Bragg , R. J. Kuykendall , H. Diaz , J. Pohl , and E. Reiss . 1993 . Heterogeneity of the purified extracellular aspartyl proteinase from Candida albicans: characterization with monoclonal antibodies and N-terminal amino acid sequence analysis . Infect. Immun. 61 : 2030 - 2036 .",
      "journal-title": "Infect. Immun."
    },
    {
      "key": "p_15",
      "first-page": "2997",
      "article-title": "Transcription of the gene for a pepsinogen, PEPI, is regulated by white-opaque switching in Candida albicans",
      "volume": "12",
      "author": "Morrow B.",
      "year": "1992",
      "unstructured": "Morrow , B. , T. Srikantha , and D. R. Soll . 1992 . Transcription of the gene for a pepsinogen, PEPI, is regulated by white-opaque switching in Candida albicans . Mol. Cell. Biol. 12 : 2997 - 3005 .",
      "journal-title": "Mol. Cell. Biol."
    },
    {
      "key": "p_16",
      "unstructured": "Odds F. C. 1988. Candida and candidosis: a review and bibliography. Baihliere Tindall Philadelphia."
    },
    {
      "key": "p_17",
      "doi-asserted-by": "crossref",
      "unstructured": "Ray T. L. C. D. Payne and B. J. Morrow. 1991. Candida albicans acid proteinase: characterization and role in candidiasis. In B. M. Dunn (ed.) Structure and function of the aspartic proteinases. Plenum Press New York.",
      "DOI": "10.1007/978-1-4684-6012-4_22"
    },
    {
      "key": "p_18",
      "doi-asserted-by": "crossref",
      "first-page": "123",
      "DOI": "10.1080/02681219280000821",
      "article-title": "Candida acid proteinases",
      "volume": "30",
      "author": "Ruchel R.",
      "year": "1992",
      "unstructured": "Ruchel , R. , F. De Bernardis , T. L. Ray , P. A. Sullivan , and G. T. Cole . 1992 . Candida acid proteinases . J. Med. Vet. Mycol. 30(Suppl. 1 ): 123 - 132 .",
      "journal-title": "J. Med. Vet. Mycol."
    },
    {
      "key": "p_19",
      "doi-asserted-by": "crossref",
      "first-page": "189",
      "DOI": "10.1128/jb.169.1.189-197.1987",
      "article-title": "White-opaque transition",
      "volume": "169",
      "author": "Slutsky B.",
      "year": "1987",
      "unstructured": "Slutsky , B. , M. Staebell , J. Anderson , L. Risen , M. Pfalier , and D. R. Soil . 1987 . \" White-opaque transition \": a second highfrequency switching system in Candida J. Bacteriol. 169 : 189 - 197 .",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "p_20",
      "doi-asserted-by": "crossref",
      "first-page": "183",
      "DOI": "10.1128/CMR.5.2.183",
      "article-title": "High-frequency switching in Candida albicans",
      "volume": "5",
      "author": "Soll D. R.",
      "year": "1992",
      "unstructured": "Soll , D. R. 1992 . High-frequency switching in Candida albicans . Clin. Microbiol. Rev. 5 : 183 - 203 .",
      "journal-title": "Clin. Microbiol. Rev."
    },
    {
      "key": "p_21",
      "doi-asserted-by": "crossref",
      "first-page": "187",
      "DOI": "10.1080/00362176685190421",
      "article-title": "Serum-proteins as nitrogen source for yeast-like fungi",
      "volume": "4",
      "author": "Staib F.",
      "year": "1965",
      "unstructured": "Staib , F. 1965 . Serum-proteins as nitrogen source for yeast-like fungi . Sabouraudia 4 : 187 - 193 .",
      "journal-title": "Sabouraudia"
    },
    {
      "key": "p_22",
      "doi-asserted-by": "crossref",
      "first-page": "181",
      "DOI": "10.1016/0014-5793(91)80969-A",
      "article-title": "Isolation and nucleotide sequence of the extracellular acid proteinase gene (ACP) from the yeast, Candida tropicalis",
      "volume": "286",
      "author": "Togni G.",
      "year": "1991",
      "unstructured": "Togni , G. , D. Sanglard , R. Falchetto , and M. Monod . 1991 . Isolation and nucleotide sequence of the extracellular acid proteinase gene (ACP) from the yeast, Candida tropicalis . FEBS Lett. 286 : 181 - 185 .",
      "journal-title": "FEBS Lett."
    },
    {
      "key": "p_23",
      "unstructured": "White T. C. and N. Agabian. Unpublished data."
    },
    {
      "key": "p_24",
      "unstructured": "White T. C. S. H. Miyasaki and N. Agabian. Unpublished data."
    },
    {
      "key": "p_25",
      "doi-asserted-by": "crossref",
      "first-page": "143",
      "DOI": "10.1016/0022-1759(85)90337-0",
      "article-title": "Detection of monoclonal antibodies specific for carbohydrate epitopes using periodate oxidation",
      "volume": "78",
      "author": "Woodward M. P.",
      "year": "1985",
      "unstructured": "Woodward , M. P. , W. W. Young , Jr. , and R. A. Bloodgood . 1985 . Detection of monoclonal antibodies specific for carbohydrate epitopes using periodate oxidation . J. Immunol. Methods 78 : 143 - 153 .",
      "journal-title": "J. Immunol. Methods"
    },
    {
      "key": "p_26",
      "doi-asserted-by": "crossref",
      "first-page": "7848",
      "DOI": "10.1128/jb.174.23.7848-7853.1992",
      "article-title": "A second gene for a secreted aspartate proteinase in Candida albicans",
      "volume": "174",
      "author": "Wright R. J.",
      "year": "1992",
      "unstructured": "Wright , R. J. , A. Carne , A. D. Hieber , I. L. Lamont , G. W. Emerson , and P. A. Sullivan . 1992 . A second gene for a secreted aspartate proteinase in Candida albicans . J. Bacteriol. 174 : 7848 - 7853 .",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "p_27",
      "doi-asserted-by": "crossref",
      "first-page": "637",
      "DOI": "10.1111/j.1348-0421.1992.tb02064.x",
      "article-title": "Purification and characterization of secretory proteinase of Candida albicans",
      "volume": "36",
      "author": "Yamamoto T.",
      "year": "1992",
      "unstructured": "Yamamoto , T. , K. Nohara , K. Uchida , and H. Yamaguchi . 1992 . Purification and characterization of secretory proteinase of Candida albicans . Microbiol. Immunol. 36 : 637 - 664 .",
      "journal-title": "Microbiol. Immunol."
    }
  ],
  "container-title": "Journal of Bacteriology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://journals.asm.org/doi/pdf/10.1128/jb.175.19.6126-6133.1993",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://journals.asm.org/doi/pdf/10.1128/jb.175.19.6126-6133.1993",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        7,
        29
      ]
    ],
    "date-time": "2021-07-29T17:53:00Z",
    "timestamp": 1627581180000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://journals.asm.org/doi/10.1128/jb.175.19.6126-6133.1993"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1993,
        10
      ]
    ]
  },
  "references-count": 27,
  "journal-issue": {
    "issue": "19",
    "published-print": {
      "date-parts": [
        [
          1993,
          10
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1128/jb.175.19.6126-6133.1993"
  ],
  "URL": "http://dx.doi.org/10.1128/jb.175.19.6126-6133.1993",
  "relation": {},
  "ISSN": [
    "0021-9193",
    "1098-5530"
  ],
  "subject": [],
  "container-title-short": "J Bacteriol",
  "published": {
    "date-parts": [
      [
        1993,
        10
      ]
    ]
  },
  "assertion": [
    {
      "value": "2021-02-02",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}