DOI: 10.1128/jb.175.14.4545-4549.1993. The lonD gene is homologous to the lon gene encoding an ATP-dependent protease and is essential for the development of Myxococcus xanthus

The lonD gene is homologous to the lon gene encoding an ATP-dependent protease and is essential for the development of Myxococcus xanthus

10.1128/jb.175.14.4545-4549.1993

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

Myxococcus xanthus contains two genes (lonV and lonD) homologous to the Escherichia coli lon gene for an ATP-dependent protease. We found that the lonD gene encodes a 90-kDa protein consisting of 827 amino acid residues. The lonD gene product shows 49, 48, and 52% sequence identity to the products of the M. xanthus lonV, E. coli lon, and Bacillus brevis lon genes, respectively. When a lonD-lacZ fusion was used, lonD was expressed during both vegetative growth and development. However, while lonD-disrupted strains were able to grow normally vegetatively, the development of M. xanthus was found to be arrested at an early stage in these strains. The mutant strains were able to form neither fruiting bodies nor myxospores.

Bibliography

Tojo, N., Inouye, S., & Komano, T. (1993). The lonD gene is homologous to the lon gene encoding an ATP-dependent protease and is essential for the development of Myxococcus xanthus. Journal of Bacteriology, 175(14), 4545â4549.

Authors 3

N Tojo (first)
S Inouye (additional)
T Komano (additional)

References 20 Referenced 72

Amerik , A. Y. , V. K. Antonov , A. E. Gorbalenya , S. A. Kotova , T. V. Rotanova , and E. V. Shimbarevich . 1991 . Site-directed mutagenesis of La protease: a catalytically active serine residue . FEBS Lett. 287 : 211 - 214 . (10.1016/0014-5793(91)80053-6) / FEBS Lett. / Site-directed mutagenesis of La protease: a catalytically active serine residue by Amerik A. Y. (1991)
Amerik , A. Y. , L. G. Chistyakova , N. I. Ostroumova , A. I. Gurevich , and V. K. Antonov . 1988 . Cloning, expression and structure of the shortened, functionally active lon gene of Escherichia coli . Bioorg. Khim. 14 : 408 - 411 . / Bioorg. Khim. / Cloning, expression and structure of the shortened, functionally active lon gene of Escherichia coli by Amerik A. Y. (1988)
Casadaban , M. J. , J. Chou , and S. N. Cohen . 1980 . In vitro gene fusions that join an enzymatically active 0-galactosidase segment to amino-terminal fragments of exogenous proteins: Eschenchia coli plasmid vectors for the detection and cloning of translational initiation signals . J. Bacteriol. 143 : 971 - 980 . (10.1128/jb.143.2.971-980.1980) / J. Bacteriol. / In vitro gene fusions that join an enzymatically active 0-galactosidase segment to amino-terminal fragments of exogenous proteins: Eschenchia coli plasmid vectors for the detection and cloning of translational initiation signals by Casadaban M. J. (1980)
Chin , D. T. , S. A. Goff , T. Webster , T. Smith , and A. L. Goldberg . 1988 . Sequence of the Ion gene in Escherichia coli . J. Biol. Chem. 263 : 11718 - 11728 . / J. Biol. Chem. / Sequence of the Ion gene in Escherichia coli by Chin D. T. (1988)
Furuichi T. M. Inouye and S. Inouye. 1985. Novel one-step cloning vector with a transposable element: application to the (10.1128/jb.164.1.270-275.1985)
Gill , R. E. , and M. G. Cull . 1986 . Control of developmental gene Myxococcus xanthus genome . J. Bacteriol. 164 : 270 - 275 . expression by cell-to-cell interactions in Myxococcus xanthus. / J. Bacteriol. / Control of developmental gene Myxococcus xanthus genome by Gill R. E. (1986)
Gill , R. E. , M. Karlok , and D. Benton . 1993 . Myxococcus J . Bacteriol. 168 : 341 - 347 . xanthus encodes an ATP-dependent protease which is required for developmental gene transcription and intercellular signaling. J. Bacteriol. 175:4538-4544. / Bacteriol. / Myxococcus J by Gill R. E. (1993)
Hagen , D. C. , A. P. Bretscher , and D. Kaiser . 1978 . Synergism between morphogenetic mutants of Myxococcus xanthus . Dev. Biol. 64 : 284 - 296 . (10.1016/0012-1606(78)90079-9) / Dev. Biol. / Synergism between morphogenetic mutants of Myxococcus xanthus by Hagen D. C. (1978)
Hagen , T. J. , and L. J. Shimkets . 1990 . Nucleotide sequence and transcriptional products of the csg locus of Myxococcus xanthus . J. Bacteriol. 172 : 15 - 23 . NOTES 4549 (10.1128/jb.172.1.15-23.1990) / J. Bacteriol. / Nucleotide sequence and transcriptional products of the csg locus of Myxococcus xanthus by Hagen T. J. (1990)
Komano , T. , T. Furuichi , M. Teintze , M. Inouye , and S. Inouye . 1984 . Effects of deletion of the gene for the developmentspecific protein S on differentiation in Myxococcus xanthus . J. Bacteriol. 158 : 1195 - 1197 . (10.1128/jb.158.3.1195-1197.1984) / J. Bacteriol. / Effects of deletion of the gene for the developmentspecific protein S on differentiation in Myxococcus xanthus by Komano T. (1984)
Kroos , L. , and D. Kaiser . 1987 . Expression of many developmentally regulated genes in Myxococcus xanthus depends on a sequence of cell interactions . Genes Dev. 1 : 840 - 854 . (10.1101/gad.1.8.840) / Genes Dev. / Expression of many developmentally regulated genes in Myxococcus xanthus depends on a sequence of cell interactions by Kroos L. (1987)
Kroos , L. , A. Kuspa , and D. Kaiser . 1986 . A global analysis of developmentally regulated genes in Myxococcus xanthus . Dev. Biol. 177 : 252 - 266 . (10.1016/0012-1606(86)90368-4) / Dev. Biol. / A global analysis of developmentally regulated genes in Myxococcus xanthus by Kroos L. (1986)
LaRossa , R. , J. Kuner , D. Hagen , C. Manoil , and D. Kaiser . 1983 . Developmental cell interactions of Myxococcus xanthus: analysis of mutants . J. Bacteriol. 153 : 1394 - 1404 . (10.1128/jb.153.3.1394-1404.1983) / J. Bacteriol. / Developmental cell interactions of Myxococcus xanthus: analysis of mutants by LaRossa R. (1983)
O'Connor , K. A. , and D. R. Zusman . 1983 . Coliphage P1-mediated transduction of cloned DNA from Eschenichia coli to Myxococcus xanthus: use for complementation and recombinational analyses . J. Bacteriol. 155 : 317 - 329 . (10.1128/jb.155.1.317-329.1983) / J. Bacteriol. / Coliphage P1-mediated transduction of cloned DNA from Eschenichia coli to Myxococcus xanthus: use for complementation and recombinational analyses by O'Connor K. A. (1983)
Rosenberg E. 1984. Myxobacteria: development and cell inter (10.1007/978-1-4613-8280-5)
Sanger , F. , S. Nicklen , and A. R. Coulson . 1977 . DNA sequencactions. Springer-Verlag, New York. ing with chain-terminating inhibitors . Proc. Natl. Acad. Sci. USA 74 : 5463 - 5467 . (10.1073/pnas.74.12.5463) / Proc. Natl. Acad. Sci. USA / DNA sequencactions. Springer-Verlag, New York. ing with chain-terminating inhibitors by Sanger F. (1977)
Shimkets , L. J. , R. E. Gill , and D. Kaiser . 1983 . Developmental cell interactions in Mfyxococcus xanthus and the spoC locus . Proc. Natl. Acad. Sci. USA 80 : 1406 - 1410 . (10.1073/pnas.80.5.1406) / Proc. Natl. Acad. Sci. USA / Developmental cell interactions in Mfyxococcus xanthus and the spoC locus by Shimkets L. J. (1983)
Tojo , N. , S. Inouye , and T. Komano . 1993 . Cloning and nucleotide sequence of the Myxococcus xanthus lon gene: indispensability of lon for vegetative growth . J. Bacteriol. 175 : 2271 - 2277 . (10.1128/jb.175.8.2271-2277.1993) / J. Bacteriol. / Cloning and nucleotide sequence of the Myxococcus xanthus lon gene: indispensability of lon for vegetative growth by Tojo N. (1993)
Yanisch-Perron C. J. Vieira and J. Messing. 1985. Improved M13 phage cloning vectors and host strains: nucleotide se (10.1016/0378-1119(85)90120-9)
Ito , K. , S. Udaka , and H. Yamagata . 1992 . Cloning, character- quences of the M13mpl 8 and pUC19 vectors . Gene 33 : 103 - 119 . / Gene / Cloning, character- quences of the M13mpl 8 and pUC19 vectors by Ito K. (1992)

Dates

Type	When
Created	8 years, 9 months ago (Nov. 7, 2016, 2:58 p.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:15 p.m.)
Indexed	1 month ago (Aug. 5, 2025, 8:37 a.m.)
Issued	32 years, 2 months ago (July 1, 1993)
Published	32 years, 2 months ago (July 1, 1993)
Published Print	32 years, 2 months ago (July 1, 1993)

Funders 0

None

BibTeX

@article{Tojo_1993, title={The lonD gene is homologous to the lon gene encoding an ATP-dependent protease and is essential for the development of Myxococcus xanthus}, volume={175}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.175.14.4545-4549.1993}, DOI={10.1128/jb.175.14.4545-4549.1993}, number={14}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Tojo, N and Inouye, S and Komano, T}, year={1993}, month=jul, pages={4545–4549} }

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      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}