Cleavage, methylation, and localization of the Pseudomonas aeruginosa export proteins XcpT, -U, -V, and -W
10.1128/jb.175.14.4375-4382.1993
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

Four components of the apparatus of extracellular protein secretion of Pseudomonas aeruginosa, Xcpt, -U, -V, and -W (XcpT-W), are synthesized as precursors with short N-terminal leader peptides that share sequence similarity with the pilin subunit of this organism. A specialized leader peptidase/methylase, product of the pilD gene, has been shown to cleave the leader peptide from prepilin and to methylate the N-terminal phenylalanine of the mature pilin. Antibodies were prepared against XcpT-W and used to purify each of these proteins. Sequence analysis of XcpT-W has shown that these proteins, like mature pilin, contain N-methylphenylalanine as the N-terminal amino acid. Analysis of cellular fractions from wild-type and pilD mutant strains of P. aeruginosa showed that the precursor forms of XcpT-W are located predominantly in the bacterial inner membrane, and their localization is not altered after PilD-mediated removal of the leader sequence. These studies demonstrate that the biogenesis of the apparatus of extracellular protein secretion and that of type IV pili share a requirement for PilD. This bifunctional enzyme, acting in the inner membrane, cleaves the leader peptides from precursors of pilins and XcpT-W and subsequently methylates the amino group of the N-terminal phenylalanine of each of its substrates.

Bibliography

Nunn, D. N., & Lory, S. (1993). Cleavage, methylation, and localization of the Pseudomonas aeruginosa export proteins XcpT, -U, -V, and -W. Journal of Bacteriology, 175(14), 4375–4382.

Authors 2
  1. D N Nunn (first)
  2. S Lory (additional)
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Dates
Type When
Created 8 years, 9 months ago (Nov. 7, 2016, 2:58 p.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 1:15 p.m.)
Indexed 3 months, 2 weeks ago (May 17, 2025, 7:21 p.m.)
Issued 32 years, 2 months ago (July 1, 1993)
Published 32 years, 2 months ago (July 1, 1993)
Published Print 32 years, 2 months ago (July 1, 1993)
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@article{Nunn_1993, title={Cleavage, methylation, and localization of the Pseudomonas aeruginosa export proteins XcpT, -U, -V, and -W}, volume={175}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.175.14.4375-4382.1993}, DOI={10.1128/jb.175.14.4375-4382.1993}, number={14}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Nunn, D N and Lory, S}, year={1993}, month=jul, pages={4375–4382} }