Structures of chaperonins from an intracellular symbiont and their functional expression in Escherichia coli groE mutants
10.1128/jb.174.6.1869-1874.1992
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

An intracellular symbiont harbored by the aphid bacteriocyte, a specialized fat body cell, synthesizes in vivo substantially only one protein, symbionin, which is a member of the chaperonin-60 family of molecular chaperones. Nucleotide sequence determination of the symbionin region of the endosymbiont genome revealed that it contains the two-cistron operon sym. Just like the Escherichia coli groE operon, the sym operon was dually led by a heat shock and an ordinary promoter sequence. According to the nucleotide sequence, symbionin was 85.5% identical to GroEL of E. coli at the amino acid sequence level. SymS, another protein encoded in the sym operon, which is a member of chaperonin-10, was 79.6% identical to GroES. Complementation experiments with E. coli groE mutants showed that the chaperonin-10 and chaperonin-60 genes from the endosymbiont are expressed in E. coli and that they can function as molecular chaperones together with endogenous GroEL and GroES, respectively.

Bibliography

Ohtaka, C., Nakamura, H., & Ishikawa, H. (1992). Structures of chaperonins from an intracellular symbiont and their functional expression in Escherichia coli groE mutants. Journal of Bacteriology, 174(6), 1869–1874.

Authors 3
  1. C Ohtaka (first)
  2. H Nakamura (additional)
  3. H Ishikawa (additional)
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Dates
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Created 8 years, 9 months ago (Nov. 4, 2016, 3:52 p.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 1:49 p.m.)
Indexed 1 month ago (July 24, 2025, 7:14 a.m.)
Issued 33 years, 5 months ago (March 1, 1992)
Published 33 years, 5 months ago (March 1, 1992)
Published Print 33 years, 5 months ago (March 1, 1992)
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@article{Ohtaka_1992, title={Structures of chaperonins from an intracellular symbiont and their functional expression in Escherichia coli groE mutants}, volume={174}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.174.6.1869-1874.1992}, DOI={10.1128/jb.174.6.1869-1874.1992}, number={6}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Ohtaka, C and Nakamura, H and Ishikawa, H}, year={1992}, month=mar, pages={1869–1874} }