Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

An antiserum against Escherichia coli Dam methyltransferase has been developed in rabbits and employed to detect and quantitate the enzyme in immunoblots. A wild-type, rapidly growing E. coli cell (doubling time = 30 min) was found to contain about 130 molecules of Dam methyltransferase.

Bibliography

Boye, E., Marinus, M. G., & Løbner-Olesen, A. (1992). Quantitation of Dam methyltransferase in Escherichia coli. Journal of Bacteriology, 174(5), 1682–1685.

Authors 3
  1. E Boye (first)
  2. M G Marinus (additional)
  3. A Løbner-Olesen (additional)
References 31 Referenced 85
  1. Allman , R. , T. Schjerven , and E. Boye . 1991 . Cell cycle parameters of Escherichia coli K-12 . J. Bacteriol. 173 : 7970 - 7974 . (10.1128/JB.173.24.7970-7974.1991) / J. Bacteriol. / Cell cycle parameters of Escherichia coli K-12 by Allman R. (1991)
  2. Arraj , J. A. , and M. G. Marinus . 1983 . Phenotypic reversal in dam mutants of Escherichia coli K-12 by a recombinant plasmid containing the dam' gene . J. Bacteriol. 153 : 562 - 565 . (10.1128/JB.153.1.562-565.1983) / J. Bacteriol. / Phenotypic reversal in dam mutants of Escherichia coli K-12 by a recombinant plasmid containing the dam' gene by Arraj J. A. (1983)
  3. Bachmann B. J. 1987. Derivations and genotypes of some mutant derivatives of Escherichia coli K-12 p. 1190-1219. In F. C. Neidhardt J. L. Ingraham K. B. Low B. Magasanik M. Schaechter and H. E. Umbarger (ed.) Escherichia coli and Salmonella typhimurium: cellular and molecular biology. American Society for Microbiology Washington D.C.
  4. Bale , A. , M. d' Alarcao , and M. G. Marinus . 1979 . Characterization of DNA adenine methylation mutants of Escherichia coli K-12 . Mutat. Res. 59 : 157 - 165 . (10.1016/0027-5107(79)90153-2) / Mutat. Res. / Characterization of DNA adenine methylation mutants of Escherichia coli K-12 by Bale A. (1979)
  5. Barany G. and R. B. Merrifield. 1980. Solid-phase peptide synthesis p. 1-284. In E. Gross and J. Meienhofer (ed.) The peptides vol. 2. Academic Press Inc. New York.
  6. Barras , F. , and M. G. Marinus . 1988 . Arrangement of Dam methylation sites (GATC) in the Escherichia coli chromosome . Nucleic Acids Res. 16 : 9821 - 9838 . (10.1093/nar/16.20.9821) / Nucleic Acids Res. / Arrangement of Dam methylation sites (GATC) in the Escherichia coli chromosome by Barras F. (1988)
  7. Boye , E. , and A. L0bner- Olesen . 1990 . The role of dam methyltransferase in the control of DNA replication in E. coli . Cell 62 : 981 - 989 . (10.1016/0092-8674(90)90272-G) / Cell / The role of dam methyltransferase in the control of DNA replication in E. coli by Boye E. (1990)
  8. Boye , E. , and A. L0bner- Olesen . 1991 . Bacterial growth studied by flow cytometry . Res. Microbiol. 142 : 131 - 135 . (10.1016/0923-2508(91)90020-B) / Res. Microbiol. / Bacterial growth studied by flow cytometry by Boye E. (1991)
  9. Boye , E. , K. Skarstad , and A. L0bner- Olesen . 1988 . Timing of chromosomal replication in Escherichia coli . Biochim. Biophys. Acta 951 : 359 - 364 . (10.1016/0167-4781(88)90107-8) / Biochim. Biophys. Acta / Timing of chromosomal replication in Escherichia coli by Boye E. (1988)
  10. Bradford , M. M. 1976 . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding . Anal. Biochem. 72 : 248 - 254 . (10.1016/0003-2697(76)90527-3) / Anal. Biochem. / A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding by Bradford M. M. (1976)
  11. Bremer , H. , and G. Churchward . 1977 . An examination of the Cooper-Helmstetter theory of DNA replication and its underlying assumptions . J. Theor. Biol. 69 : 645 - 654 . (10.1016/0022-5193(77)90373-3) / J. Theor. Biol. / An examination of the Cooper-Helmstetter theory of DNA replication and its underlying assumptions by Bremer H. (1977)
  12. Bremer H. and P. P. Dennis. 1987. Modulation of chemical composition and other parameters of the cell by growth rate p. 1527-1542. In F. C. Neidhardt J. L. Ingraham K. B. Low B. Magasanik M. Schaechter and H. E. Umbarger (ed.) Escherichia coli and Salmonella typhimurium: cellular and molecular biology. American Society for Microbiology Washington D.C.
  13. Campbell , J. , and N. Kleckner . 1990 . E. coli oriC and the dnaA gene promoter are sequestered from dam methyltransferase following the passage of the chromosomal replication fork . Cell 62 : 967 - 979 . (10.1016/0092-8674(90)90271-F) / Cell / E. coli oriC and the dnaA gene promoter are sequestered from dam methyltransferase following the passage of the chromosomal replication fork by Campbell J. (1990)
  14. Carraway R. E. 1979. Neurotensin and related substances p. 139-169. In B. M. Jaffe and H. R. Behrman (ed.) Methods in hormone radioimmunoassay. Academic Press Inc. New York. (10.1016/B978-0-12-379260-0.50015-7)
  15. Casadaban , M. J. , and S. N. Cohen . 1980 . Analysis of gene control signals by DNA fusion and cloning in Escherichia coli . J. Mol. Biol. 138 : 179 - 207 . (10.1016/0022-2836(80)90283-1) / J. Mol. Biol. / Analysis of gene control signals by DNA fusion and cloning in Escherichia coli by Casadaban M. J. (1980)
  16. Clark , D. J. , and 0. Maal 0e. 1967 . DNA replication and the division cycle in Eschenchia coli . J. Mol. Biol. 23 : 99 - 112 . (10.1016/S0022-2836(67)80070-6) / J. Mol. Biol. / DNA replication and the division cycle in Eschenchia coli by Clark D. J. (1967)
  17. Glickman , B. W. , P. Vanden Elsen , and M. Radman . 1978 . Increased mutagenesis in dam- mutants of Escherichia coli, a role of 6-methyladenine residues in mutation avoidance . Mol. Gen. Genet. 163 : 307 - 312 . (10.1007/BF00271960) / Mol. Gen. Genet. / Increased mutagenesis in dam- mutants of Escherichia coli, a role of 6-methyladenine residues in mutation avoidance by Glickman B. W. (1978)
  18. Hattman , S. M. , J. E. Brooks , and M. Masurekar . 1978 . Sequence specificity of the P1 modification methylase (M. Eco dam) controlled by the Escherichia coli dam gene . J. Mol. Biol. 126 : 367 - 380 . (10.1016/0022-2836(78)90046-3) / J. Mol. Biol. / Sequence specificity of the P1 modification methylase (M. Eco dam) controlled by the Escherichia coli dam gene by Hattman S. M. (1978)
  19. Herman , G. E. , and P. Modrich . 1981 . Escherichia coli K-12 clones that overproduce dam methylase are hypermutable . J. Bacteriol. 145 : 644 - 646 . (10.1128/JB.145.1.644-646.1981) / J. Bacteriol. / Escherichia coli K-12 clones that overproduce dam methylase are hypermutable by Herman G. E. (1981)
  20. Herman , G. E. , and P. Modrich . 1982 . Eschenichia coli dam methylase. Physical and catalytic properties of the homogeneous enzyme . J. Biol. Chem. 257 : 2605 - 2612 . (10.1016/S0021-9258(18)34967-6) / J. Biol. Chem. / Eschenichia coli dam methylase. Physical and catalytic properties of the homogeneous enzyme by Herman G. E. (1982)
  21. Hopp , T. P. , and K. R. Woods . 1981 . Prediction of protein antigenic determinants from amino acid sequences . Proc. Natl. Acad. Sci. USA 78 : 3824 - 3828 . (10.1073/pnas.78.6.3824) / Proc. Natl. Acad. Sci. USA / Prediction of protein antigenic determinants from amino acid sequences by Hopp T. P. (1981)
  22. Jonczyk P. R. Hines and D. W. Smith. 1989. The Escherichia NOTES 1685
  23. Lacks , S. , and B. Greenberg . 1977 . Complementary specificity of restriction endonucleases of Diplococcus pneumoniae with respect to DNA methylation . J. Mol. Biol. 114 : 153 - 168 . (10.1016/0022-2836(77)90289-3) / J. Mol. Biol. / Complementary specificity of restriction endonucleases of Diplococcus pneumoniae with respect to DNA methylation by Lacks S. (1977)
  24. Laemmli , U. K. 1970 . Cleavage of structural proteins during the assembly of the head of bacteriophage T4 . Nature (London) 227 : 680 - 685 . (10.1038/227680a0) / Nature (London) / Cleavage of structural proteins during the assembly of the head of bacteriophage T4 by Laemmli U. K. (1970)
  25. L0bner-Olesen A. E. Boye and M. G. Marinus. Multiple promoters control expression of the Eschenchia coli dam gene. Submitted for publication.
  26. Marinus , M. G. , A. Poteete , and J. Arraj . 1984 . Correlation of DNA adenine methylase activity with spontaneous mutability in Escherichia coli K-12 . Gene 28 : 123 - 125 . (10.1016/0378-1119(84)90095-7) / Gene / Correlation of DNA adenine methylase activity with spontaneous mutability in Escherichia coli K-12 by Marinus M. G. (1984)
  27. Modrich , P. 1987 . DNA mismatch correction. Annu . Rev. Biochem. 56 : 435 - 466 . (10.1146/annurev.bi.56.070187.002251) / Rev. Biochem. / DNA mismatch correction. Annu by Modrich P. (1987)
  28. Parker , B. , and M. G. Marinus . 1988 . A simple and rapid method to obtain substitution mutations in Escherichia coli: isolation of a dam deletion/insertion mutation . Gene 73 : 531 - 535 . (10.1016/0378-1119(88)90517-3) / Gene / A simple and rapid method to obtain substitution mutations in Escherichia coli: isolation of a dam deletion/insertion mutation by Parker B. (1988)
  29. Skarstad , K. , E. Boye , and H. B. Steen . 1986 . Timing of initiation of chromosome replication in individual Escherichia coli cells . EMBO J. 5 : 1711 - 1717 . (10.1002/j.1460-2075.1986.tb04415.x) / EMBO J. / Timing of initiation of chromosome replication in individual Escherichia coli cells by Skarstad K. (1986)
  30. Szyf , M. , K. Avraham-Haetzni , A. Reifman , J. Shlomai , F. Kaplan , A. Oppenheim , and A. Razin . 1984 . DNA methylation pattern is determined by the intracellular level of the methylase . Proc. Natl. Acad. Sci. USA 81 : 3278 - 3282 . (10.1073/pnas.81.11.3278) / Proc. Natl. Acad. Sci. USA / DNA methylation pattern is determined by the intracellular level of the methylase by Szyf M. (1984)
  31. Wenzel , C. , M. Moulard , A. L0bner- Olesen , and W. Guschlbauer . 1991 . Crosslinking of Dam methyltransferase with S-adenosyl-methionine . FEBS Lett. 280 : 147 - 151 . (10.1016/0014-5793(91)80224-Q) / FEBS Lett. / Crosslinking of Dam methyltransferase with S-adenosyl-methionine by Wenzel C. (1991)
Dates
Type When
Created 8 years, 10 months ago (Nov. 4, 2016, 3:52 p.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 1:16 p.m.)
Indexed 1 year, 1 month ago (July 31, 2024, 4:23 a.m.)
Issued 33 years, 6 months ago (March 1, 1992)
Published 33 years, 6 months ago (March 1, 1992)
Published Print 33 years, 6 months ago (March 1, 1992)
Funders 0

None

@article{Boye_1992, title={Quantitation of Dam methyltransferase in Escherichia coli}, volume={174}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.174.5.1682-1685.1992}, DOI={10.1128/jb.174.5.1682-1685.1992}, number={5}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Boye, E and Marinus, M G and Løbner-Olesen, A}, year={1992}, month=mar, pages={1682–1685} }