DOI: 10.1128/jb.174.15.5165-5167.1992. Construction and characterization of Streptomyces coelicolor A3(2) mutants that are multiply deficient in the nonessential hrd-encoded RNA polymerase sigma factors

Construction and characterization of Streptomyces coelicolor A3(2) mutants that are multiply deficient in the nonessential hrd-encoded RNA polymerase sigma factors

10.1128/jb.174.15.5165-5167.1992

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

Previous studies showed that Streptomyces coelicolor A3(2) has four genes (hrdA, hrdB, hrdC, and hrdD) that appear to encode RNA polymerase sigma factors very similar to the sigma 70 subunit of Escherichia coli and that hrdC and hrdD could be individually disrupted without causing obvious phenotypic defects. Here, hrdA was cloned and stable null hrdA and hrdD mutants were constructed by gene replacement. These two mutants and a previously constructed hrdC null mutant were used in crosses to generate hrdAC, hrdAD, hrdCD, and hrdACD strains. The inability to synthesize one, two, or all three of the nonessential hrd-encoded sigma factors had no obvious phenotypic consequences.

Bibliography

Buttner, M. J., & Lewis, C. G. (1992). Construction and characterization of Streptomyces coelicolor A3(2) mutants that are multiply deficient in the nonessential hrd-encoded RNA polymerase sigma factors. Journal of Bacteriology, 174(15), 5165â5167.

Authors 2

M J Buttner (first)
C G Lewis (additional)

References 15 Referenced 37

Angell S. C. G. Lewis M. J. Buttner and M. J. Bibb. Unpublished data.
la. Binnie , C. , M. Lampe , and R. Losic L 1986 . Gene encoding the (r3 species of RNA polymerase o factor from Bacillus subtilis . Proc. Natl. Acad. Sci. USA 83 : 5943 - 5947 . (10.1073/pnas.83.16.5943) / Proc. Natl. Acad. Sci. USA / Gene encoding the (r3 species of RNA polymerase o factor from Bacillus subtilis by Binnie C. (1986)
Brown , K. L. , S. Wood , and M. J. Buttner . 1992 . Isolation and characterization of the major vegetative RNA polymerase of Streptomyces coelicolor A3(2): renaturation of a sigma subunit using GroEL . Mol. Microbiol. 6 : 1133 - 1139 . (10.1111/j.1365-2958.1992.tb01551.x) / Mol. Microbiol. / Isolation and characterization of the major vegetative RNA polymerase of Streptomyces coelicolor A3(2): renaturation of a sigma subunit using GroEL by Brown K. L. (1992)
Buttner , M. J. 1989 . RNA polymerase heterogeneity in Streptomyces coelicolor A3(2) . Mol. Microbiol. 3 : 1653 - 1659 . (10.1111/j.1365-2958.1989.tb00151.x) / Mol. Microbiol. / RNA polymerase heterogeneity in Streptomyces coelicolor A3(2) by Buttner M. J. (1989)
Buttner , M. J. , K. F. Chater , and M. J. Bibb . 1990 . Cloning, disruption and transcriptional analysis of three RNA polymerase sigma factor genes in Streptomyces coelicolor A3(2) . J. Bacteriol. 172 : 3367 - 3378 . (10.1128/JB.172.6.3367-3378.1990) / J. Bacteriol. / Cloning, disruption and transcriptional analysis of three RNA polymerase sigma factor genes in Streptomyces coelicolor A3(2) by Buttner M. J. (1990)
Duncan , M. L. , S. S. Kalman , S. M. Thomas , and C. W. Price . 1987 . Gene encoding the 37,000-dalton minor sigma factor of Bacillus subtilis RNA polymerase: isolation, nucleotide sequence, chromosomal locus, and cryptic function . J. Bacteriol. 169 : 771 - 778 . (10.1128/JB.169.2.771-778.1987) / J. Bacteriol. / Gene encoding the 37,000-dalton minor sigma factor of Bacillus subtilis RNA polymerase: isolation, nucleotide sequence, chromosomal locus, and cryptic function by Duncan M. L. (1987)
Fisher , S. H. , C. J. Bruton , and K. F. Chater . 1987 . The glucose kinase gene of S. coelicolor and its use in selecting spontaneous deletions for desired regions of the chromosome . Mol. Gen. Genet. 206 : 35 - 44 . (10.1007/BF00326533) / Mol. Gen. Genet. / The glucose kinase gene of S. coelicolor and its use in selecting spontaneous deletions for desired regions of the chromosome by Fisher S. H. (1987)
Helmann , J. D. , and M. J. Chamberlin . 1988 . Structure and function of bacterial sigma factors. Annu . Rev. Biochem. 57 : 839 - 872 . (10.1146/annurev.bi.57.070188.004203) / Rev. Biochem. / Structure and function of bacterial sigma factors. Annu by Helmann J. D. (1988)
Ikeda , H. , E. T. Seno , C. J. Bruton , and K. F. Chater . 1984 . Genetic mapping, cloning and physiological aspects of the glucose kinase gene of Streptomyces coelicolor . Mol. Gen. Genet. 196 : 501 - 507 . (10.1007/BF00436199) / Mol. Gen. Genet. / Genetic mapping, cloning and physiological aspects of the glucose kinase gene of Streptomyces coelicolor by Ikeda H. (1984)
Janssen , G. R. , J. M. Ward , and M. J. Bibb . 1989 . Unusual transcriptional and translational features of the aminoglycoside phosphotransferase gene (aph) from Streptomyces fradiae . Genes Dev. 3 : 415 - 429 . (10.1101/gad.3.3.415) / Genes Dev. / Unusual transcriptional and translational features of the aminoglycoside phosphotransferase gene (aph) from Streptomyces fradiae by Janssen G. R. (1989)
Prentki , P. , and H. M. Krish . 1984 . In vitro insertional mutagenesis with a selectable DNA fragment . Gene 29 : 303 - 313 . (10.1016/0378-1119(84)90059-3) / Gene / In vitro insertional mutagenesis with a selectable DNA fragment by Prentki P. (1984)
Shiina , T. , K. Tanaka , and H. Takahashi . 1991 . Sequence of hrdB, an essential gene encoding sigma-like transcription factor of Streptomyces coelicolor A3(2): homology to principle sigma factors . Gene 107 : 145 - 148 . (10.1016/0378-1119(91)90308-X) / Gene / Sequence of hrdB, an essential gene encoding sigma-like transcription factor of Streptomyces coelicolor A3(2): homology to principle sigma factors by Shiina T. (1991)
Tanaka , K. , T. Shiina , and H. Takahashi . 1988 . Multiple principle sigma factor homologs in eubacteria: identification of the "rpoD box ." Science 242 : 1040 - 1042 . (10.1126/science.3194753) / Science / Multiple principle sigma factor homologs in eubacteria: identification of the "rpoD box by Tanaka K. (1988)
Tanaka , K. , T. Shiina , and H. Takahashi . 1991 . Nucleotide sequence of genes hrdA4, hrdC and hrdD from Streptomyces coelicolor A3(2) having similarity to rpoD genes . Mol. Gen. Genet. 229 : 334 - 340 . (10.1007/BF00267453) / Mol. Gen. Genet. / Nucleotide sequence of genes hrdA4, hrdC and hrdD from Streptomyces coelicolor A3(2) having similarity to rpoD genes by Tanaka K. (1991)
Zalacain , M. , A. Gonzalez , M. C. Guerrero , R. T. Mattaliano , F. Malpartida , and A. Jimenez . 1986 . Nucleotide sequence of the hygromycin B phosphotransferase gene from Streptomyces hygroscopicus . Nucleic Acids Res. 14 : 1565 - 1581 . (10.1093/nar/14.4.1565) / Nucleic Acids Res. / Nucleotide sequence of the hygromycin B phosphotransferase gene from Streptomyces hygroscopicus by Zalacain M. (1986)

Dates

Type	When
Created	8 years, 10 months ago (Nov. 7, 2016, 11:17 a.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:47 p.m.)
Indexed	1 month ago (Aug. 5, 2025, 8:14 a.m.)
Issued	33 years, 1 month ago (Aug. 1, 1992)
Published	33 years, 1 month ago (Aug. 1, 1992)
Published Print	33 years, 1 month ago (Aug. 1, 1992)

Funders 0

None

BibTeX

@article{Buttner_1992, title={Construction and characterization of Streptomyces coelicolor A3(2) mutants that are multiply deficient in the nonessential hrd-encoded RNA polymerase sigma factors}, volume={174}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.174.15.5165-5167.1992}, DOI={10.1128/jb.174.15.5165-5167.1992}, number={15}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Buttner, M J and Lewis, C G}, year={1992}, month=aug, pages={5165–5167} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        8,
        5
      ]
    ],
    "date-time": "2025-08-05T12:14:57Z",
    "timestamp": 1754396097211
  },
  "reference-count": 15,
  "publisher": "American Society for Microbiology",
  "issue": "15",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            1992,
            8,
            1
          ]
        ],
        "date-time": "1992-08-01T00:00:00Z",
        "timestamp": 712627200000
      },
      "content-version": "tdm",
      "delay-in-days": 0,
      "URL": "https://journals.asm.org/non-commercial-tdm-license"
    }
  ],
  "content-domain": {
    "domain": [
      "journals.asm.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1992,
        8
      ]
    ]
  },
  "abstract": "<jats:p>Previous studies showed that Streptomyces coelicolor A3(2) has four genes (hrdA, hrdB, hrdC, and hrdD) that appear to encode RNA polymerase sigma factors very similar to the sigma 70 subunit of Escherichia coli and that hrdC and hrdD could be individually disrupted without causing obvious phenotypic defects. Here, hrdA was cloned and stable null hrdA and hrdD mutants were constructed by gene replacement. These two mutants and a previously constructed hrdC null mutant were used in crosses to generate hrdAC, hrdAD, hrdCD, and hrdACD strains. The inability to synthesize one, two, or all three of the nonessential hrd-encoded sigma factors had no obvious phenotypic consequences.</jats:p>",
  "DOI": "10.1128/jb.174.15.5165-5167.1992",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2016,
        11,
        7
      ]
    ],
    "date-time": "2016-11-07T16:17:56Z",
    "timestamp": 1478535476000
  },
  "page": "5165-5167",
  "update-policy": "http://dx.doi.org/10.1128/asmj-crossmark-policy-page",
  "source": "Crossref",
  "is-referenced-by-count": 37,
  "title": "Construction and characterization of Streptomyces coelicolor A3(2) mutants that are multiply deficient in the nonessential hrd-encoded RNA polymerase sigma factors",
  "prefix": "10.1128",
  "volume": "174",
  "author": [
    {
      "given": "M J",
      "family": "Buttner",
      "sequence": "first",
      "affiliation": [
        {
          "name": "School of Biological Sciences, University of East Anglia, University Plain, Norwich, United Kingdom."
        }
      ]
    },
    {
      "given": "C G",
      "family": "Lewis",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "School of Biological Sciences, University of East Anglia, University Plain, Norwich, United Kingdom."
        }
      ]
    }
  ],
  "member": "235",
  "reference": [
    {
      "key": "p_1",
      "unstructured": "Angell S. C. G. Lewis M. J. Buttner and M. J. Bibb. Unpublished data."
    },
    {
      "key": "p_2",
      "doi-asserted-by": "crossref",
      "first-page": "5943",
      "DOI": "10.1073/pnas.83.16.5943",
      "article-title": "Gene encoding the (r3 species of RNA polymerase o factor from Bacillus subtilis",
      "volume": "83",
      "author": "Binnie C.",
      "year": "1986",
      "unstructured": "la. Binnie , C. , M. Lampe , and R. Losic L 1986 . Gene encoding the (r3 species of RNA polymerase o factor from Bacillus subtilis . Proc. Natl. Acad. Sci. USA 83 : 5943 - 5947 .",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "p_3",
      "doi-asserted-by": "crossref",
      "first-page": "1133",
      "DOI": "10.1111/j.1365-2958.1992.tb01551.x",
      "article-title": "Isolation and characterization of the major vegetative RNA polymerase of Streptomyces coelicolor A3(2): renaturation of a sigma subunit using GroEL",
      "volume": "6",
      "author": "Brown K. L.",
      "year": "1992",
      "unstructured": "Brown , K. L. , S. Wood , and M. J. Buttner . 1992 . Isolation and characterization of the major vegetative RNA polymerase of Streptomyces coelicolor A3(2): renaturation of a sigma subunit using GroEL . Mol. Microbiol. 6 : 1133 - 1139 .",
      "journal-title": "Mol. Microbiol."
    },
    {
      "key": "p_4",
      "doi-asserted-by": "crossref",
      "first-page": "1653",
      "DOI": "10.1111/j.1365-2958.1989.tb00151.x",
      "article-title": "RNA polymerase heterogeneity in Streptomyces coelicolor A3(2)",
      "volume": "3",
      "author": "Buttner M. J.",
      "year": "1989",
      "unstructured": "Buttner , M. J. 1989 . RNA polymerase heterogeneity in Streptomyces coelicolor A3(2) . Mol. Microbiol. 3 : 1653 - 1659 .",
      "journal-title": "Mol. Microbiol."
    },
    {
      "key": "p_5",
      "doi-asserted-by": "crossref",
      "first-page": "3367",
      "DOI": "10.1128/JB.172.6.3367-3378.1990",
      "article-title": "Cloning, disruption and transcriptional analysis of three RNA polymerase sigma factor genes in Streptomyces coelicolor A3(2)",
      "volume": "172",
      "author": "Buttner M. J.",
      "year": "1990",
      "unstructured": "Buttner , M. J. , K. F. Chater , and M. J. Bibb . 1990 . Cloning, disruption and transcriptional analysis of three RNA polymerase sigma factor genes in Streptomyces coelicolor A3(2) . J. Bacteriol. 172 : 3367 - 3378 .",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "p_6",
      "doi-asserted-by": "crossref",
      "first-page": "771",
      "DOI": "10.1128/JB.169.2.771-778.1987",
      "article-title": "Gene encoding the 37,000-dalton minor sigma factor of Bacillus subtilis RNA polymerase: isolation, nucleotide sequence, chromosomal locus, and cryptic function",
      "volume": "169",
      "author": "Duncan M. L.",
      "year": "1987",
      "unstructured": "Duncan , M. L. , S. S. Kalman , S. M. Thomas , and C. W. Price . 1987 . Gene encoding the 37,000-dalton minor sigma factor of Bacillus subtilis RNA polymerase: isolation, nucleotide sequence, chromosomal locus, and cryptic function . J. Bacteriol. 169 : 771 - 778 .",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "p_7",
      "doi-asserted-by": "crossref",
      "first-page": "35",
      "DOI": "10.1007/BF00326533",
      "article-title": "The glucose kinase gene of S. coelicolor and its use in selecting spontaneous deletions for desired regions of the chromosome",
      "volume": "206",
      "author": "Fisher S. H.",
      "year": "1987",
      "unstructured": "Fisher , S. H. , C. J. Bruton , and K. F. Chater . 1987 . The glucose kinase gene of S. coelicolor and its use in selecting spontaneous deletions for desired regions of the chromosome . Mol. Gen. Genet. 206 : 35 - 44 .",
      "journal-title": "Mol. Gen. Genet."
    },
    {
      "key": "p_8",
      "doi-asserted-by": "crossref",
      "first-page": "839",
      "DOI": "10.1146/annurev.bi.57.070188.004203",
      "article-title": "Structure and function of bacterial sigma factors. Annu",
      "volume": "57",
      "author": "Helmann J. D.",
      "year": "1988",
      "unstructured": "Helmann , J. D. , and M. J. Chamberlin . 1988 . Structure and function of bacterial sigma factors. Annu . Rev. Biochem. 57 : 839 - 872 .",
      "journal-title": "Rev. Biochem."
    },
    {
      "key": "p_9",
      "doi-asserted-by": "crossref",
      "first-page": "501",
      "DOI": "10.1007/BF00436199",
      "article-title": "Genetic mapping, cloning and physiological aspects of the glucose kinase gene of Streptomyces coelicolor",
      "volume": "196",
      "author": "Ikeda H.",
      "year": "1984",
      "unstructured": "Ikeda , H. , E. T. Seno , C. J. Bruton , and K. F. Chater . 1984 . Genetic mapping, cloning and physiological aspects of the glucose kinase gene of Streptomyces coelicolor . Mol. Gen. Genet. 196 : 501 - 507 .",
      "journal-title": "Mol. Gen. Genet."
    },
    {
      "key": "p_10",
      "doi-asserted-by": "crossref",
      "first-page": "415",
      "DOI": "10.1101/gad.3.3.415",
      "article-title": "Unusual transcriptional and translational features of the aminoglycoside phosphotransferase gene (aph) from Streptomyces fradiae",
      "volume": "3",
      "author": "Janssen G. R.",
      "year": "1989",
      "unstructured": "Janssen , G. R. , J. M. Ward , and M. J. Bibb . 1989 . Unusual transcriptional and translational features of the aminoglycoside phosphotransferase gene (aph) from Streptomyces fradiae . Genes Dev. 3 : 415 - 429 .",
      "journal-title": "Genes Dev."
    },
    {
      "key": "p_11",
      "doi-asserted-by": "crossref",
      "first-page": "303",
      "DOI": "10.1016/0378-1119(84)90059-3",
      "article-title": "In vitro insertional mutagenesis with a selectable DNA fragment",
      "volume": "29",
      "author": "Prentki P.",
      "year": "1984",
      "unstructured": "Prentki , P. , and H. M. Krish . 1984 . In vitro insertional mutagenesis with a selectable DNA fragment . Gene 29 : 303 - 313 .",
      "journal-title": "Gene"
    },
    {
      "key": "p_12",
      "doi-asserted-by": "crossref",
      "first-page": "145",
      "DOI": "10.1016/0378-1119(91)90308-X",
      "article-title": "Sequence of hrdB, an essential gene encoding sigma-like transcription factor of Streptomyces coelicolor A3(2): homology to principle sigma factors",
      "volume": "107",
      "author": "Shiina T.",
      "year": "1991",
      "unstructured": "Shiina , T. , K. Tanaka , and H. Takahashi . 1991 . Sequence of hrdB, an essential gene encoding sigma-like transcription factor of Streptomyces coelicolor A3(2): homology to principle sigma factors . Gene 107 : 145 - 148 .",
      "journal-title": "Gene"
    },
    {
      "key": "p_13",
      "doi-asserted-by": "crossref",
      "first-page": "1040",
      "DOI": "10.1126/science.3194753",
      "article-title": "Multiple principle sigma factor homologs in eubacteria: identification of the \"rpoD box",
      "volume": "242",
      "author": "Tanaka K.",
      "year": "1988",
      "unstructured": "Tanaka , K. , T. Shiina , and H. Takahashi . 1988 . Multiple principle sigma factor homologs in eubacteria: identification of the \"rpoD box .\" Science 242 : 1040 - 1042 .",
      "journal-title": "Science"
    },
    {
      "key": "p_14",
      "doi-asserted-by": "crossref",
      "first-page": "334",
      "DOI": "10.1007/BF00267453",
      "article-title": "Nucleotide sequence of genes hrdA4, hrdC and hrdD from Streptomyces coelicolor A3(2) having similarity to rpoD genes",
      "volume": "229",
      "author": "Tanaka K.",
      "year": "1991",
      "unstructured": "Tanaka , K. , T. Shiina , and H. Takahashi . 1991 . Nucleotide sequence of genes hrdA4, hrdC and hrdD from Streptomyces coelicolor A3(2) having similarity to rpoD genes . Mol. Gen. Genet. 229 : 334 - 340 .",
      "journal-title": "Mol. Gen. Genet."
    },
    {
      "key": "p_15",
      "doi-asserted-by": "crossref",
      "first-page": "1565",
      "DOI": "10.1093/nar/14.4.1565",
      "article-title": "Nucleotide sequence of the hygromycin B phosphotransferase gene from Streptomyces hygroscopicus",
      "volume": "14",
      "author": "Zalacain M.",
      "year": "1986",
      "unstructured": "Zalacain , M. , A. Gonzalez , M. C. Guerrero , R. T. Mattaliano , F. Malpartida , and A. Jimenez . 1986 . Nucleotide sequence of the hygromycin B phosphotransferase gene from Streptomyces hygroscopicus . Nucleic Acids Res. 14 : 1565 - 1581 .",
      "journal-title": "Nucleic Acids Res."
    }
  ],
  "container-title": "Journal of Bacteriology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://journals.asm.org/doi/pdf/10.1128/jb.174.15.5165-5167.1992",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://journals.asm.org/doi/pdf/10.1128/jb.174.15.5165-5167.1992",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        7,
        29
      ]
    ],
    "date-time": "2021-07-29T17:47:53Z",
    "timestamp": 1627580873000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://journals.asm.org/doi/10.1128/jb.174.15.5165-5167.1992"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1992,
        8
      ]
    ]
  },
  "references-count": 15,
  "journal-issue": {
    "issue": "15",
    "published-print": {
      "date-parts": [
        [
          1992,
          8
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1128/jb.174.15.5165-5167.1992"
  ],
  "URL": "http://dx.doi.org/10.1128/jb.174.15.5165-5167.1992",
  "relation": {},
  "ISSN": [
    "0021-9193",
    "1098-5530"
  ],
  "subject": [],
  "container-title-short": "J Bacteriol",
  "published": {
    "date-parts": [
      [
        1992,
        8
      ]
    ]
  },
  "assertion": [
    {
      "value": "2021-02-02",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}