In vitro repair of double-strand breaks accompanied by recombination in bacteriophage T7 DNA
10.1128/jb.174.1.155-160.1992
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

A double-strand break in a bacteriophage T7 genome significantly reduced the ability of that DNA to produce viable phage when the DNA was incubated in an in vitro DNA replication and packaging system. When a homologous piece of T7 DNA (either a restriction fragment or T7 DNA cloned into a plasmid) that was by itself unable to form a complete phage was included in the reaction, the break was repaired to the extent that many more viable phage were produced. Moreover, repair could be completed even when a gap of about 900 nucleotides was put in the genome by two nearby restriction cuts. The repair was accompanied by acquisition of a genetic marker that was present only on the restriction fragment or on the T7 DNA cloned into a plasmid. These data are interpreted in light of the double-strand gap repair mode of recombination.

Bibliography

Masker, W. (1992). In vitro repair of double-strand breaks accompanied by recombination in bacteriophage T7 DNA. Journal of Bacteriology, 174(1), 155–160.

Authors 1
  1. W Masker (first)
References 43 Referenced 10
  1. De Massay , B. , R. A. Weisberg , and F. W. Studier . 1987 . Gene 3 endonuclease of bacteriophage T7 resolves conformationally branched structures in double-stranded DNA . J. Mol. Biol. 193 : 359 - 376 . (10.1016/0022-2836(87)90224-5) / J. Mol. Biol. / Gene 3 endonuclease of bacteriophage T7 resolves conformationally branched structures in double-stranded DNA by De Massay B. (1987)
  2. Dunn , J. H. , and F. W. Studier . 1983 . The complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements . J. Mol. Biol. 166 : 477 - 535 . (10.1016/S0022-2836(83)80282-4) / J. Mol. Biol. / The complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements by Dunn J. H. (1983)
  3. Hausmann , R. , B. Gomez , and B. Moody . 1968 . Physiological and genetic aspects of abortive infection of a Shigella sonnei strain by coliphage T7 . J. Virol. 2 : 335 - 345 . (10.1128/JVI.2.4.335-345.1968) / J. Virol. / Physiological and genetic aspects of abortive infection of a Shigella sonnei strain by coliphage T7 by Hausmann R. (1968)
  4. Hinkle , D. C. , and C. C. Richardson . 1974 . Bacteriophage T7 deoxyribonucleic acid replication in vitro. Requirements for deoxyribonucleic acid synthesis and characterization of the product . J. Biol. Chem. 249 : 2974 - 2984 . (10.1016/S0021-9258(19)42726-9) / J. Biol. Chem. / Bacteriophage T7 deoxyribonucleic acid replication in vitro. Requirements for deoxyribonucleic acid synthesis and characterization of the product by Hinkle D. C. (1974)
  5. Kerr , C. , and P. D. Sadowski . 1972 . Gene 6 exonuclease of bacteriophage T7. II. Mechanism of the reaction . J. Biol. Chem. 247 : 311 - 318 . (10.1016/S0021-9258(19)45791-8) / J. Biol. Chem. / Gene 6 exonuclease of bacteriophage T7. II. Mechanism of the reaction by Kerr C. (1972)
  6. Kerr , C. , and P. D. Sadowski . 1974 . Packaging and maturation of bacteriophage T7 in vitro . Proc. Natl. Acad. Sci. USA 71 : 3545 - 3549 . (10.1073/pnas.71.9.3545) / Proc. Natl. Acad. Sci. USA / Packaging and maturation of bacteriophage T7 in vitro by Kerr C. (1974)
  7. Kerr , C. , and P. D. Sadowski . 1975 . The involvement of genes 3, 4, 5, and 6 in genetic recombination in bacteriophage T7 . Virology 65 : 281 - 285 . (10.1016/0042-6822(75)90031-8) / Virology / The involvement of genes 3, 4, 5, and 6 in genetic recombination in bacteriophage T7 by Kerr C. (1975)
  8. Kuemmerle , N. B. , and W. E. Masker . 1977 . In vitro packaging of UV radiation-damaged DNA from bacteriophage T7 . J. Virol. 23 : 509 - 516 . (10.1128/JVI.23.3.509-516.1977) / J. Virol. / In vitro packaging of UV radiation-damaged DNA from bacteriophage T7 by Kuemmerle N. B. (1977)
  9. Lee , D. , and P. D. Sadowski . 1983 . In vitro recombination of bacteriophage T7 DNA detected by a direct physical assay . J. Virol. 48 : 647 - 653 . (10.1128/JVI.48.3.647-653.1983) / J. Virol. / In vitro recombination of bacteriophage T7 DNA detected by a direct physical assay by Lee D. (1983)
  10. Lee , D. , D. Vetter , L. Beatty , and P. Sadowski . 1985 . In vitro recombination of bacteriophage T7 DNA: further characterization of the reaction using plasmid DNA . Can. J. Biochem. Cell Biol. 63 : 243 - 248 . (10.1139/o85-035) / Can. J. Biochem. Cell Biol. / In vitro recombination of bacteriophage T7 DNA: further characterization of the reaction using plasmid DNA by Lee D. (1985)
  11. Lee , M. , and R. C. Miller , Jr. 1974 . T7 exonuclease (GENE 6) is necessary for molecular recombination of bacteriophage T7 . J. Virol. 14 : 1040 - 1048 . (10.1128/JVI.14.5.1040-1048.1974) / J. Virol. / T7 exonuclease (GENE 6) is necessary for molecular recombination of bacteriophage T7 by Lee M. (1974)
  12. Lee , M. , R. C. Miller , Jr. , D. Scraba , and V. Paetkau . 1976 . The essential role of bacteriophage T7 endonuclease (gene 3) in molecular recombination . J. Mol. Biol. 104 : 883 - 888 . (10.1016/0022-2836(76)90189-3) / J. Mol. Biol. / The essential role of bacteriophage T7 endonuclease (gene 3) in molecular recombination by Lee M. (1976)
  13. Masker , W. E. 1982 . In vitro packaging of bacteriophage T7 DNA requires ATP . J. Virol. 43 : 365 - 367 . (10.1128/JVI.43.1.365-367.1982) / J. Virol. / In vitro packaging of bacteriophage T7 DNA requires ATP by Masker W. E. (1982)
  14. Masker , W. E. , and N. B. Kuemmerle . 1980 . In vitro recombination of bacteriophage T7 DNA damaged by UV radiation . J. Virol. 33 : 330 - 339 . (10.1128/JVI.33.1.330-339.1980) / J. Virol. / In vitro recombination of bacteriophage T7 DNA damaged by UV radiation by Masker W. E. (1980)
  15. Masker , W. E. , N. B. Kuemmerle , and D. P. Allison . 1978 . In vitro packaging of bacteriophage T7 DNA synthesized in vitro . J. Virol. 27 : 149 - 163 . (10.1128/JVI.27.1.149-163.1978) / J. Virol. / In vitro packaging of bacteriophage T7 DNA synthesized in vitro by Masker W. E. (1978)
  16. Miller J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.
  17. Mosig G. 1988. Mapping and map distortions in bacteriophage crosses p. 141-167. In R. Kucherlapati and G. R. Smith (ed.) Genetic recombination. American Society for Microbiology Washington D.C.
  18. Muller , B. , C. Jones , and S. C. West . 1990 . T7 endonuclease I resolves Holliday junctions formed in vitro by RecA protein . Nucleic Acids Res. 18 : 5633 - 5636 . (10.1093/nar/18.19.5633) / Nucleic Acids Res. / T7 endonuclease I resolves Holliday junctions formed in vitro by RecA protein by Muller B. (1990)
  19. Pierce , J. C. , and W. Masker . 1989 . Genetic deletions between directly repeated sequences in bacteriophage T7 . Mol. Gen. Genet. 217 : 215 - 222 . (10.1007/BF02464884) / Mol. Gen. Genet. / Genetic deletions between directly repeated sequences in bacteriophage T7 by Pierce J. C. (1989)
  20. Pierce , J. C. , and W. E. Masker . 1988 . A single-base change in gene 10 of bacteriophage T7 permits growth on Shigella sonnei . J. Virol. 62 : 4369 - 4371 . (10.1128/JVI.62.11.4369-4371.1988) / J. Virol. / A single-base change in gene 10 of bacteriophage T7 permits growth on Shigella sonnei by Pierce J. C. (1988)
  21. Powling , A. , and R. Knippers . 1974 . Some functions involved in bacteriophage T7 genetic recombination . Mol. Gen. Genet. 134 : 173 - 180 . (10.1007/BF00268418) / Mol. Gen. Genet. / Some functions involved in bacteriophage T7 genetic recombination by Powling A. (1974)
  22. Powling , A. , and R. Knippers . 1976 . Recombination of bacteriophage T7 in vivo . Mol. Gen. Genet. 149 : 63 - 71 . (10.1007/BF00275961) / Mol. Gen. Genet. / Recombination of bacteriophage T7 in vivo by Powling A. (1976)
  23. Resnick , M. 1976 . The repair of double strand breaks in DNA: a model involving recombination . J. Theor. Biol. 59 : 97 - 106 . (10.1016/S0022-5193(76)80025-2) / J. Theor. Biol. / The repair of double strand breaks in DNA: a model involving recombination by Resnick M. (1976)
  24. Richardson , C. C. 1966 . The 5'-terminal nucleotides of bacteriophage T7 deoxyribonucleic Acid . J. Mol. Biol. 15 : 49 - 61 . (10.1016/S0022-2836(66)80208-5) / J. Mol. Biol. / The 5'-terminal nucleotides of bacteriophage T7 deoxyribonucleic Acid by Richardson C. C. (1966)
  25. Roberts , L. , R. Sheldon , and P. D. Sadowski . 1978 . Genetic recombination of bacteriophage T7 DNA in vitro. IV. Asymmetry of recombination frequencies caused by polarity of DNA packaging in vitro . Virology 89 : 252 - 261 . (10.1016/0042-6822(78)90057-0) / Virology / Genetic recombination of bacteriophage T7 DNA in vitro. IV. Asymmetry of recombination frequencies caused by polarity of DNA packaging in vitro by Roberts L. (1978)
  26. Roeder , G. S. , and P. D. Sadowski . 1979 . Pathways of recombination of bacteriophage T7 DNA in vitro . Cold Spring Harbor Symp. Quant. Biol. 43 : 1023 - 1032 . (10.1101/SQB.1979.043.01.111) / Cold Spring Harbor Symp. Quant. Biol. / Pathways of recombination of bacteriophage T7 DNA in vitro by Roeder G. S. (1979)
  27. Sadowski , P. D. 1977 . Genetic recombination of bacteriophage T7 DNA in vitro. II. Further properties of the in vitro recombination-packaging reaction . Virology 78 : 192 - 202 . (10.1016/0042-6822(77)90091-5) / Virology / Genetic recombination of bacteriophage T7 DNA in vitro. II. Further properties of the in vitro recombination-packaging reaction by Sadowski P. D. (1977)
  28. Sadowski P. D. W. Bradley D. Lee and L. Roberts. 1980. Genetic recombination of bacteriophage T7 DNA in vitro p. 941-952. In B. Alberts and C. F. Fox (ed.) Molecular mechanisms of replication and genetic recombination. Academic Press Inc. New York. (10.1016/B978-0-12-048850-6.50085-5)
  29. Sadowski , P. D. , and D. Vetter . 1976 . Genetic recombination of bacteriophage T7 DNA in vitro . Proc. Natl. Acad. Sci. USA 73 : 692 - 696 . (10.1073/pnas.73.3.692) / Proc. Natl. Acad. Sci. USA / Genetic recombination of bacteriophage T7 DNA in vitro by Sadowski P. D. (1976)
  30. Sambrook J. E. F. Fritsch and T. Maniatis. 1989. Molecular cloning 2nd ed. Cold Spring Harbor Laboratory Press Cold Spring Harbor N.Y.
  31. Serwer , P. , W. E. Masker , and J. L. Allen . 1983 . Stability and in vitro DNA packaging of bacteriophages: effects of dextrans, sugars, and polyols . J. Virol. 45 : 665 - 671 . (10.1128/JVI.45.2.665-671.1983) / J. Virol. / Stability and in vitro DNA packaging of bacteriophages: effects of dextrans, sugars, and polyols by Serwer P. (1983)
  32. Smith , R. D. , and R. C. Miller , Jr. 1981 . Replication and plasmid-bacteriophage recombination. I. Marker rescue analysis . Virology 115 : 223 - 236 . (10.1016/0042-6822(81)90106-9) / Virology / Replication and plasmid-bacteriophage recombination. I. Marker rescue analysis by Smith R. D. (1981)
  33. Stone , J. C. , and R. C. Miller , Jr. 1984 . Plasmid-phage recombination in T7 infected Escherichia coli . Virology 137 : 305 - 313 . (10.1016/0042-6822(84)90222-8) / Virology / Plasmid-phage recombination in T7 infected Escherichia coli by Stone J. C. (1984)
  34. Studier , F. W. 1969 . The genetics and physiology of bacteriophage T7 . Virology 39 : 562 - 574 . (10.1016/0042-6822(69)90104-4) / Virology / The genetics and physiology of bacteriophage T7 by Studier F. W. (1969)
  35. Studier , F. W. 1972 . Bacteriophage T7 . Science 176 : 367 - 376 . (10.1126/science.176.4033.367) / Science / Bacteriophage T7 by Studier F. W. (1972)
  36. Symington , L. S. 1991 . Double-strand-break repair and recombination catalyzed by a nuclear extract of Saccharomyces cerevisiae . EMBO J. 10 : 987 - 996 . (10.1002/j.1460-2075.1991.tb08033.x) / EMBO J. / Double-strand-break repair and recombination catalyzed by a nuclear extract of Saccharomyces cerevisiae by Symington L. S. (1991)
  37. Szostak , J. W. , T. L. Orr-Weaver , R. Rothstein , and F. W. Stahl . 1983 . The double-strand-break repair model for recombination . Cell 33 : 25 - 35 . (10.1016/0092-8674(83)90331-8) / Cell / The double-strand-break repair model for recombination by Szostak J. W. (1983)
  38. Takahgshi , N. , and I. Kobayashi . 1990 . Evidence for the doublestrand break repair model of bacteriophage lambda recombination . Proc. Natl. Acad. Sci. USA 87 : 2790 - 2794 . (10.1073/pnas.87.7.2790) / Proc. Natl. Acad. Sci. USA / Evidence for the doublestrand break repair model of bacteriophage lambda recombination by Takahgshi N. (1990)
  39. Thaler , D. S. , and F. W. Stahl . 1988 . DNA double-chain breaks in recombination of phage lambda and of yeast. Annu . Rev. Genet. 22 : 169 - 197 . (10.1146/annurev.ge.22.120188.001125) / Rev. Genet. / DNA double-chain breaks in recombination of phage lambda and of yeast. Annu by Thaler D. S. (1988)
  40. Tsujimoto , Y. , and H. Ogawa . 1977 . Intermediates in genetic recombination of bacteriophage T7 DNA . J. Mol. Biol. 109 : 423 - 436 . (10.1016/S0022-2836(77)80021-1) / J. Mol. Biol. / Intermediates in genetic recombination of bacteriophage T7 DNA by Tsujimoto Y. (1977)
  41. Tsujimoto , Y. , and H. Ogawa . 1978 . Intermediates in genetic recombination of bacteriophage T7 DNA. Biological activity and the roles of gene 3 and gene 5 . J. Mol. Biol. 125 : 255 - 273 . (10.1016/0022-2836(78)90402-3) / J. Mol. Biol. / Intermediates in genetic recombination of bacteriophage T7 DNA. Biological activity and the roles of gene 3 and gene 5 by Tsujimoto Y. (1978)
  42. Valachopoulov , P. J. , and P. D. Sadowski . 1977 . Genetic recombination of bacteriophage T7 DNA in vitro. III. A physical assay for recombinant DNA . Virology 78 : 203 - 215 . (10.1016/0042-6822(77)90092-7) / Virology / Genetic recombination of bacteriophage T7 DNA in vitro. III. A physical assay for recombinant DNA by Valachopoulov P. J. (1977)
  43. Wackernagel , W. , and U. Hermanns . 1974 . Inhibition of exonuclease V after infection of E. coli by bacteriophage T7 . Biochem. Biophys. Res. Commun. 60 : 521 - 527 . (10.1016/0006-291X(74)90271-X) / Biochem. Biophys. Res. Commun. / Inhibition of exonuclease V after infection of E. coli by bacteriophage T7 by Wackernagel W. (1974)
Dates
Type When
Created 8 years, 9 months ago (Nov. 14, 2016, 11:46 a.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 1:47 p.m.)
Indexed 1 year, 2 months ago (July 2, 2024, 11:05 a.m.)
Issued 33 years, 8 months ago (Jan. 1, 1992)
Published 33 years, 8 months ago (Jan. 1, 1992)
Published Print 33 years, 8 months ago (Jan. 1, 1992)
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@article{Masker_1992, title={In vitro repair of double-strand breaks accompanied by recombination in bacteriophage T7 DNA}, volume={174}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.174.1.155-160.1992}, DOI={10.1128/jb.174.1.155-160.1992}, number={1}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Masker, W}, year={1992}, month=jan, pages={155–160} }