The FtsQ protein of Escherichia coli: membrane topology, abundance, and cell division phenotypes due to overproduction and insertion mutations
10.1128/jb.173.7.2187-2195.1991
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

The ftsQ gene is one of several genes thought to be specifically required for septum formation in Escherichia coli. Published work on the cell division behavior of ftsQ temperature-sensitive mutants suggested that the FtsQ product is required throughout the whole process of septum formation. Here we provide additional support for this hypothesis based on microscopic observations of the cell division defects resulting from insertional and temperature-sensitive mutations in the ftsQ gene, and constitutive overexpression of its gene product. On the basis of the published, predicted amino acid sequence of the FtsQ protein and our analysis of fusion proteins of the FtsQ protein to bacterial alkaline phosphatase, we conclude that FtsQ is a simple cytoplasmic membrane protein with a approximately 25-amino-acid cytoplasmic domain and a approximately 225-amino-acid periplasmic domain. We estimate that the FtsQ protein is present at about 22 copies per cell.

Bibliography

Carson, M. J., Barondess, J., & Beckwith, J. (1991). The FtsQ protein of Escherichia coli: membrane topology, abundance, and cell division phenotypes due to overproduction and insertion mutations. Journal of Bacteriology, 173(7), 2187–2195.

Authors 3
  1. M J Carson (first)
  2. J Barondess (additional)
  3. J Beckwith (additional)
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Dates
Type When
Created 8 years, 9 months ago (Nov. 7, 2016, 11:33 a.m.)
Deposited 4 years, 1 month ago (July 29, 2021, 1:46 p.m.)
Indexed 3 months, 1 week ago (May 21, 2025, 1:46 p.m.)
Issued 34 years, 5 months ago (April 1, 1991)
Published 34 years, 5 months ago (April 1, 1991)
Published Print 34 years, 5 months ago (April 1, 1991)
Funders 0

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@article{Carson_1991, title={The FtsQ protein of Escherichia coli: membrane topology, abundance, and cell division phenotypes due to overproduction and insertion mutations}, volume={173}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.173.7.2187-2195.1991}, DOI={10.1128/jb.173.7.2187-2195.1991}, number={7}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Carson, M J and Barondess, J and Beckwith, J}, year={1991}, month=apr, pages={2187–2195} }