DOI: 10.1128/jb.173.6.1873-1878.1991. Stoichiometry of maltodextrin-binding sites in LamB, an outer membrane protein from Escherichia coli

Stoichiometry of maltodextrin-binding sites in LamB, an outer membrane protein from Escherichia coli

10.1128/jb.173.6.1873-1878.1991

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

We have directly measured the stoichiometry of maltodextrin-binding sites in LamB. Scatchard plots and computer fitting of flow dialysis (rate-of-dialysis) experiments clearly establish three independent binding sites per LamB trimer, with a dissociation constant of approximately 60 microM for maltoheptaose. The current model for LamB's function as a specific pore is discussed with respect to the symmetry in LamB's kinetic properties and the implications of our results.

Bibliography

Gehring, K., Cheng, C. H., Nikaido, H., & Jap, B. K. (1991). Stoichiometry of maltodextrin-binding sites in LamB, an outer membrane protein from Escherichia coli. Journal of Bacteriology, 173(6), 1873â1878.

Authors 4

K Gehring (first)
C H Cheng (additional)
H Nikaido (additional)
B K Jap (additional)

References 28 Referenced 12

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Dates

Type	When
Created	8 years, 10 months ago (Nov. 4, 2016, 1:52 p.m.)
Deposited	2 years ago (Aug. 20, 2023, 3:25 p.m.)
Indexed	1 year, 8 months ago (Dec. 8, 2023, 3:32 a.m.)
Issued	34 years, 6 months ago (March 1, 1991)
Published	34 years, 6 months ago (March 1, 1991)
Published Print	34 years, 6 months ago (March 1, 1991)

Funders 0

None

BibTeX

@article{Gehring_1991, title={Stoichiometry of maltodextrin-binding sites in LamB, an outer membrane protein from Escherichia coli}, volume={173}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.173.6.1873-1878.1991}, DOI={10.1128/jb.173.6.1873-1878.1991}, number={6}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Gehring, K and Cheng, C H and Nikaido, H and Jap, B K}, year={1991}, month=mar, pages={1873–1878} }

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