DOI: 10.1128/jb.173.24.7867-7874.1991. Effect of chromosome location of Bacillus subtilis forespore genes on their spo gene dependence and transcription by E sigma F: identification of features of good E sigma F-dependent promoters

Effect of chromosome location of Bacillus subtilis forespore genes on their spo gene dependence and transcription by E sigma F: identification of features of good E sigma F-dependent promoters

10.1128/jb.173.24.7867-7874.1991

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

Translational lacZ fusions to forespore genes of Bacillus subtilis were not expressed in spoIIAC (sigma F) or spoIIIE mutants when the lacZ fusions were integrated at the loci of the same genes or at the SP beta locus. However, some of these genes, including gerA, gpr, spoIIIG (sigma G), and sspE, were expressed in spoIIIE mutants and spoIIIE spoIIIG double mutants (but not in spoIIAC mutants) when the lacZ fusions were integrated at the amyE locus. When tested, the beta-galactosidase made in these mutants was found only in the forespore, and the 5' ends of the mRNAs produced in these mutants were identical to those in a Spo+ background. Analysis of the in vitro transcription of forespore genes by RNA polymerase containing sigma F (E sigma F) revealed a direct correlation between good in vitro transcription by E sigma F and expression at the amyE locus in spoIIIE mutants. This result suggests that forespore genes are transcribed by E sigma F in spoIIIE and spoIIIE spoIIIG mutants. Comparison of the promoter regions of genes transcribed well and poorly by E sigma F in vivo and in vitro showed that good transcription by E sigma F was correlated with G residues at positions -15 and -16, a purine residue at position -13, and a T residue at position -7 relative to the start site of transcription. The importance of these residues in sigma F recognition was confirmed by analysis of the E sigma F-dependent transcription in vivo and in vitro of mutant ssp genes.

Bibliography

Sun, D., Fajardo-Cavazos, P., Sussman, M. D., Tovar-Rojo, F., Cabrera-Martinez, R. M., & Setlow, P. (1991). Effect of chromosome location of Bacillus subtilis forespore genes on their spo gene dependence and transcription by E sigma F: identification of features of good E sigma F-dependent promoters. Journal of Bacteriology, 173(24), 7867â7874.

Authors 6

D Sun (first)
P Fajardo-Cavazos (additional)
M D Sussman (additional)
F Tovar-Rojo (additional)
R M Cabrera-Martinez (additional)
P Setlow (additional)

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Dates

Type	When
Created	8 years, 9 months ago (Nov. 14, 2016, 11:55 a.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:13 p.m.)
Indexed	1 year, 3 months ago (May 16, 2024, 12:15 p.m.)
Issued	33 years, 9 months ago (Dec. 1, 1991)
Published	33 years, 9 months ago (Dec. 1, 1991)
Published Print	33 years, 9 months ago (Dec. 1, 1991)

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BibTeX

@article{Sun_1991, title={Effect of chromosome location of Bacillus subtilis forespore genes on their spo gene dependence and transcription by E sigma F: identification of features of good E sigma F-dependent promoters}, volume={173}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.173.24.7867-7874.1991}, DOI={10.1128/jb.173.24.7867-7874.1991}, number={24}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Sun, D and Fajardo-Cavazos, P and Sussman, M D and Tovar-Rojo, F and Cabrera-Martinez, R M and Setlow, P}, year={1991}, month=dec, pages={7867–7874} }

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  "abstract": "<jats:p>Translational lacZ fusions to forespore genes of Bacillus subtilis were not expressed in spoIIAC (sigma F) or spoIIIE mutants when the lacZ fusions were integrated at the loci of the same genes or at the SP beta locus. However, some of these genes, including gerA, gpr, spoIIIG (sigma G), and sspE, were expressed in spoIIIE mutants and spoIIIE spoIIIG double mutants (but not in spoIIAC mutants) when the lacZ fusions were integrated at the amyE locus. When tested, the beta-galactosidase made in these mutants was found only in the forespore, and the 5' ends of the mRNAs produced in these mutants were identical to those in a Spo+ background. Analysis of the in vitro transcription of forespore genes by RNA polymerase containing sigma F (E sigma F) revealed a direct correlation between good in vitro transcription by E sigma F and expression at the amyE locus in spoIIIE mutants. This result suggests that forespore genes are transcribed by E sigma F in spoIIIE and spoIIIE spoIIIG mutants. Comparison of the promoter regions of genes transcribed well and poorly by E sigma F in vivo and in vitro showed that good transcription by E sigma F was correlated with G residues at positions -15 and -16, a purine residue at position -13, and a T residue at position -7 relative to the start site of transcription. The importance of these residues in sigma F recognition was confirmed by analysis of the E sigma F-dependent transcription in vivo and in vitro of mutant ssp genes.</jats:p>",
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