Resolution of Holliday junctions in Escherichia coli: identification of the ruvC gene product as a 19-kilodalton protein
10.1128/jb.173.23.7711-7715.1991
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

The ruvC gene of Escherichia coli specifies a nuclease that resolves Holliday junction intermediates in genetic recombination (B. Connolly, C.A. Parsons, F.E. Benson, H.J. Dunderdale, G.J. Sharples, R.G. Lloyd, and S.C. West, Proc. Natl. Acad, Sci. USA 88:6063-6067, 1991). The gene was located between aspS and the ruvAB operon by DNA sequencing and deletion analysis of ruvC plasmids and was shown to encode a protein of 18,747 Da. Analysis of the DNA flanking ruvC indicated that the gene is transcribed independently of the LexA-regulated ruvAB operon and is not under direct SOS control. ruvC lies downstream of an open reading frame, orf-33, for a protein which migrates during sodium dodecyl sulfate-polyacrylamide gel electrophoresis as a 33-kDa polypeptide. These two genes probably form an operon. However, expression of ruvC was found to be very poor relative to that of orf-33. A double ribosomal frameshift between these genes is proposed as a possible reason for the low level of RuvC. Two further open reading frames of unknown function were identified, one on either side of the orf-33-ruvC operon.

Bibliography

Sharples, G. J., & Lloyd, R. G. (1991). Resolution of Holliday junctions in Escherichia coli: identification of the ruvC gene product as a 19-kilodalton protein. Journal of Bacteriology, 173(23), 7711–7715.

Authors 2
  1. G J Sharples (first)
  2. R G Lloyd (additional)
References 33 Referenced 69
  1. Attfield , P. V. , F. E. Benson , and R. G. Lloyd . 1985 . Analysis of the ruv locus of Escherichia coli K-12 and identification of the gene product . J. Bacteriol. 164 : 276 - 281 . (10.1128/jb.164.1.276-281.1985) / J. Bacteriol. / Analysis of the ruv locus of Escherichia coli K-12 and identification of the gene product by Attfield P. V. (1985)
  2. Bachmann B. J. 1987. Derivations and genotypes of some mutant derivatives of Escherichia coli K-12 p. 1190-1219. In F. C. Neidhardt J. L. Ingraham K. B. Low B. Magasanik M. Schaechter and H. E. Umbarger (ed.) Escherichia coli and Salmonella typhimurium: cellular and molecular biology. American Society for Microbiology Washington D.C.
  3. Barth , K. A. , D. Powell , M. Trupin , and G. Mosig . 1988 . Regulation of two nested proteins from gene 49 (recombination endonuclease VII) and of a X RexA-like protein of bacteriophage T4 . Genetics 120 : 329 - 343 . (10.1093/genetics/120.2.329) / Genetics / Regulation of two nested proteins from gene 49 (recombination endonuclease VII) and of a X RexA-like protein of bacteriophage T4 by Barth K. A. (1988)
  4. Benson , F. E. , G. T. Illing , G. J. Sharples , and R. G. Lloyd . 1988 . Nucleotide sequencing of the ruv region of Escherichia coli K-12 reveals a LexA regulated operon encoding two genes . Nucleic Acids Res. 16 : 1541 - 1549 . (10.1093/nar/16.4.1541) / Nucleic Acids Res. / Nucleotide sequencing of the ruv region of Escherichia coli K-12 reveals a LexA regulated operon encoding two genes by Benson F. E. (1988)
  5. Connolly , B. , C. Parsons , F. E. Benson , H. J. Dunderdale , G. J. Sharples , R. G. Lloyd , and S. C. West . 1991 . Resolution of Holliday junctions in vitro requires Escherichia coli ruvC gene product . Proc. Natl. Acad. Sci. USA 88 : 6063 - 6067 . (10.1073/pnas.88.14.6063) / Proc. Natl. Acad. Sci. USA / Resolution of Holliday junctions in vitro requires Escherichia coli ruvC gene product by Connolly B. (1991)
  6. Connolly , B. , and S. C. West . 1990 . Genetic recombination in E. coli: Holliday junctions made by RecA protein are resolved by fractionated cell-free extracts . Proc. Natl. Acad. Sci. USA 87 : 8476 - 8480 . (10.1073/pnas.87.21.8476) / Proc. Natl. Acad. Sci. USA / Genetic recombination in E. coli: Holliday junctions made by RecA protein are resolved by fractionated cell-free extracts by Connolly B. (1990)
  7. DeMassy , B. , R. A. Weisberg , and F. W. Studier . 1987 . Gene 3 endonuclease of bacteriophage T7 resolves conformationally branched structures in double-stranded DNA . J. Mol. Biol. 193 : 359 - 376 . (10.1016/0022-2836(87)90224-5) / J. Mol. Biol. / Gene 3 endonuclease of bacteriophage T7 resolves conformationally branched structures in double-stranded DNA by DeMassy B. (1987)
  8. Dickie , P. , G. McFadden , and A. R. Morgan . 1987 . The sitespecific cleavage of synthetic Holliday junction analogs and related branched DNA structures by bacteriophage T7 endonuclease I . J. Biol. Chem. 262 : 14826 - 14836 . (10.1016/S0021-9258(18)47870-2) / J. Biol. Chem. / The sitespecific cleavage of synthetic Holliday junction analogs and related branched DNA structures by bacteriophage T7 endonuclease I by Dickie P. (1987)
  9. Dunderdale H. J. F. E. Benson C. A. Parsons G. J. Sharples R. G. Lloyd and S. C. West. The formation and resolution of recombination intermediates by E. coli RecA and RuvC proteins. Nature (London) in press.
  10. Dunn , J. J. , and F. W. Studier . 1983 . Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements . J. Mol. Biol. 166 : 477 - 535 . (10.1016/S0022-2836(83)80282-4) / J. Mol. Biol. / Complete nucleotide sequence of bacteriophage T7 DNA and the locations of T7 genetic elements by Dunn J. J. (1983)
  11. Eriani , G. , G. Dirheimer , and J. Gangloff . 1990 . Aspartyl-tRNA synthetase from Escherichia coli: cloning and characterization of the gene, homologies of its translated amino acid sequence with asparaginyl- and Iysyl-tRNA synthetases . Nucleic Acids Res. 18 : 7109 - 7118 . (10.1093/nar/18.23.7109) / Nucleic Acids Res. / Aspartyl-tRNA synthetase from Escherichia coli: cloning and characterization of the gene, homologies of its translated amino acid sequence with asparaginyl- and Iysyl-tRNA synthetases by Eriani G. (1990)
  12. Howard-Flanders , P. , L. Theriot , and J. B. Stedeford . 1969 . Some properties of excision-defective recombination-deficient mutants of Escherichia coli K-12 . J. Bacteriol. 97 : 1134 - 1141 . (10.1128/jb.97.3.1134-1141.1969) / J. Bacteriol. / Some properties of excision-defective recombination-deficient mutants of Escherichia coli K-12 by Howard-Flanders P. (1969)
  13. Iwasaki , H. , T. Shiba , K. Makino , A. Nakata , and H. Shinagawa . 1989 . Overproduction, purification, and ATPase activity of the Escherichia coli RuvB protein involved in DNA repair . J. Bacteriol. 171 : 5276 - 5280 . (10.1128/jb.171.10.5276-5280.1989) / J. Bacteriol. / Overproduction, purification, and ATPase activity of the Escherichia coli RuvB protein involved in DNA repair by Iwasaki H. (1989)
  14. Kosak , H. G. , and B. W. Kemper . 1990 . Large-scale preparation of T4 endonuclease VII from over-expressing bacteria . Eur. J. Biochem. 194 : 779 - 784 . (10.1111/j.1432-1033.1990.tb19469.x) / Eur. J. Biochem. / Large-scale preparation of T4 endonuclease VII from over-expressing bacteria by Kosak H. G. (1990)
  15. Lloyd , R. G. 1991 . Conjugational recombination in resolvasedeficient ruvC mutants of Escherichia coli K-12 depends on recG . J. Bacteriol. 173 : 5414 - 5418 . (10.1128/jb.173.17.5414-5418.1991) / J. Bacteriol. / Conjugational recombination in resolvasedeficient ruvC mutants of Escherichia coli K-12 depends on recG by Lloyd R. G. (1991)
  16. Lloyd , R. G. , F. E. Benson , and C. E. Shurvinton . 1984 . Effect of ruv mutations on recombination and DNA repair in Escherichia coli K12 . Mol. Gen. Genet. 194 : 303 - 309 . (10.1007/BF00383532) / Mol. Gen. Genet. / Effect of ruv mutations on recombination and DNA repair in Escherichia coli K12 by Lloyd R. G. (1984)
  17. Lloyd , R. G. , C. Buckman , and F. E. Benson . 1987 . Genetic analysis of conjugational recombination in Escherichia coli K-12 strains deficient in RecBCD enzyme . J. Gen. Microbiol. 133 : 2531 - 2538 . / J. Gen. Microbiol. / Genetic analysis of conjugational recombination in Escherichia coli K-12 strains deficient in RecBCD enzyme by Lloyd R. G. (1987)
  18. Muller , B. , C. Jones , B. Kemper , and S. C. West . 1990 . Enzymatic formation and resolution of Holliday junctions in vitro . Cell 60 : 329 - 336 . (10.1016/0092-8674(90)90747-3) / Cell / Enzymatic formation and resolution of Holliday junctions in vitro by Muller B. (1990)
  19. Muller , B. , C. Jones , and S. C. West . 1990 . T7 endonuclease I resolves Holliday junctions formed in vitro by RecA protein . Nucleic Acids Res. 18 : 5633 - 5636 . (10.1093/nar/18.19.5633) / Nucleic Acids Res. / T7 endonuclease I resolves Holliday junctions formed in vitro by RecA protein by Muller B. (1990)
  20. Otsuji , N. , H. Iyehara , and Y. Hideshima . 1974 . Isolation and characterization of an Escherichia coli ruv mutant which forms nonseptate filaments after low doses of ultraviolet light irradiation . J. Bacteriol. 117 : 337 - 344 . (10.1128/jb.117.2.337-344.1974) / J. Bacteriol. / Isolation and characterization of an Escherichia coli ruv mutant which forms nonseptate filaments after low doses of ultraviolet light irradiation by Otsuji N. (1974)
  21. Parsons , C. , and S. C. West . 1990 . Specificity of binding to four-way junctions in DNA by bacteriophage T7 endonuclease 1 . Nucleic Acids Res. 18 : 4377 - 4384 . (10.1093/nar/18.15.4377) / Nucleic Acids Res. / Specificity of binding to four-way junctions in DNA by bacteriophage T7 endonuclease 1 by Parsons C. (1990)
  22. Radding , C. M. 1991 . Helical interactions in homologous pairing and strand exchange driven by RecA protein . J. Biol. Chem. 266 : 5355 - 5358 . (10.1016/S0021-9258(19)67599-X) / J. Biol. Chem. / Helical interactions in homologous pairing and strand exchange driven by RecA protein by Radding C. M. (1991)
  23. Sancar , A. , A. M. Hack , and W. D. Rupp . 1979 . Simple method for identification of plasmid-coded proteins . J. Bacteriol. 137 : 692 - 693 . (10.1128/jb.137.1.692-693.1979) / J. Bacteriol. / Simple method for identification of plasmid-coded proteins by Sancar A. (1979)
  24. Sanger , F. , S. Nicklen , and A. R. Coulson . 1977 . DNA sequencing with chain-terminating inhibitors . Proc. Natl. Acad. Sci. USA 74 : 5463 - 5467 . (10.1073/pnas.74.12.5463) / Proc. Natl. Acad. Sci. USA / DNA sequencing with chain-terminating inhibitors by Sanger F. (1977)
  25. Sharples , G. J. , F. E. Benson , G. T. Illing , and R. G. Lloyd . 1990 . Molecular and functional analysis of the ruv region of Escherichia coli K-12 reveals three genes involved in DNA repair and recombination . Mol. Gen. Genet. 221 : 219 - 226 . (10.1007/BF00261724) / Mol. Gen. Genet. / Molecular and functional analysis of the ruv region of Escherichia coli K-12 reveals three genes involved in DNA repair and recombination by Sharples G. J. (1990)
  26. Sharples , G. J. , and R. G. Lloyd . 1990 . A novel repeated DNA sequence located in the intergenic regions of bacterial chromosomes . Nucleic Acids Res. 18 : 6503 - 6508 . (10.1093/nar/18.22.6503) / Nucleic Acids Res. / A novel repeated DNA sequence located in the intergenic regions of bacterial chromosomes by Sharples G. J. (1990)
  27. Shinagawa H. Personal communication.
  28. Shinagawa , H. , K. Makino , M. Amemura , S. Kimura , H. Iwasaki , and A. Nakata . 1988 . Structure and regulation of the Escherichia coli ruv operon involved in DNA repair and recombination . J. Bacteriol. 170 : 4322 - 4329 . (10.1128/jb.170.9.4322-4329.1988) / J. Bacteriol. / Structure and regulation of the Escherichia coli ruv operon involved in DNA repair and recombination by Shinagawa H. (1988)
  29. Shinagawa , H. , T. Shiba , H. Iwasaki , K. Makino , T. Takahagi , and A. Nakata . 1991 . Properties of the Escherichia coli RuvA and RuvB proteins involved in DNA repair, recombination and mutagenesis . Biochimie 73 : 505 - 508 . (10.1016/0300-9084(91)90120-P) / Biochimie / Properties of the Escherichia coli RuvA and RuvB proteins involved in DNA repair, recombination and mutagenesis by Shinagawa H. (1991)
  30. Shurvinton , C. E. , and R. G. Lloyd . 1982 . Damage to DNA induces expression of the ruv gene of Escherichia coli . Mol. Gen. Genet. 185 : 352 - 355 . (10.1007/BF00330811) / Mol. Gen. Genet. / Damage to DNA induces expression of the ruv gene of Escherichia coli by Shurvinton C. E. (1982)
  31. Shurvinton , C. E. , R. G. Lloyd , F. E. Benson , and P. V. Attfield . 1984 . Genetic analysis and molecular cloning of the Escherichia coli ruv gene . Mol. Gen. Genet. 194 : 322 - 329 . (10.1007/BF00383535) / Mol. Gen. Genet. / Genetic analysis and molecular cloning of the Escherichia coli ruv gene by Shurvinton C. E. (1984)
  32. Walker G. C. 1984. Mutagenesis and inducible responses to NOTES 7715
  33. Yanisch-Perron , C. , J. Vieira , and J. Messing . 1985 . Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl 8 and pUC19 vectors . Gene 33 : 103 - 119 . (10.1016/0378-1119(85)90120-9) / Gene / Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl 8 and pUC19 vectors by Yanisch-Perron C. (1985)
Dates
Type When
Created 8 years, 9 months ago (Nov. 4, 2016, 1:58 p.m.)
Deposited 4 years ago (July 29, 2021, 1:54 p.m.)
Indexed 1 year, 3 months ago (May 16, 2024, 2:53 p.m.)
Issued 33 years, 8 months ago (Dec. 1, 1991)
Published 33 years, 8 months ago (Dec. 1, 1991)
Published Print 33 years, 8 months ago (Dec. 1, 1991)
Funders 0

None

@article{Sharples_1991, title={Resolution of Holliday junctions in Escherichia coli: identification of the ruvC gene product as a 19-kilodalton protein}, volume={173}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.173.23.7711-7715.1991}, DOI={10.1128/jb.173.23.7711-7715.1991}, number={23}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Sharples, G J and Lloyd, R G}, year={1991}, month=dec, pages={7711–7715} }