DOI: 10.1128/jb.173.2.926-928.1991. Lipopolysaccharide structure required for in vitro trimerization of Escherichia coli OmpF porin

Lipopolysaccharide structure required for in vitro trimerization of Escherichia coli OmpF porin

10.1128/jb.173.2.926-928.1991

Crossref journal-article

American Society for Microbiology

Journal of Bacteriology (235)

Abstract

Deep rought mutants, which produce very defective lipopolysaccharides, are unable to export normal levels of porins into the outer membrane. In this study, we showed that lipopolysaccharides from such mutants were also unable to facilitate the trimerization, in vitro, of monomeric OmpF porin secreted by spheroplasts of Escherichia coli B/r. In contrast, lipopolysaccharides containing most or all of the core oligosaccharides were able to facilitate trimerization.

Bibliography

Sen, K., & Nikaido, H. (1991). Lipopolysaccharide structure required for in vitro trimerization of Escherichia coli OmpF porin. Journal of Bacteriology, 173(2), 926â928.

Authors 2

K Sen (first)
H Nikaido (additional)

References 22 Referenced 87

Ames , G. F. - L. , E. N. Spudich , and H. Nikaido . 1974 . Protein composition of the outer membrane of Salmonella typhimurium: effect of lipopolysaccharide mutations . J. Bacteriol. 117 : 406 - 416 . (10.1128/jb.117.2.406-416.1974) / J. Bacteriol. / Protein composition of the outer membrane of Salmonella typhimurium: effect of lipopolysaccharide mutations by Ames G. F. (1974)
Datta , D. B. , B. Arden , and U. Henning . 1977 . Major proteins of the Escherichia coli outer envelope membrane as bacteriophage receptors . J. Bacteriol. 131 : 821 - 829 . (10.1128/jb.131.3.821-829.1977) / J. Bacteriol. / Major proteins of the Escherichia coli outer envelope membrane as bacteriophage receptors by Datta D. B. (1977)
Funahara , Y. , and H. Nikaido . 1980 . Asymmetric localization of lipopolysaccharides on the outer membrane of Salmonella typhimurium . J. Bacteriol. 141 : 1463 - 1465 . (10.1128/jb.141.3.1463-1465.1980) / J. Bacteriol. / Asymmetric localization of lipopolysaccharides on the outer membrane of Salmonella typhimurium by Funahara Y. (1980)
Galanos , C. , 0. Luderitz , E. T. Rietschel , and 0. Westphal . 1977 . Newer aspects of the chemistry and biology of bacterial lipopolysaccharides with special reference to their lipid A component . Int. Rev. Biochem. 14 : 239 - 335 . / Int. Rev. Biochem. / Newer aspects of the chemistry and biology of bacterial lipopolysaccharides with special reference to their lipid A component by Galanos C. (1977)
Galanos , C. , 0. Luderitz , and 0. Westphal . 1969 . A new method for the extraction of R lipopolysaccharides . Eur. J. Biochem. 9 : 245 - 249 . (10.1111/j.1432-1033.1969.tb00601.x) / Eur. J. Biochem. / A new method for the extraction of R lipopolysaccharides by Galanos C. (1969)
Jap , B. K. 1989 . Molecular design of PhoE porins and its functional consequences . J. Mol. Biol. 205 : 407 - 419 . (10.1016/0022-2836(89)90351-3) / J. Mol. Biol. / Molecular design of PhoE porins and its functional consequences by Jap B. K. (1989)
Klebba , P. E. , S. A. Benson , S. Bala , T. Abdullah , J. Reid , S. P. Singh , and H. Nikaido . 1990 . Determination of OmpF porin antigenicity and structure . J. Biol. Chem. 265 : 6800 - 6810 . (10.1016/S0021-9258(19)39220-8) / J. Biol. Chem. / Determination of OmpF porin antigenicity and structure by Klebba P. E. (1990)
Koplow , J. , and H. Goldfine . 1974 . Alterations in the outer membrane of the cell envelope of heptose-dependent mutants of Escherichia coli . J. Bacteriol. 117 : 527 - 543 . (10.1128/jb.117.2.527-543.1974) / J. Bacteriol. / Alterations in the outer membrane of the cell envelope of heptose-dependent mutants of Escherichia coli by Koplow J. (1974)
Lindberg , A. A. , and C. G. Hellerqvist . 1971 . Bacteriophage attachment sites, serological specificity, and chemical composition of the lipopolysaccharides of semirough and rough mutants of Salmonella typhimurium . J. Bacteriol. 105 : 57 - 64 . (10.1128/jb.105.1.57-64.1971) / J. Bacteriol. / Bacteriophage attachment sites, serological specificity, and chemical composition of the lipopolysaccharides of semirough and rough mutants of Salmonella typhimurium by Lindberg A. A. (1971)
Metcalfe , M. , and I. B. Holland . 1980 . Synthesis of a major outer membrane porin by E. coli spheroplasts . FEMS Microbiol. Lett. 7 : 111 - 114 . (10.1111/j.1574-6941.1980.tb01587.x) / FEMS Microbiol. Lett. / Synthesis of a major outer membrane porin by E. coli spheroplasts by Metcalfe M. (1980)
Muhlradt , P. F. 1971 . Biosynthesis of Salmonella lipopolysaccharide. Studies on the transfer of glucose, galactose, and phosphate to the core in a cell-free system . Eur. J. Biochem. 18 : 20 - 27 . (10.1111/j.1432-1033.1971.tb01209.x) / Eur. J. Biochem. / Biosynthesis of Salmonella lipopolysaccharide. Studies on the transfer of glucose, galactose, and phosphate to the core in a cell-free system by Muhlradt P. F. (1971)
Muhlradt , P. F. , and J. R. Golecki . 1975 . Asymmetrical distribution and artifactual reorientation of lipopolysaccharides in the outer membrane bilayer of Salmonella typhimurium . Eur. J. Biochem. 51 : 343 - 352 . (10.1111/j.1432-1033.1975.tb03934.x) / Eur. J. Biochem. / Asymmetrical distribution and artifactual reorientation of lipopolysaccharides in the outer membrane bilayer of Salmonella typhimurium by Muhlradt P. F. (1975)
Nikaido , H. , and M. Vaara . 1985 . Molecular basis of bacterial outer membrane permeability . Microbiol. Rev. 49 : 1 - 32 . (10.1128/mr.49.1.1-32.1985) / Microbiol. Rev. / Molecular basis of bacterial outer membrane permeability by Nikaido H. (1985)
Osborn , M. J. 1963 . Studies on the gram-negative cell wall. I. Evidence for the role of 2-keto-3-deoxyoctonate in the lipopolysaccharide of Salmonella typhimurium . Proc. Natl. Acad. Sci. USA 50 : 499 - 506 . (10.1073/pnas.50.3.499) / Proc. Natl. Acad. Sci. USA / Studies on the gram-negative cell wall. I. Evidence for the role of 2-keto-3-deoxyoctonate in the lipopolysaccharide of Salmonella typhimurium by Osborn M. J. (1963)
Reid , J. , H. Fung , K. Gehring , P. E. Klebba , and H. Nikaido . 1988 . Targeting of porin to the outer membrane of Escherichia coli. Rate of trimer assembly and identification of a dimer intermediate . J. Biol. Chem. 263 : 7753 - 7759 . (10.1016/S0021-9258(18)68563-1) / J. Biol. Chem. / Targeting of porin to the outer membrane of Escherichia coli. Rate of trimer assembly and identification of a dimer intermediate by Reid J. (1988)
Schindler , H. , and J. P. Rosenbusch . 1981 . Matrix protein in planar membranes: clusters of channels in a native environment and their functional reassembly . Proc. Natl. Acad. Sci. USA 78 : 2302 - 2306 . (10.1073/pnas.78.4.2302) / Proc. Natl. Acad. Sci. USA / Matrix protein in planar membranes: clusters of channels in a native environment and their functional reassembly by Schindler H. (1981)
Schlecht , S. , and G. Schmidt . 1969 . Moglichkeiten zur Differenzierung von Salmonella-R-Formen mittels Antibiotica und antibakterieller Farbstoffe. Zentralbl. Bakteriol. Parasitenkd . Infektionskr. Hyg. Abt. 1 Orig. 212 : 505 - 511 . / Infektionskr. Hyg. Abt. 1 Orig. / Moglichkeiten zur Differenzierung von Salmonella-R-Formen mittels Antibiotica und antibakterieller Farbstoffe. Zentralbl. Bakteriol. Parasitenkd by Schlecht S. (1969)
Sen , K. , and H. Nikaido . 1990 . In vitro trimerization of OmpF porin secreted by spheroplasts of Escherichia coli . Proc. Natl. Acad. Sci. USA 87 : 743 - 747 . (10.1073/pnas.87.2.743) / Proc. Natl. Acad. Sci. USA / In vitro trimerization of OmpF porin secreted by spheroplasts of Escherichia coli by Sen K. (1990)
Smit , J. , Y. Kamio , and H. Nikaido . 1975 . Outer membrane of Salmonella typhimurium: chemical analysis and freeze-fracture studies with lipopolysaccharide mutants . J. Bacteriol. 124 : 942 - 958 . (10.1128/jb.124.2.942-958.1975) / J. Bacteriol. / Outer membrane of Salmonella typhimurium: chemical analysis and freeze-fracture studies with lipopolysaccharide mutants by Smit J. (1975)
Stocker B. A. D. Personal communication.
Wilkinson , R. G. , P. Gemski , Jr. , and B. A. D. Stocker . 1972 . Non-smooth mutants of Salmonella typhimurium: differentiation by phage sensitivity and genetic mapping . J. Gen. Microbiol. 70 : 527 - 554 . (10.1099/00221287-70-3-527) / J. Gen. Microbiol. / Non-smooth mutants of Salmonella typhimurium: differentiation by phage sensitivity and genetic mapping by Wilkinson R. G. (1972)
Yu , F. , H. Yamada , and S. Mizushima . 1981 . Role of lipopolysaccharide in the receptor function for bacteriophage TuIb in Escherichia coli . J. Bacteriol. 148 : 712 - 715 . (10.1128/jb.148.2.712-715.1981) / J. Bacteriol. / Role of lipopolysaccharide in the receptor function for bacteriophage TuIb in Escherichia coli by Yu F. (1981)

Dates

Type	When
Created	8 years, 9 months ago (Nov. 4, 2016, 4:47 p.m.)
Deposited	4 years, 1 month ago (July 29, 2021, 1:13 p.m.)
Indexed	2 months, 3 weeks ago (June 10, 2025, 2:45 p.m.)
Issued	34 years, 8 months ago (Jan. 1, 1991)
Published	34 years, 8 months ago (Jan. 1, 1991)
Published Print	34 years, 8 months ago (Jan. 1, 1991)

Funders 0

None

BibTeX

@article{Sen_1991, title={Lipopolysaccharide structure required for in vitro trimerization of Escherichia coli OmpF porin}, volume={173}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.173.2.926-928.1991}, DOI={10.1128/jb.173.2.926-928.1991}, number={2}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Sen, K and Nikaido, H}, year={1991}, month=jan, pages={926–928} }

JSON

{
  "indexed": {
    "date-parts": [
      [
        2025,
        6,
        10
      ]
    ],
    "date-time": "2025-06-10T18:45:30Z",
    "timestamp": 1749581130717
  },
  "reference-count": 22,
  "publisher": "American Society for Microbiology",
  "issue": "2",
  "license": [
    {
      "start": {
        "date-parts": [
          [
            1991,
            1,
            1
          ]
        ],
        "date-time": "1991-01-01T00:00:00Z",
        "timestamp": 662688000000
      },
      "content-version": "tdm",
      "delay-in-days": 0,
      "URL": "https://journals.asm.org/non-commercial-tdm-license"
    }
  ],
  "content-domain": {
    "domain": [
      "journals.asm.org"
    ],
    "crossmark-restriction": true
  },
  "published-print": {
    "date-parts": [
      [
        1991,
        1
      ]
    ]
  },
  "abstract": "<jats:p>Deep rought mutants, which produce very defective lipopolysaccharides, are unable to export normal levels of porins into the outer membrane. In this study, we showed that lipopolysaccharides from such mutants were also unable to facilitate the trimerization, in vitro, of monomeric OmpF porin secreted by spheroplasts of Escherichia coli B/r. In contrast, lipopolysaccharides containing most or all of the core oligosaccharides were able to facilitate trimerization.</jats:p>",
  "DOI": "10.1128/jb.173.2.926-928.1991",
  "type": "journal-article",
  "created": {
    "date-parts": [
      [
        2016,
        11,
        4
      ]
    ],
    "date-time": "2016-11-04T20:47:59Z",
    "timestamp": 1478292479000
  },
  "page": "926-928",
  "update-policy": "http://dx.doi.org/10.1128/asmj-crossmark-policy-page",
  "source": "Crossref",
  "is-referenced-by-count": 87,
  "title": "Lipopolysaccharide structure required for in vitro trimerization of Escherichia coli OmpF porin",
  "prefix": "10.1128",
  "volume": "173",
  "author": [
    {
      "given": "K",
      "family": "Sen",
      "sequence": "first",
      "affiliation": [
        {
          "name": "Department of Molecular and Cell Biology, University of California, Berkeley 94720."
        }
      ]
    },
    {
      "given": "H",
      "family": "Nikaido",
      "sequence": "additional",
      "affiliation": [
        {
          "name": "Department of Molecular and Cell Biology, University of California, Berkeley 94720."
        }
      ]
    }
  ],
  "member": "235",
  "reference": [
    {
      "key": "p_1",
      "doi-asserted-by": "crossref",
      "first-page": "406",
      "DOI": "10.1128/jb.117.2.406-416.1974",
      "article-title": "Protein composition of the outer membrane of Salmonella typhimurium: effect of lipopolysaccharide mutations",
      "volume": "117",
      "author": "Ames G. F.",
      "year": "1974",
      "unstructured": "Ames , G. F. - L. , E. N. Spudich , and H. Nikaido . 1974 . Protein composition of the outer membrane of Salmonella typhimurium: effect of lipopolysaccharide mutations . J. Bacteriol. 117 : 406 - 416 .",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "p_2",
      "doi-asserted-by": "crossref",
      "first-page": "821",
      "DOI": "10.1128/jb.131.3.821-829.1977",
      "article-title": "Major proteins of the Escherichia coli outer envelope membrane as bacteriophage receptors",
      "volume": "131",
      "author": "Datta D. B.",
      "year": "1977",
      "unstructured": "Datta , D. B. , B. Arden , and U. Henning . 1977 . Major proteins of the Escherichia coli outer envelope membrane as bacteriophage receptors . J. Bacteriol. 131 : 821 - 829 .",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "p_3",
      "doi-asserted-by": "crossref",
      "first-page": "1463",
      "DOI": "10.1128/jb.141.3.1463-1465.1980",
      "article-title": "Asymmetric localization of lipopolysaccharides on the outer membrane of Salmonella typhimurium",
      "volume": "141",
      "author": "Funahara Y.",
      "year": "1980",
      "unstructured": "Funahara , Y. , and H. Nikaido . 1980 . Asymmetric localization of lipopolysaccharides on the outer membrane of Salmonella typhimurium . J. Bacteriol. 141 : 1463 - 1465 .",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "p_4",
      "first-page": "239",
      "article-title": "Newer aspects of the chemistry and biology of bacterial lipopolysaccharides with special reference to their lipid A component",
      "volume": "14",
      "author": "Galanos C.",
      "year": "1977",
      "unstructured": "Galanos , C. , 0. Luderitz , E. T. Rietschel , and 0. Westphal . 1977 . Newer aspects of the chemistry and biology of bacterial lipopolysaccharides with special reference to their lipid A component . Int. Rev. Biochem. 14 : 239 - 335 .",
      "journal-title": "Int. Rev. Biochem."
    },
    {
      "key": "p_5",
      "doi-asserted-by": "crossref",
      "first-page": "245",
      "DOI": "10.1111/j.1432-1033.1969.tb00601.x",
      "article-title": "A new method for the extraction of R lipopolysaccharides",
      "volume": "9",
      "author": "Galanos C.",
      "year": "1969",
      "unstructured": "Galanos , C. , 0. Luderitz , and 0. Westphal . 1969 . A new method for the extraction of R lipopolysaccharides . Eur. J. Biochem. 9 : 245 - 249 .",
      "journal-title": "Eur. J. Biochem."
    },
    {
      "key": "p_6",
      "doi-asserted-by": "crossref",
      "first-page": "407",
      "DOI": "10.1016/0022-2836(89)90351-3",
      "article-title": "Molecular design of PhoE porins and its functional consequences",
      "volume": "205",
      "author": "Jap B. K.",
      "year": "1989",
      "unstructured": "Jap , B. K. 1989 . Molecular design of PhoE porins and its functional consequences . J. Mol. Biol. 205 : 407 - 419 .",
      "journal-title": "J. Mol. Biol."
    },
    {
      "key": "p_7",
      "doi-asserted-by": "crossref",
      "first-page": "6800",
      "DOI": "10.1016/S0021-9258(19)39220-8",
      "article-title": "Determination of OmpF porin antigenicity and structure",
      "volume": "265",
      "author": "Klebba P. E.",
      "year": "1990",
      "unstructured": "Klebba , P. E. , S. A. Benson , S. Bala , T. Abdullah , J. Reid , S. P. Singh , and H. Nikaido . 1990 . Determination of OmpF porin antigenicity and structure . J. Biol. Chem. 265 : 6800 - 6810 .",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "p_8",
      "doi-asserted-by": "crossref",
      "first-page": "527",
      "DOI": "10.1128/jb.117.2.527-543.1974",
      "article-title": "Alterations in the outer membrane of the cell envelope of heptose-dependent mutants of Escherichia coli",
      "volume": "117",
      "author": "Koplow J.",
      "year": "1974",
      "unstructured": "Koplow , J. , and H. Goldfine . 1974 . Alterations in the outer membrane of the cell envelope of heptose-dependent mutants of Escherichia coli . J. Bacteriol. 117 : 527 - 543 .",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "p_9",
      "doi-asserted-by": "crossref",
      "first-page": "57",
      "DOI": "10.1128/jb.105.1.57-64.1971",
      "article-title": "Bacteriophage attachment sites, serological specificity, and chemical composition of the lipopolysaccharides of semirough and rough mutants of Salmonella typhimurium",
      "volume": "105",
      "author": "Lindberg A. A.",
      "year": "1971",
      "unstructured": "Lindberg , A. A. , and C. G. Hellerqvist . 1971 . Bacteriophage attachment sites, serological specificity, and chemical composition of the lipopolysaccharides of semirough and rough mutants of Salmonella typhimurium . J. Bacteriol. 105 : 57 - 64 .",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "p_10",
      "doi-asserted-by": "crossref",
      "first-page": "111",
      "DOI": "10.1111/j.1574-6941.1980.tb01587.x",
      "article-title": "Synthesis of a major outer membrane porin by E. coli spheroplasts",
      "volume": "7",
      "author": "Metcalfe M.",
      "year": "1980",
      "unstructured": "Metcalfe , M. , and I. B. Holland . 1980 . Synthesis of a major outer membrane porin by E. coli spheroplasts . FEMS Microbiol. Lett. 7 : 111 - 114 .",
      "journal-title": "FEMS Microbiol. Lett."
    },
    {
      "key": "p_11",
      "doi-asserted-by": "crossref",
      "first-page": "20",
      "DOI": "10.1111/j.1432-1033.1971.tb01209.x",
      "article-title": "Biosynthesis of Salmonella lipopolysaccharide. Studies on the transfer of glucose, galactose, and phosphate to the core in a cell-free system",
      "volume": "18",
      "author": "Muhlradt P. F.",
      "year": "1971",
      "unstructured": "Muhlradt , P. F. 1971 . Biosynthesis of Salmonella lipopolysaccharide. Studies on the transfer of glucose, galactose, and phosphate to the core in a cell-free system . Eur. J. Biochem. 18 : 20 - 27 .",
      "journal-title": "Eur. J. Biochem."
    },
    {
      "key": "p_12",
      "doi-asserted-by": "crossref",
      "first-page": "343",
      "DOI": "10.1111/j.1432-1033.1975.tb03934.x",
      "article-title": "Asymmetrical distribution and artifactual reorientation of lipopolysaccharides in the outer membrane bilayer of Salmonella typhimurium",
      "volume": "51",
      "author": "Muhlradt P. F.",
      "year": "1975",
      "unstructured": "Muhlradt , P. F. , and J. R. Golecki . 1975 . Asymmetrical distribution and artifactual reorientation of lipopolysaccharides in the outer membrane bilayer of Salmonella typhimurium . Eur. J. Biochem. 51 : 343 - 352 .",
      "journal-title": "Eur. J. Biochem."
    },
    {
      "key": "p_13",
      "doi-asserted-by": "crossref",
      "first-page": "1",
      "DOI": "10.1128/mr.49.1.1-32.1985",
      "article-title": "Molecular basis of bacterial outer membrane permeability",
      "volume": "49",
      "author": "Nikaido H.",
      "year": "1985",
      "unstructured": "Nikaido , H. , and M. Vaara . 1985 . Molecular basis of bacterial outer membrane permeability . Microbiol. Rev. 49 : 1 - 32 .",
      "journal-title": "Microbiol. Rev."
    },
    {
      "key": "p_14",
      "doi-asserted-by": "crossref",
      "first-page": "499",
      "DOI": "10.1073/pnas.50.3.499",
      "article-title": "Studies on the gram-negative cell wall. I. Evidence for the role of 2-keto-3-deoxyoctonate in the lipopolysaccharide of Salmonella typhimurium",
      "volume": "50",
      "author": "Osborn M. J.",
      "year": "1963",
      "unstructured": "Osborn , M. J. 1963 . Studies on the gram-negative cell wall. I. Evidence for the role of 2-keto-3-deoxyoctonate in the lipopolysaccharide of Salmonella typhimurium . Proc. Natl. Acad. Sci. USA 50 : 499 - 506 .",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "p_15",
      "doi-asserted-by": "crossref",
      "first-page": "7753",
      "DOI": "10.1016/S0021-9258(18)68563-1",
      "article-title": "Targeting of porin to the outer membrane of Escherichia coli. Rate of trimer assembly and identification of a dimer intermediate",
      "volume": "263",
      "author": "Reid J.",
      "year": "1988",
      "unstructured": "Reid , J. , H. Fung , K. Gehring , P. E. Klebba , and H. Nikaido . 1988 . Targeting of porin to the outer membrane of Escherichia coli. Rate of trimer assembly and identification of a dimer intermediate . J. Biol. Chem. 263 : 7753 - 7759 .",
      "journal-title": "J. Biol. Chem."
    },
    {
      "key": "p_16",
      "doi-asserted-by": "crossref",
      "first-page": "2302",
      "DOI": "10.1073/pnas.78.4.2302",
      "article-title": "Matrix protein in planar membranes: clusters of channels in a native environment and their functional reassembly",
      "volume": "78",
      "author": "Schindler H.",
      "year": "1981",
      "unstructured": "Schindler , H. , and J. P. Rosenbusch . 1981 . Matrix protein in planar membranes: clusters of channels in a native environment and their functional reassembly . Proc. Natl. Acad. Sci. USA 78 : 2302 - 2306 .",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "p_17",
      "first-page": "505",
      "article-title": "Moglichkeiten zur Differenzierung von Salmonella-R-Formen mittels Antibiotica und antibakterieller Farbstoffe. Zentralbl. Bakteriol. Parasitenkd",
      "volume": "212",
      "author": "Schlecht S.",
      "year": "1969",
      "unstructured": "Schlecht , S. , and G. Schmidt . 1969 . Moglichkeiten zur Differenzierung von Salmonella-R-Formen mittels Antibiotica und antibakterieller Farbstoffe. Zentralbl. Bakteriol. Parasitenkd . Infektionskr. Hyg. Abt. 1 Orig. 212 : 505 - 511 .",
      "journal-title": "Infektionskr. Hyg. Abt. 1 Orig."
    },
    {
      "key": "p_18",
      "doi-asserted-by": "crossref",
      "first-page": "743",
      "DOI": "10.1073/pnas.87.2.743",
      "article-title": "In vitro trimerization of OmpF porin secreted by spheroplasts of Escherichia coli",
      "volume": "87",
      "author": "Sen K.",
      "year": "1990",
      "unstructured": "Sen , K. , and H. Nikaido . 1990 . In vitro trimerization of OmpF porin secreted by spheroplasts of Escherichia coli . Proc. Natl. Acad. Sci. USA 87 : 743 - 747 .",
      "journal-title": "Proc. Natl. Acad. Sci. USA"
    },
    {
      "key": "p_19",
      "doi-asserted-by": "crossref",
      "first-page": "942",
      "DOI": "10.1128/jb.124.2.942-958.1975",
      "article-title": "Outer membrane of Salmonella typhimurium: chemical analysis and freeze-fracture studies with lipopolysaccharide mutants",
      "volume": "124",
      "author": "Smit J.",
      "year": "1975",
      "unstructured": "Smit , J. , Y. Kamio , and H. Nikaido . 1975 . Outer membrane of Salmonella typhimurium: chemical analysis and freeze-fracture studies with lipopolysaccharide mutants . J. Bacteriol. 124 : 942 - 958 .",
      "journal-title": "J. Bacteriol."
    },
    {
      "key": "p_20",
      "unstructured": "Stocker B. A. D. Personal communication."
    },
    {
      "key": "p_21",
      "doi-asserted-by": "crossref",
      "first-page": "527",
      "DOI": "10.1099/00221287-70-3-527",
      "article-title": "Non-smooth mutants of Salmonella typhimurium: differentiation by phage sensitivity and genetic mapping",
      "volume": "70",
      "author": "Wilkinson R. G.",
      "year": "1972",
      "unstructured": "Wilkinson , R. G. , P. Gemski , Jr. , and B. A. D. Stocker . 1972 . Non-smooth mutants of Salmonella typhimurium: differentiation by phage sensitivity and genetic mapping . J. Gen. Microbiol. 70 : 527 - 554 .",
      "journal-title": "J. Gen. Microbiol."
    },
    {
      "key": "p_22",
      "doi-asserted-by": "crossref",
      "first-page": "712",
      "DOI": "10.1128/jb.148.2.712-715.1981",
      "article-title": "Role of lipopolysaccharide in the receptor function for bacteriophage TuIb in Escherichia coli",
      "volume": "148",
      "author": "Yu F.",
      "year": "1981",
      "unstructured": "Yu , F. , H. Yamada , and S. Mizushima . 1981 . Role of lipopolysaccharide in the receptor function for bacteriophage TuIb in Escherichia coli . J. Bacteriol. 148 : 712 - 715 .",
      "journal-title": "J. Bacteriol."
    }
  ],
  "container-title": "Journal of Bacteriology",
  "original-title": [],
  "language": "en",
  "link": [
    {
      "URL": "https://journals.asm.org/doi/pdf/10.1128/jb.173.2.926-928.1991",
      "content-type": "application/pdf",
      "content-version": "vor",
      "intended-application": "text-mining"
    },
    {
      "URL": "https://journals.asm.org/doi/pdf/10.1128/jb.173.2.926-928.1991",
      "content-type": "unspecified",
      "content-version": "vor",
      "intended-application": "similarity-checking"
    }
  ],
  "deposited": {
    "date-parts": [
      [
        2021,
        7,
        29
      ]
    ],
    "date-time": "2021-07-29T17:13:04Z",
    "timestamp": 1627578784000
  },
  "score": 1,
  "resource": {
    "primary": {
      "URL": "https://journals.asm.org/doi/10.1128/jb.173.2.926-928.1991"
    }
  },
  "subtitle": [],
  "short-title": [],
  "issued": {
    "date-parts": [
      [
        1991,
        1
      ]
    ]
  },
  "references-count": 22,
  "journal-issue": {
    "issue": "2",
    "published-print": {
      "date-parts": [
        [
          1991,
          1
        ]
      ]
    }
  },
  "alternative-id": [
    "10.1128/jb.173.2.926-928.1991"
  ],
  "URL": "http://dx.doi.org/10.1128/jb.173.2.926-928.1991",
  "relation": {},
  "ISSN": [
    "0021-9193",
    "1098-5530"
  ],
  "subject": [],
  "container-title-short": "J Bacteriol",
  "published": {
    "date-parts": [
      [
        1991,
        1
      ]
    ]
  },
  "assertion": [
    {
      "value": "2021-02-02",
      "order": 2,
      "name": "published",
      "label": "Published",
      "group": {
        "name": "publication_history",
        "label": "Publication History"
      }
    }
  ]
}