The Bacillus subtilis sin gene, a regulator of alternate developmental processes, codes for a DNA-binding protein
10.1128/jb.173.2.678-686.1991
Crossref journal-article
American Society for Microbiology
Journal of Bacteriology (235)
Abstract

The sin gene of Bacillus subtilis encodes a dual-function regulatory protein, Sin, which is a negative as well as a positive regulator of alternate developmental processes that are induced at the end of vegetative growth in response to nutrient depletion. Sin has been purified to homogeneity by using a simple two-step procedure. It was found to bind to the developmentally regulated aprE (alkaline protease) gene at two sites in vitro. The stronger Sin-binding site (SBS-1) is located more than 200 bp upstream from the transcription start site. It is required for Sin repression of aprE expression in vivo, as strains bearing SBS-1 deletions were not affected by the sin gene. The second, weaker Sin-binding site lies on a DNA fragment that contains the aprE promoter. Results of DNase I, exonuclease III, and dimethyl sulfate footprinting analysis of SBS-1 suggested that Sin binding involves two adjacent binding sites which appear to contain two different partial dyad symmetries. An analysis of the predicted amino acid sequence of Sin revealed a potential leucine zipper protein dimerization motif which is flanked by two helix-turn-helix motifs that could be involved in recognizing two different dyad symmetries.

Bibliography

Gaur, N. K., Oppenheim, J., & Smith, I. (1991). The Bacillus subtilis sin gene, a regulator of alternate developmental processes, codes for a DNA-binding protein. Journal of Bacteriology, 173(2), 678–686.

Authors 3
  1. N K Gaur (first)
  2. J Oppenheim (additional)
  3. I Smith (additional)
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Dates
Type When
Created 8 years, 9 months ago (Nov. 4, 2016, 4:47 p.m.)
Deposited 4 years ago (July 29, 2021, 1:12 p.m.)
Indexed 2 months, 3 weeks ago (June 3, 2025, 12:28 a.m.)
Issued 34 years, 7 months ago (Jan. 1, 1991)
Published 34 years, 7 months ago (Jan. 1, 1991)
Published Print 34 years, 7 months ago (Jan. 1, 1991)
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@article{Gaur_1991, title={The Bacillus subtilis sin gene, a regulator of alternate developmental processes, codes for a DNA-binding protein}, volume={173}, ISSN={1098-5530}, url={http://dx.doi.org/10.1128/jb.173.2.678-686.1991}, DOI={10.1128/jb.173.2.678-686.1991}, number={2}, journal={Journal of Bacteriology}, publisher={American Society for Microbiology}, author={Gaur, N K and Oppenheim, J and Smith, I}, year={1991}, month=jan, pages={678–686} }